Fully understand drug candidate glycoprofiles with pipeline-specific solutions.
Gather critical high-resolution information and site-specific data points with advanced glycan analysis for complex biologic development.
Glycan analysis
Overview
Glycan analysis provides important information on critical quality attributes (CQAs) that could affect stability, safety and efficacy of a protein therapeutic.
Specific needs in understanding the glycosylation profiles change throughout the drug development process, but the requirement for high-resolution glycan information remains the same and is essential to help ensure product quality.
Address pipeline-specific challenges with glycan analysis workflows based on capillary electrophoresis (CE) and mass spectrometry (MS) to support rapid screening, in-depth characterization and lot-to-lot comparisons.
Manage development timelines with rapid N-linked glycan analysis
Workflow
Find a simpler path to market with scalable N-linked glycan analysis solutions that rapidly identify therapeutic antibody glycosylation profiles.
Thousands of samples, including those containing N-linked glycans, must be screened monthly in the early stages of development, driving the need for high-throughput assays to shorten development time.
Meet the need for large-scale sample analysis and glycan profiling throughout development with robust, high-throughput, CE-based workflows that integrate sample preparation, multi-channel separation and screening assays with intuitive data analysis software for rapid reporting.
- Streamlined sample preparation
- Rapid method development with parallel processing
- High-throughput, parallel screening of released N-linked glycans
- Reproducible migration times and accurate glycan peak assignments
- Ready for integration into automation
Resources
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Technical note
Reproducible glycan analysis for clone selection
Enable separation of a 96-well plate in under 2 hours with an N-linked glycan analysis workflow.
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Technical note
Eliminate labor-intensive bench work and provide a higher throughput in N-linked glycan analysis with highly consistent results
Streamline N-linked glycan analysis using automated sample preparation with a magnetic bead-mediated glycan workflow followed by capillary electrophoresis (CE).
Manage development timelines with rapid N-linked glycan analysis
Suited for:
- High-throughput glycan screening
- Rapid clone selection
BioPhase 8800 system
Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.
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BioPhase Fast Glycan Labeling and Analysis kit
Release and label glycoprotein N-linked glycans for high-resolution capillary electrophoresis using laser-induced fluorescence (LIF) detection and an HR-NCHO separation matrix.
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Confirm glycoform identity with detailed glycosylation analysis
Workflow
Tackle the complex analytical challenges of modern therapeutics.
Intact protein analysis, routinely performed at different stages of monoclonal antibody (mAb) development, is essential for confirming the identity of glycoforms and monitoring product quality. However, increasingly complex glycoform distributions make it challenging to obtain detailed information about glycosylation on complex therapeutic proteins.
LC-MS workflows and data analysis software optimized for biopharma, enable rapid profiling of intact proteins for in-depth characterization. Confident peak assignment and better understanding of the location of glycosylation provide the comprehensive data needed for making critical decisions
- Accurate localization of glycosylation
- Confident differentiation of position isomers
Resources
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Technical note
Accurate peak assignment becomes less daunting with detailed information about glycosylation on complex therapeutic proteins
Enable accurate and comprehensive glycopeptide characterization for routine, in-depth analysis of next-generation protein therapeutics and standard monoclonal antibodies (mAbs).
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Technical note
Facilitate in-depth characterization of therapeutic proteins
Differentiate positional isomers of O-glycopeptides and leverage electron activated dissociation (EAD) to elucidate complex glycosylation profiles in protein therapeutics.
Confirm glycoform identity with detailed glycosylation analysis
Suited for:
- In-depth characterization of glycosylation profiles
- Post-translational modification (PTM) identification
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
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ZenoTOF 7600 system
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
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Biologics Explorer software
Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.
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Monitor product quality during manufacturing
Workflow
Keep pace, with batch comparison workflows designed for confidence in quality.
Routine characterization of biosimilars or batch comparison of manufactured biologic samples is critical for maintaining biotherapeutic quality throughout development and production.
Monitor glycosylation profiles the intact or subunit level with flexible LC-MS solutions providing in-depth characterization and reliable monitoring of known PTMs.
- Simple and accurate monitoring and identification of intact glycoforms
- Capture of all common detectable peptides, including low-level glycopeptides
- Ability to analyze and process large data sets
Monitor product quality during manufacturing
Suited for:
- Routine glycosylation assays
- High-throughput glycoform screening
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
Learn more
ZenoTOF 7600 system
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
Learn more
Biologics Explorer software
Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.
Learn more
All resources
Filter:
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Webinar
Facilitate clone selection with high-throughput glycan profiling
Get insight into high-throughput strategies for glycan analysis to condense development timelines.
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PDF
EAD fragmentation explained
See electron activated dissociation (EAD) fragmentation in action for large molecules, and understand the benefit of EAD for identifying and localizing glycosylation.
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Technical note
Confident identification and characterization of advanced glycation end products
Learn how electron activated dissociation (EAD) enables near-complete fragmentation of advanced glycation end products (AGEs) while retaining the AGE moieties for confident sequence identification, accurate localization
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Technical note
Reproducible glycan analysis for clone selection
Enable separation of a 96-well plate in under 2 hours with an N-linked glycan analysis workflow.
-
Technical note
Eliminate labor-intensive bench work and provide a higher throughput in N-linked glycan analysis
Streamline N-linked glycan analysis using automated sample preparation with a magnetic bead-mediated glycan workflow followed by capillary electrophoresis (CE).
-
Technical note
Accurate peak assignment becomes less daunting with detailed information about glycosylation
Enable accurate and comprehensive glycopeptide characterization for routine, in-depth analysis of next-generation protein therapeutics and standard mAbs.
-
Technical note
Facilitate in-depth characterization of therapeutic proteins
Differentiate positional isomers of O-glycopeptides and leverage electron activated dissociation (EAD) to elucidate complex glycosylation profiles in protein therapeutics.
-
Technical note
Advanced tools for identifying and monitoring CQAs
Reliable processing, visualization, reviewing and reporting tools offer an easy path for decision making in biopharmaceutical development.
Associated applications
Subunit mass analysis and middle-down
Take control of the unknowns. Gain confident sequence confirmation and localization of post-translational modifications (PTMs) at the sub-unit level.
Learn more
Intact protein analysis
Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.
Learn more
Charge heterogeneity analysis
Pursue the end goal with confidence. Achieve the full picture and accelerate decisions with comprehensive characterization of biopharmaceutical charge variants.
Learn more