Increase your released N-glycan screening throughput
Proper N-linked oligosaccharide association with therapeutic proteins is critical for the structure, function and clearance of biologics such as monoclonal antibodies (mAbs). Changes in carbohydrate composition or concentration can significantly impact the overall efficacy of therapeutic proteins and can also lead to unwanted therapeutic side effects. Therefore, high-resolution glycan information is critical for the safety and efficacy of a biotherapeutic.
The BioPhase Fast Glycan Labeling and Analysis kit enables comprehensive, high-resolution characterization of glycosylated biotherapeutics on a diverse array of N-glycan species while maintaining screening throughput.
Glycan characterization in minutes
The BioPhase Fast Glycan Labeling and Analysis kit provides reagents to release and label N-glycans for analysis on the BioPhase 8800 system with ultraviolet (UV) and laser-induced fluorescence (LIF) detection. Sample preparation is performed in an automatable format with PNGase F enzymatic release of N-linked glycans followed by derivation with the charged fluorophore, 9-aminopyrene-1,4,6-trisulfonic acid (APTS).
Designed for the analysis of 200 samples, this kit provides the flexibility and compatibility to achieve high-resolution released N-glycan separations for glycan characterization of biotherapeutics.
BioPhase Fast Glycan Labeling and Analysis kit
Release and label glycoprotein N-linked glycans for high-resolution capillary electrophoresis using laser-induced fluorescence (LIF) detection and an HR-NCHO separation matrix. Simplify glycan identification by automatically comparing normalized electrophoretic mobility against predetermined GU values with BioPhase software.
BioPhase 8800 system
Enable uncompromised accuracy for large sample sets and faster time to answers with parallel processing of 8 samples while retaining the capability to deliver sensitive fast glycan, RNA, capillary electrophoresis sodium dodecyl sulfate (CE-SDS) and capillary isoelectric focusing (cIEF) analysis.
BioPhase BFS cartridge
Achieve purity, charge variant and glycan analysis throughput goals with the pre-built, 8-capillary BioPhase BFS capillary cartridge.
| Learning Center | |
|---|---|
| BioPhase Fast Glycan Labeling and Analysis kit flyer | Download |
| Technical note: Reproducibility study of a high-throughput released N-glycan workflow | Download |
| Technical note: Released N-linked glycan analysis employing automated sample preparation on a Biomek i5 liquid handler | Download |
| Technical note: High-throughput released N-glycan identification using CE and a customizable glycan library | Download |