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High-resolution purity and integrity analysis for long oligonucleotides

Oligo 200 nt Purity and Integrity kit

Single-nucleotide resolution for oligonucleotides, ready to go when you are.

The Oligo 200 nt Purity and Integrity kit is a validated, ready-to-use capillary electrophoresis (CE) solution for high‑resolution oligonucleotide analysis from 10 to 200 nucleotides. Designed for analytical development and quality control of mRNA therapeutics and gene editing programs, this CE kit enables single-nucleotide resolution for single-guide RNA (sgRNA), guide RNA (gRNA), and poly(A) tail length distribution analysis on the BioPhase 8800 system and PA 800 Plus system.

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High-resolution oligonucleotide purity and integrity analysis

Not a new platform. Not a reset. Just sharper insight, applied where it counts.

Enable high-resolution analysis of single-stranded oligonucleotides up to 200 nt, with confident characterization of sgRNA, gRNA, and poly(A) tail length distributions. Designed for use on the BioPhase 8800 system and PA 800 Plus system, this ready-to-use CE kit for oligonucleotides delivers single-nucleotide resolution across an extended size range without complex method development. Gain the reliable and reproducible data that analytical development teams need to move mRNA therapeutic or gene editing programs from process optimization toward late-stage development and QC.

Key features:

  • Single nucleotide resolution across an extended size range

Achieve single base resolution from ~10 to 140 nucleotides, with reliable detection of single stranded oligonucleotides up to 200 nucleotides, supporting detailed purity and integrity assessment of sgRNA, gRNA, and poly(A) tail distributions. 

  • Ready-to-use capillary electrophoresis workflow with minimal method development

Proprietary separation gel is supplied ready to use, no reconstitution or dilution required, reducing assay variability and accelerating deployment in analytical development labs.

  • Compatible with BioPhase 8800 system and PA 800 Plus system

Designed for seamless use across SCIEX CE platforms, enabling method continuity as teams scale from development through later-stage programs or transfer methods between groups. 

  • High sensitivity and robust quantitation

Demonstrates linear response across a wide concentration range with low limits of quantitation, supporting confident detection of low-level impurities and consistent data generation. 

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Compatible products

BioPhase 8800 system

Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.

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PA 800 Plus system

Characterize therapeutic molecules with confidence with the kit-based system.

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BioPhase BFS capillary cartridge - 8 x 50 cm

Pre-assembled bare-fused silica 8-capillary cartridge for the BioPhase 8800 system - 8 x 50 cm. (P/N 5080123)

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BFS capillary cartridge for the PA 800 Plus system

Pre-assembled bare-fused silica capillary cartridge – 30 cm for the PA 800 Plus system.

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Related applications

Messenger RNA analysis

Break through barriers and extend the frontiers of messenger RNA (mRNA), self-amplifying RNA (saRNA) and circular RNA (circRNA) development with intuitive analytical solutions. Confidently innovate with high-quality, sensitive and accurate data to assess integrity, purity and critical quality attributes (CQAs).

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CRISPR/Cas9 analysis

Set your own schedule for CRISPR/Cas9 gene editing with innovative, intuitive analytical solutions. Unleash the potential of your Cas9 messenger RNA (mRNA), single guide RNA (sgRNA) and Cas9 proteins by understanding quality, purity and safety and assessing on/off target effects.

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Resources

Frequently asked questions

The kit supports high-resolution analysis of single-stranded oligonucleotides from approximately 10 to 200 nucleotides, enabling confident purity and integrity assessment across an extended size range.

Yes. The kit is designed for characterization of single-stranded nucleotides, whether they are based on DNA, RNA or a mixture of nucleotides.

Yes. The kit is designed for detailed purity and integrity characterization of sgRNA and gRNA, delivering single-nucleotide resolution ideal for CRISPR and gene editing workflows.

Yes. The Oligo 200 nt Purity and Integrity kit enables accurate poly(A) tail length distribution analysis, supporting analytical development of mRNA therapeutics.

The kit is compatible with the BioPhase 8800 system and the PA 800 Plus system, enabling method continuity across SCIEX CE platforms.

No. The separation gel is supplied ready to use with no reconstitution or dilution required, reducing method development time and improving assay reproducibility to easily establish platform methods.

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