Break through barriers and extend the frontiers of messenger RNA (mRNA), self-amplifying RNA (saRNA) and circular RNA (circRNA) development with intuitive analytical solutions. Confidently innovate with high-quality, sensitive and accurate data to assess integrity, purity and critical quality attributes (CQAs).
Messenger RNA analysis
Explore the latest and trending topics
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Webinar
Beyond mRNA: how advances in analytical techniques are revolutionizing the RNA drug landscape
Self-amplifying RNA (saRNA) can enable lower dosing and expand the range of potential indications. However, srRNAs are often much larger in size (>9,000 bases). Learn how to assess these large molecules with high-resolution assays.
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Technical note
Separation of circRNA from its linear precursor
Pursue improved RNA-based drugs consisting of a continous loop without 3’ poly-A tail or 5’ capping through confident quality assessment. High-quality separation of circRNA from linear precursors let you judge product quality and move projects forward.
Overview
The analysis of mRNA is complex due to its fragile nature, heterogeneity and length.
Discover workflows that let you break through the analytical boundaries of in vitro transcribed (IVT) products and provide quality results to confidently address the challenges of mRNA, circRNA and saRNA development.
Determine CQAs, integrity, purity and secondary structures with intuitive solutions that provide meaningful answers to keep your process in progress.
Perspectives:
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“Given the “one break no effect” with mRNA, the integrity of mRNA drugs must be assessed to ensure its quality. Capillary gel electrophoresis (CGE) is a key tool in this field. CGE systems, such as the BioPhase 8800 system from SCIEX, offering high resolution and efficiently characterizing RNA profiles, enable accurate quality control. Ensuring the integrity and purity of mRNA formulations is essential for the development of effective and safe therapeutics.”
Jérémie Parot, PhD Research Scientist, SINTEF
mRNA integrity and purity
Workflow
mRNA product quality is directly linked to integrity and purity.
Enable control of your RNA products. Assess the purity of full-sized mRNA molecules with the highest quality and resolution, and create pathways for analyzing and understanding these fragile molecules more easily and reliably.
Kit-based solutions let you save time so you can focus on your research and help determine the future of medicine.
- Achieve high-resolution mRNA and impurity separation over an extended size range
- Precise kit solutions with pre-assembled cartridges deliver superior accuracy and precision
- Find relevant impurities (even below 1%) with laser-induced fluorescence (LIF) detection
- Cover your compliance needs through compatibility with the Empower Chromatography Data System (CDS)
mRNA integrity and purity
Suited for:
- High-quality separation
- Rapid method development and sample analysis
BioPhase 8800 system
Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.
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RNA 9000 Purity & Integrity kit
Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.
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BioPhase BFS capillary cartridge
Pre-assembled bare-fused silica 8-capillary cartridge for the BioPhase 8800 system.
Learn more
Featured resources
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Technical note
Assessment of integrity and purity of mRNAs from LNPs
Take control of impurities and artifacts in your mRNA-lipid nanoparticle (LNP) products. Achieve high-quality, robust assessment of the integrity and size of mRNA molecules encapsulated in LNPs.
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Webinar
Assessment of distinct mRNA-LNP formulations by CGE-LIF and implications for RNA-based drugs
Learn from scientist Jérémie Parot (SINTEF) how to break through the boundaries of mRNA analysis.
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Technical note
Compliant-ready, streamlined data management for mRNA analyses with Empower CDS
Set your own schedule for mRNA analyses by streamlining your CE data management with the Empower CDS.
mRNA integrity and purity
Suited for:
- High-quality separation
- Low to medium throughput needs
PA 800 Plus system
Characterize therapeutic molecules with confidence with the kit-based system.
Learn more
RNA 9000 Purity & Integrity kit
Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.
Learn more
BFS capillary cartridge for the PA 800 Plus system
Pre-assembled bare-fused silica capillary cartridge for the PA 800 Plus system.
Learn more
CircRNA
Workflow
The use of next-generation RNA, such as circular RNA (circRNA)—a type of RNA that is not dependent on CQAs such as 3' poly-A tails or 5' capping—is growing.
Pursue improved RNA-based drugs with the ability to confidently separate circRNA from linear precursors and assess a continuous loop of RNA using high-quality data.
- Take control of your circRNA purity with a %CV of <2%
- Rely on superior separation of impurities with highly sensitive detection
- Save time with automated size determination and quantitation of linear RNA precursors
- Cover your compliance needs through compatibility with the Empower CDS
CircRNA
Suited for:
- High-quality separation
- Rapid method development and sample analysis
BioPhase 8800 system
Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.
Learn more
RNA 9000 Purity & Integrity kit
Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.
Learn more
BioPhase BFS capillary cartridge
Pre-assembled bare-fused silica 8-capillary cartridge for the BioPhase 8800 system.
Learn more
PA 800 Plus system
Characterize therapeutic molecules with confidence with the kit-based system.
Learn more
RNA 9000 Purity & Integrity kit
Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.
Learn more
BFS capillary cartridge for the PA 800 Plus system
Pre-assembled bare-fused silica capillary cartridge for the PA 800 Plus system.
Learn more
5’ capping
Workflow
Imagine taking control of your mRNA stability and translation efficiency by better understanding your 5’ CQAs.
Characterize and quantify capped vs. uncapped mRNA species and dig deeper into distinguishing different capping intermediates with high-quality data generated using solutions based on capillary electrophoresis (CE) and accurate mass spectrometry.
- Clearly identify capping intermediates (G cap, cap 0 and cap 1) with accurate mass information
- Break free from quantitation limitations with accurate mass quantitation
- Differentiate capped from uncapped 5’ ends with high-quality CE solutions
- Be prepared for a method transfer to quality control (QC) with reliable CE solutions
5’ capping
Suited for:
- Identification and quantitation of 5'-end capping intermediates
- Robust, analytical flow setup
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
Learn more
X500B QTOF system
A purpose-built QTOF system, designed specifically to accelerate everyday biologics characterization.
Learn more
Molecule Profiler software
Determine impurities and biotransformations for a wide variety of modalities.
Learn more
5’ capping
Suited for:
- Identification and highly sensitive quantitation of capping intermediates
- Meeting flexibility needs to perform a range of additional workflows
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
Learn more
ZenoTOF 7600 system
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
Learn more
Molecule Profiler software
Determine impurities and biotransformations for a wide variety of modalities.
Learn more
5’ capping
Suited for:
- Differentiation of capped from non-capped 5' ends
- Assay transfer to quality control (QC)
PA 800 Plus system
Characterize therapeutic molecules with confidence with the kit-based system.
Learn more
ssDNA 100-R kit
Kit with coated capillaries, gel, and standards to analyze single-stranded nucleic acids on the PA 800 Plus system.
Learn more
3’ poly-A tail
Workflow
Understanding product stability lays the foundation for improved drugs.
Lead the way by characterizing your 3’-end CQAs, whether you are using template-encoded tails, enzymatic addition or a combination of these approaches.
Give your team the power to determine the lengths of the adenosine tails and their distribution profiles in mature mRNA products with solutions based on CE and accurate mass systems.
- Progress your mRNA development with a clear understanding of your poly-A tails
- Access poly-A tail length information via deconvolution of high-quality accurate mass data
- Obtain size information for 3’ tails with excellent base resolution via CE
3’ poly-A tail
Suited for:
- Molecular weight determination of 3'-end poly-A tails
- Robust, analytical flow setup
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
Learn more
X500B QTOF system
A purpose-built QTOF system, designed specifically to accelerate everyday biologics characterization.
Learn more
SCIEX OS software
Unleash the analytical power of the next-generation software platform for data acquisition and processing.
Learn more
3’ poly-A tail
Suited for:
- Molecular weight determination of 3'-end poly-A tails
- Meeting flexibility needs to perform a range of additional workflows
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
Learn more
ZenoTOF 7600 system
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
Learn more
SCIEX OS software
Unleash the analytical power of the next-generation software platform for data acquisition and processing.
Learn more
PA 800 Plus system
Characterize therapeutic molecules with confidence with the kit-based system.
Learn more
ssDNA 100-R kit
Kit with coated capillaries, gel, and standards to analyze single-stranded nucleic acids on the PA 800 Plus system.
Learn more
Secondary structure
Workflow
RNA structures can affect your LNP formulations and the stability and efficacy of a final product. Gain greater control of structural prediction for your oligonucleotides of interest. Leverage solutions that gather empirical data without nucleotide or structural bias to enable better insights into RNA backbone flexibility based on base-paired and unconstrained residues
- Achieve structural understanding with data-driven information
- Find hotspots for backbone flexibility in your nucleic acid products
- Benefit from data without nucleotide bias
Secondary structure
GenomeLab GeXP system
A DNA sequencer and qPCR system in one, with the flexibility to run multiple applications completely unattended.
Learn more
All resources
Filter:
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Solution guide
Development of better LNP-based genetic medicines
Break through the barriers to developing better LNP-based drugs and make better use of your time with streamlined CE and MS workflows for mRNA-LNPs.
-
Webinar
Beyond mRNA: how advances in analytical techniques are revolutionizing the RNA drug landscape
Self-amplifying RNA (saRNA) can enable lower dosing and expand the range of potential indications. However, srRNAs are often much larger in size (>9,000 bases). Learn how to assess these large molecules with high-resolution assays.
-
Technical note
Assessment of integrity and purity of mRNAs from LNPs
Take control of impurities and artifacts in your mRNA-lipid nanoparticle (LNP) products. Achieve high-quality, robust assessment of the integrity and size of mRNA molecules encapsulated in LNPs.
-
Webinar
Assessment of distinct mRNA-LNP formulations by CGE-LIF and implications for RNA-based drugs
Learn from scientist Jérémie Parot (SINTEF) how to break through the boundaries of mRNA analysis.
-
Technical note
Compliant-ready, streamlined data management for mRNA analyses with Empower CDS
Set your own schedule for mRNA analyses by streamlining your CE data management with the Empower CDS.
-
Technical note
Comprehensive method development for a wide size range of single-stranded nucleic acids
Create pathways for effective method development and learn how to achieve the highest quality data for RNA products with sizes ranging from 50–9,000 nucleotides and beyond.
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Technical note
Separation of circular RNA from its linear precursor
Pave the way to better characterization of circRNA generated from linear precursors and take your RNA analysis to the next level.
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Technical note
Understanding secondary structures of single-stranded RNA with chemical probing
Breeze into achieving stable encapsulation of RNA molecules in lipid nanoparticles (LNPs). Be empowered to make informed decisions with structural data on your modalities.
Associated applications
CRISPR/Cas9 analysis
Set your own schedule for CRISPR/Cas9 gene editing with innovative, intuitive analytical solutions. Unleash the potential of your Cas9 messenger RNA (mRNA), single guide RNA (sgRNA) and Cas9 proteins by understanding quality, purity and safety and assessing on/off target effects.
Learn more
Lipid nanoparticles and non-viral carriers
Expand the possibilities of drug delivery with innovative analytical solutions for lipid raw materials, LNPs and other non-viral carriers. Move beyond boundaries in structural elucidation and monitoring of analytes to estabilish confidence in the quality and stablity of your products.
Learn more
Plasmid and DNA analysis
Advance your projects with purpose-built solutions for the analysis of plasmid DNA (pDNA) and double-stranded DNA (dsDNA) to support the development of your messenger RNA (mRNA), viral vectors, recombinant proteins and other new modalities.
Learn more