Request information

Messenger RNA analysis

Break through barriers and extend the frontiers of messenger RNA (mRNA), self-amplifying RNA (saRNA) and circular RNA (circRNA) development with intuitive analytical solutions. Confidently innovate with high-quality, sensitive and accurate data to assess integrity, purity and critical quality attributes (CQAs).

Explore the latest and trending topics

Webinar

Beyond mRNA: how advances in analytical techniques are revolutionizing the RNA drug landscape

Self-amplifying RNA (saRNA) can enable lower dosing and expand the range of potential indications. However, srRNAs are often much larger in size (>9,000 bases). Learn how to assess these large molecules with high-resolution assays.
Technical note

Separation of circRNA from its linear precursor

Pursue improved RNA-based drugs consisting of a continous loop without 3’ poly-A tail or 5’ capping through confident quality assessment. High-quality separation of circRNA from linear precursors let you judge product quality and move projects forward.

Overview Workflows Resources

Overview

The analysis of mRNA is complex due to its fragile nature, heterogeneity and length.

Discover workflows that let you break through the analytical boundaries of in vitro transcribed (IVT) products and provide quality results to confidently address the challenges of mRNA, circRNA and saRNA development.

Determine CQAs, integrity, purity and secondary structures with intuitive solutions that provide meaningful answers to keep your process in progress.

Perspectives:

“Given the “one break no effect” with mRNA, the integrity of mRNA drugs must be assessed to ensure its quality. Capillary gel electrophoresis (CGE) is a key tool in this field. CGE systems, such as the BioPhase 8800 system from SCIEX, offering high resolution and efficiently characterizing RNA profiles, enable accurate quality control. Ensuring the integrity and purity of mRNA formulations is essential for the development of effective and safe therapeutics.”

Jérémie Parot, PhD Research Scientist, SINTEF

Workflows:

mRNA integrity and purity CircRNA 5’ capping 3’ poly-A tail Secondary structure

mRNA integrity and purity

Workflow

mRNA product quality is directly linked to integrity and purity.

Enable control of your RNA products. Assess the purity of full-sized mRNA molecules with the highest quality and resolution, and create pathways for analyzing and understanding these fragile molecules more easily and reliably.

Kit-based solutions let you save time so you can focus on your research and help determine the future of medicine.

Achieve high-resolution mRNA and impurity separation over an extended size range
Precise kit solutions with pre-assembled cartridges deliver superior accuracy and precision
Find relevant impurities (even below 1%) with laser-induced fluorescence (LIF) detection
Cover your compliance needs through compatibility with the Empower Chromatography Data System (CDS)

mRNA integrity and purity

Suited for:

High-quality separation
Rapid method development and sample analysis

BioPhase 8800 system

Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.

RNA 9000 Purity & Integrity kit

Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.

BioPhase BFS capillary cartridge - 8 x 30 cm

Pre-assembled bare-fused silica 8-capillary cartridge for the BioPhase 8800 system - 8 x 30 cm. (P/N 5080121)

Featured resources

mRNA integrity and purity

Suited for:

High-quality separation
Low to medium throughput needs

PA 800 Plus system

Characterize therapeutic molecules with confidence with the kit-based system.

RNA 9000 Purity & Integrity kit

Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.

BFS capillary cartridge for the PA 800 Plus system

Pre-assembled bare-fused silica capillary cartridge for the PA 800 Plus system.

Featured resources

Technical note

Comprehensive method development for a wide size range of single-stranded nucleic acids

Create pathways for effective method development and learn how to achieve the highest quality data for RNA products with sizes ranging from 50–9,000 nucleotides and beyond.

CircRNA

Workflow

The use of next-generation RNA, such as circular RNA (circRNA)—a type of RNA that is not dependent on CQAs such as 3' poly-A tails or 5' capping—is growing.

Pursue improved RNA-based drugs with the ability to confidently separate circRNA from linear precursors and assess a continuous loop of RNA using high-quality data.

Take control of your circRNA purity with a %CV of <2%
Rely on superior separation of impurities with highly sensitive detection
Save time with automated size determination and quantitation of linear RNA precursors
Cover your compliance needs through compatibility with the Empower CDS

CircRNA

Suited for:

High-quality separation
Rapid method development and sample analysis

BioPhase 8800 system

Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.

RNA 9000 Purity & Integrity kit

Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.

BioPhase BFS capillary cartridge - 8 x 30 cm

Pre-assembled bare-fused silica 8-capillary cartridge for the BioPhase 8800 system - 8 x 30 cm. (P/N 5080121)

Featured resources

Technical note

Separation of circular RNA from its linear precursor

Pave the way to better characterization of circRNA generated from linear precursors and take your RNA analysis to the next level.

CircRNA

Suited for:

High-quality separation
Low to medium throughput needs

PA 800 Plus system

Characterize therapeutic molecules with confidence with the kit-based system.

RNA 9000 Purity & Integrity kit

Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.

BFS capillary cartridge for the PA 800 Plus system

Pre-assembled bare-fused silica capillary cartridge for the PA 800 Plus system.

5’ capping

Workflow

Imagine taking control of your mRNA stability and translation efficiency by better understanding your 5’ CQAs.

Characterize and quantify capped vs. uncapped mRNA species and dig deeper into distinguishing different capping intermediates with high-quality data generated using solutions based on capillary electrophoresis (CE) and accurate mass spectrometry.

Clearly identify capping intermediates (G cap, cap 0 and cap 1) with accurate mass information
Break free from quantitation limitations with accurate mass quantitation
Differentiate capped from uncapped 5’ ends with high-quality CE solutions
Be prepared for a method transfer to quality control (QC) with reliable CE solutions

5’ capping

Suited for:

Identification and quantitation of 5'-end capping intermediates
Robust, analytical flow setup

ExionLC AE system

Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.

X500B QTOF system

A purpose-built QTOF system, designed specifically to accelerate everyday biologics characterization.

Molecule Profiler software

Determine impurities and biotransformations for a wide variety of modalities.

5’ capping

Suited for:

Identification and highly sensitive quantitation of capping intermediates
Meeting flexibility needs to perform a range of additional workflows

ExionLC AE system

Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.

ZenoTOF 7600 system

A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.

Molecule Profiler software

Determine impurities and biotransformations for a wide variety of modalities.

5’ capping

Suited for:

Differentiation of capped from non-capped 5' ends
Assay transfer to quality control (QC)

PA 800 Plus system

Characterize therapeutic molecules with confidence with the kit-based system.

ssDNA 100-R kit

Kit with coated capillaries, gel, and standards to analyze single-stranded nucleic acids on the PA 800 Plus system.

3’ poly-A tail

Workflow

Understanding product stability lays the foundation for improved drugs.

Lead the way by characterizing your 3’-end CQAs, whether you are using template-encoded tails, enzymatic addition or a combination of these approaches.

Give your team the power to determine the lengths of the adenosine tails and their distribution profiles in mature mRNA products with solutions based on CE and accurate mass systems.

Progress your mRNA development with a clear understanding of your poly-A tails
Access poly-A tail length information via deconvolution of high-quality accurate mass data
Obtain size information for 3’ tails with excellent base resolution via CE

3’ poly-A tail

Suited for:

Molecular weight determination of 3'-end poly-A tails
Robust, analytical flow setup

ExionLC AE system

Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.

X500B QTOF system

A purpose-built QTOF system, designed specifically to accelerate everyday biologics characterization.

SCIEX OS software

Unleash the analytical power of the next-generation software platform for data acquisition and processing.

3’ poly-A tail

Suited for:

Molecular weight determination of 3'-end poly-A tails
Meeting flexibility needs to perform a range of additional workflows

ExionLC AE system

Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.

ZenoTOF 7600 system

A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.

SCIEX OS software

Unleash the analytical power of the next-generation software platform for data acquisition and processing.

3’ poly-A tail

Suited for:

Achieving the highest resolution power
QC deployment

PA 800 Plus system

Characterize therapeutic molecules with confidence with the kit-based system.

ssDNA 100-R kit

Kit with coated capillaries, gel, and standards to analyze single-stranded nucleic acids on the PA 800 Plus system.

All resources

Filter:

Associated applications

CRISPR/Cas9 analysis

Set your own schedule for CRISPR/Cas9 gene editing with innovative, intuitive analytical solutions. Unleash the potential of your Cas9 messenger RNA (mRNA), single guide RNA (sgRNA) and Cas9 proteins by understanding quality, purity and safety and assessing on/off target effects.

Lipid nanoparticles and non-viral carriers

Expand the possibilities of drug delivery with innovative analytical solutions for lipid raw materials, LNPs and other non-viral carriers. Move beyond boundaries in structural elucidation and monitoring of analytes to estabilish confidence in the quality and stablity of your products.

Plasmid and DNA analysis

Advance your projects with purpose-built solutions for the analysis of plasmid DNA (pDNA) and double-stranded DNA (dsDNA) to support the development of your messenger RNA (mRNA), viral vectors, recombinant proteins and other new modalities.

SCIEX Now support network

The destination for all your support needs.

Contact support