iCIEF-MS technology

Fast track characterization of multiple attributes of intact proteins

Accelerate your biotherapeutic development by directly coupling iCIEF (imaged capillary isoelectric focusing) charge variant analysis with high-resolution mass spectrometry detection for intact proteins.

The Intabio microfluidic, chip-based, integrated iCIEF-MS system enables the identification of post-translational modifications including glycosylation and charge variants that can impact the safety and efficacy of biotherapeutic drugs.

Intabio is now part of SCIEX. Learn more here.

Patented microfluidic chip-based technology makes it all possible

The IntaBio system’s proprietary microfluidic chip is the driving technology enabling iCIEF-MS analysis of intact biologics. The microfluidic chip in the IntaBio system will separate charge variants by capillary isoelectric focusing, enable UV quantification and perform electrospray ionization (ESI) directly into a mass spectrometer for further mass analysis, all with the use of MS compatible reagents.

Once focusing is complete, an electrolyte is introduced via a side channel to re-ionize the charge variants and the electric field is re-oriented to initiate the mobilization of the peaks toward the electrospray “ESI” tip. This novel mobilization process ensures that peak resolution is maintained until the ESI tip is reached. Near the ESI tip, a second channel introduces a mobilizer to enable ESI and ionization efficiency.