Identity/Charge Heterogeneity – Remarkably Fast, Accurate and Easy to Use

cIEF and CZE Charge Heterogeneity Analysis of Biologics

When working with a monoclonal antibody, you need to know the different charge variants to determine identity and stability. You also need the utmost confidence in your results, as the success of your biologic can depend on the accuracy of your results.

Whether you have several methods to develop, samples to QC with limited resources or labs around the world that all need to use the same methods, you need a solution that you can trust. You need to develop charge heterogeneity methods with high resolution and precision that are fast and easy to use, as well as easily transferable across the globe.

The SCIEX PA 800 Plus allows you to confidently assess charge heterogeneity with ultra-high resolution (CIEF) or simplify your sample preparation (CZE). The PA 800 Plus simplifies your workflows and streamlines your methods so you can improve lab productivity and performance. The system also provides you with the raw data required for regulatory acceptance.

Discover how CE-MS can enable next-generation biotherapeutics

Explore the wide selection of capillary electrophoresis-mass spectrometry (CE-MS) solutions that SCIEX offers to solve your challenges for next-generation biotherapeutics, from early discovery through development.

Get Proven, Fast Accurate Results with CZE

Labs that need to analyze mAbs for identity and stability can rapidly obtain high resolution charge heterogeneity information with simple sample preparation, using CZE. You can also develop methods with high resolution and precision, that are fast and easy to use, as well as easily transferable across the globe.

PA 800 Plus

Flexible platform for multiple analytical assays with a temperature controlled capillary for the utmost reproducibility.

EZ-CE Cartridge

With an inventory of just the EZ-CE cartridge you can run CE-SDS, Fast Glycan, and Rapid Charge Variant analyses.

CZE Rapid Charge Variant Analysis Kit

Charge variant analysis has never been easier. No sample buffer is required; simply dilute the sample in water and you are ready for your separation.

Webinar

Intercompany Study to Evaluate the Robustness of CZE Technology for the Heterogeneity Analysis of mAbs

Webinar

MAb charge heterogeneity analysis - A case Study from Merck Sharp & Dohme

Webinar

MAb charge heterogeneity analysis - A Test Method Fit for QC Testing

Webinar

Simple and fast charge variants analysis of mAbs by CZE with Dr. Yan He from Pfizer

Ultra-high Resolution with cIEF

For maximum sensitivity, ultra-high-resolution capillary isoelectric focusing (cIEF) is capable of achieving separation between isoforms as closely related as 0.03 pI units. The SCIEX cIEF workflow on the PA 800 Plus System has proven both robust and portable. Universal or platform methods can also be created – significantly decreasing method development efforts and simplifying workflows with a single method for molecules across a wide pI range.

PA 800 Plus

Flexible platform for multiple analytical assays with a temperature controlled capillary for the utmost reproducibility.

Advanced cIEF Starter Kit

With an inventory of just the EZ-CE cartridge you can run CE-SDS, Fast Glycan, and Rapid Charge Variant analyses.

pI Marker Kit

pI Marker Kit features synthetic peptides for high resolution and quantitative reproducibility in calculating protein pI.

Technical Note

Identification of System Parameters Critical for High-Performance cIEF

Technical Note

High-Resolution cIEF of Therapeutic Monoclonal Antibodies: A Platform Method Covering pH 4-10

Technical Note

A Robust cIEF Method: Intermediate Precision for the pH 5-7 Range

Technical Note

Achieving Robust cIEF Analysis of Monoclonal Antibodies While Increasing Capillary Run-Life and Maintaining High Resolution