Pursue the end goal with confidence. Achieve the full picture and accelerate decisions with comprehensive characterization of biopharmaceutical charge variants.
Charge heterogeneity analysis
Explore the latest and trending topics
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Technical note
A Rapid, high-resolution lot release assay for co-formulated mAbs with CZE
Discover a novel workflow for high-speed lot release assay using capillary zone electrophoresis on the PA800 Plus system. This kit-based workflow provides quantitative results with simple sample preparation.
-
Technical note
Gain insight into mAb structural changes with a CE subunit analysis workflow
Use CE-SDS and cIEF strategies to understand the structural changes that may occur in different domains of the antibody, and leverage this orthogonal approach to better assign charge variant identification
Overview
Break through barriers to quickly and confidently make decisions to push the best drug candidates forward from the start.
Charge heterogeneity analysis enables an understanding of critical attributes that could impact protein therapeutic stability. Early understanding and continued monitoring of a candidates charge profile is important to reduce risk and compress drug development timelines.
In order to make informed decisions on drug candidate developability early, there is a need for comprehensive charge variant analysis with detection of charge variants, and direct identification of what those variants are in a timeline keeping with the fast-pace of biotherapeutic development.
Forge a new path with a clearer understanding of the attributes that impact protein therapeutic stability from early stages. Confidently detect and fully identify charge variants during cell-line and early product or process development phases with optimized CE and MS based workflows that speed up timelines without compromising quality.
Charge variant identification on a single platform
Workflow
Prepare for any scenario, make informed decisions on product developability early with comprehensive charge variant analysis.
Interpreting the structural differences of charge variant components can take weeks and requires the use of multiple analytical methods and instruments, creating a bottleneck in gaining critical information.
Decrease the chance of product stability or safety issues, enable the study of more conditions during cell line development with a fully integrated icIEF-UV/MS workflow for charge heterogeneity analysis.
The Integrated icIEF-UV/MS workflow provides multiple pieces of critical data upfront without manual sample manipulation. Quickly and confidently accelerate candidate selection by achieving separation, quantitation, and identification of biopharmaceutical charge variants and their proteoforms in minutes.
- Significant reduction in time to result from weeks to minutes
- Confident characterization and identification of proteoforms with MS
- Reproducible quantitation of charge variants
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Technical note
Separate, characterize, identify, across a wide mAb pI range of 7-10
A single platform icIEF-UV/MS workflow offers high sensitivity and selectivity to detect low-abundant proteoforms that could impact product quality.
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Technical note
Bring the precision of MS identification to early development
Highly confident identification of CQAs with an integrated icIEF-UV/MS solution that identifies low abundant PTMs at the intact protein level.
Charge variant identification on a single platform
Suited for:
- Comprehensive characterization of charge variant-associated quality attributes
- Charge variant analysis throughout development
Intabio ZT system
Eliminate the guess work and monitor biologics at the intact-protein level, gaining insights into charge variant-associated quality attributes.
Learn more
Biologics Explorer software
Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.
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High-resolution charge profile monitoring
Workflow
Navigate analytical challenges for a wider variety of new modality drug candidates.
The characterization of charge heterogeneity and determination of isoelectric point (pI) adds a critical dimension to establishing identity, purity, post-translational modification and stability of therapeutic protein preparations. Characterization and monitoring of protein therapeutic charge variants can be challenging with the growing number and wider variety of new modality drug candidates.
Adapt to the ever-changing pipeline with a platform capillarty isoelectric focusing (cIEF) workflow designed to deliver high-resolution, and reproducible data allowing for easier scale up in routine use environments. cIEF workflows leverage a single separation method to perform cIEF analysis of multiple mAbs across a broad pH range. The workflow is greatly simplified by the use of a platform method, resulting in reduction or elimination of method optimization and easier scale up for deployment in routine environments.
- Achieve ultra-high-resolution separation between isoforms
- Be confident in your results with reproducible, accurate pI determination
- Scale cIEF analysis for routine use with a simple platform method
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Technical note
Take back time - with multifactor evaluation in one day
Discover a workflow to streamline evaluation of multiple factors in one set of experiments reducing method optimization from weeks to just hours.
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Technical note
Accelerate development of biologics with confidence
Rapid protein therapeutic charge variant characterization across a wider range of modalities. Expand your capacity to discover and develop more successful drug candidates.
High-resolution charge profile monitoring with cIEF
Suited for:
- High-resolution charge variant monitoring
- Rapid screening assays throughout cell line development
BioPhase 8800 system
Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.
Learn more
BioPhase Capillary Isoelectric Focusing (cIEF) kit
Experimental protein isoelectric focusing point (pI) and analysis of charge variants.
Learn more
Rapid charge profile assessment
Workflow
Take back your time with fast, easy to use CZE solutions that are simple to transfer globally.
Analyze mAb charge variants for identity and stability with high resolution in a shorter amount of time.
- High throughput charge heterogenity information
- Save time with fast, simple sample preparation
- Develop simple, transferrable methods
Fast and simple charge profile assessment with CZE
Suited for:
- Comprehensive characterization of charge variant-associated quality attributes
- Charge variant analysis throughout development
PA 800 Plus system
Characterize therapeutic molecules with confidence with the kit-based system.
Learn more
Featured resources
Filter:
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Technical note
Bring the precision of MS identification to early development
Highly confident identification of CQAs with an integrated icIEF-UV/MS solution that identifies low abundant PTMs at the intact protein level.
-
Technical note
Develop a single protocolfor mAb characterization
Robust cIEF platform solutions that mitigate operator error and system variation. Move candidates along with greater confidence and easier portability.
-
Technical note
Reproducible separation and identification of proteoforms
Gain advantages in data quality and time with an integrated system that offers high-resolution and high-throughput separation of intact mAbs and their charge variants.
-
Technical note
Separate, characterize, identify, across a wide pI range
A single platform icIEF-UV/MS workflow offers high sensitivity and selectivity to detect low-abundant proteoforms that could impact product quality.
-
Technical note
Confidently identify glycation events using icIEF-UV/MS
Comprehensive characterization and identification of the charge variants of deglycosylated NISTmAb in minutes.
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Technical note
Quantify charge variants in their native state
Capillary Zone Electrophoresis (CZE) combines the benefits of a native state alaysis with the speed and resolution expected of a capillary electrophoresis.
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Webinar
Enable mass identification of charge variants and modifications on intact ADCs
Reduce charge heterogeneity analysis from weeks to minutes. Identify and monitor the charged modification on ADC payloads in near real-time.
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Webinar
Charge variant identification in minutes
Probe the cause of color change in protein therapeutics and learn how a cell line development group is utilizing the integrated icIEF-UV/MS workflow to monitor advanced glycation events.
-
Webinar
Simplify charge variant characterization during process development
Discover how you can gain multiple pieces of critical information early in process development to assess the impact of cell culture conditions on mAb quality and production.
All resources
Filter:
-
Technical note
Separate, characterize, identify, across a wide mAb pI range of 7-10
A single platform icIEF-UV/MS workflow offers high sensitivity and selectivity to detect low-abundant proteoforms that could impact product quality.
-
Technical note
Bring the precision of MS identification to early development
Highly confident identification of CQAs with an integrated icIEF-UV/MS solution that identifies low abundant PTMs at the intact protein level.
-
Technical note
Accelerate development of biologics with confidence
Rapid protein therapeutic charge variant characterization across a wider range of modalities. Expand your capacity to discover and develop more successful drug candidates.
-
Technical note
Take back time - with multifactor evaluation in one day
Discover a workflow to streamline evaluation of multiple factors in one set of experiments reducing method optimization from weeks to just hours.
-
Technical note
Develop a single protocolfor mAb characterization
Robust cIEF platform solutions that mitigate operator error and system variation. Move candidates along with greater confidence and easier portability.
-
Technical note
Reproducible separation and identification of proteoforms
Gain advantages in data quality and time with an integrated system that offers high-resolution and high-throughput separation of intact mAbs and their charge variants.
-
Technical note
Separate, characterize, identify, across a wide pI range
A single platform icIEF-UV/MS workflow offers high sensitivity and selectivity to detect low-abundant proteoforms that could impact product quality.
-
Technical note
Confidently identify glycation events using icIEF-UV/MS
Comprehensive characterization and identification of the charge variants of deglycosylated NISTmAb in minutes.
-
Technical note
Quantify charge variants in their native state
Capillary Zone Electrophoresis (CZE) combines the benefits of a native state alaysis with the speed and resolution expected of a capillary electrophoresis.
-
Webinar
Enable mass identification of charge variants and modifications on intact ADCs
Reduce charge heterogeneity analysis from weeks to minutes. Identify and monitor the charged modification on ADC payloads in near real-time.
-
Webinar
Charge variant identification in minutes
Probe the cause of color change in protein therapeutics and learn how a cell line development group is utilizing the integrated icIEF-UV/MS workflow to monitor advanced glycation events.
-
Webinar
Simplify charge variant characterization during process development
Discover how you can gain multiple pieces of critical information early in process development to assess the impact of cell culture conditions on mAb quality and production.
-
Technical note
Confidently assign proteoforms and low abundant charge variants without fraction collection
A single platform icIEF-UV/MS workflow on the Intabio ZT system provides confident identification of sialyated glycoproteins.
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Technical note
Enhance PQA identification during ADC development with confident proteoform ID, and payload localization.
Gain a level of characterization beyond traditional LC separation and CID based workflows to provide critical information to guide analytical strategies throughout ADC development.
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Technical note
Charge heterogeneity monitoring to comprehensive biotherapeutic characterization
Take full advantage of advanced characterization capabilities of the EAD enabled ZenoTOF 7600 system with simple conversion from icIEF-UV/MS to EAD based LC/MS.
-
Technical note
Gain insight into mAb structural changes with a CE subunit analysis workflow
Use CE-SDS and cIEF strategies to understand the structural changes that may occur in different domains of the antibody, and leverage this orthogonal approach to better assign charge variant identification
-
Technical note
Enhanced sensitivity to identify low abundant proteoforms
A 4x increase in sensitivity enables detection and confident identification of challenging proteoforms like deamidation
-
Technical note
A Rapid, high-resolution lot release assay for co-formulated mAbs with CZE
Discover a novel workflow for high-speed lot release assay using capillary zone electrophoresis on the PA800 Plus system. This kit-based workflow provides quantitative results with simple sample preparation.
Associated applications
Intact protein analysis
Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.
Learn more
Multi-attribute methodology
Pursue simpler, faster and more accurate product quality profiles. Reclaim time for innovation and reduce overall analytical testing burden with software solutions for product quality attribute (PQA) monitoring.
Learn more