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Differential mobility spectrometry (DMS) and
ion mobility spectrometry are analytical techniques used to separate
ions based on their gas phase mobility. Multiple types of ion
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Differential Ion Mobility.
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LC-MS/MS assay development can be challenging, particularly for analytes that coelute with contaminants or become buried within background noise. The SCIEX SelexION differential mobility separation device (DMS) provides an extra level of selectivity so you can resolve difficult analytes and achieve higher confidence in detection and quantitation. Designed to install between the ionization source and the vacuum interface of your mass spectrometer, SelexION provides a further degree of separation beyond retention time and mass. SelexION uses gas phase differential ion mobility to enable the separation of coeluting compounds, isobaric and isomeric compounds, and compounds buried within high background noise. Instead of spending more time on LC separation strategies and sample preparation, SelexION simplifies your workflow with an innovative add-on separation solution. The SelexION differential mobility separation device:
Require more information?
SelexION can enhance analyte detection, improve quantitation limits, simplify sample preparation, and shorten assay development time. With applications in food and environmental analysis, forensics, lipidomics, peptide and protein quantitation, and small molecule bioanalysis and metabolism, SelexION DMS can enrich your assays, streamline your workflows, and improve your overall productivity.
Easy to install
Compact and simple design allows the cell to be installed without the use of any tools and in less than 2 minutes
Robust and stable
Updated curtain plate to accommodate the differential ion mobility cell. Maintains the same level of robustness and stability associated with the original design
Combine sensitivity and selectivity with unmatched reproducibility and robustness
For scientists who are challenged by assays with isobaric interferences and difficult-to-separate co-eluting contaminants, this fast, reproducible and easy-to-use approach will enhance the selectivity of your LC-MS/MS separations.
Learn More >
Enhance selectivity without the need for additional complex sample preparation
Perform fast separation analyses compatible with UHPLC timescales
Overcome co-eluting matrix interferences and improve data quality in complex samples
Separate and quantify isobaric molecular species for more accurate biological insights
Maximize mass spec workflow flexibility with easy install that doesn’t require breaking the system vacuum
Used in pharmaceutical, academic and food and environment testing labs across the globe, SelexION DMS technology brings the power of differential ion mobility to even routine users. The technology is compatible with several SCIEX Triple Quad™, QTRAP and TripleTOF platforms. Explore the table below to see what SelexION device is right for your system.
We also offer the SelexION+ ion mobility cell, currently available for use on the SCIEX 6500+ series mass spectrometers. The SelexION+ device is enhanced by a jet injector that reduces the ion residence time within fringing fields at the DMS inlet. This delivers a 2-fold increase in transmission over the standard SelexION device.
Read more about SelexION technology >
From pharmaceutical drug discovery/development and lipidomic analysis to food, environmental testing and so much more, learn how SelexION technology has enhanced results in these key applications.
Improve lipid class separation for more confident lipid ID
Get a complete solution for lipidomics research
Improve selectivity for quantitative bioanalysis
Identify and quantify food contaminants
SelexION differential ion mobility technology takes some of the world's most advanced mass spectrometry systems and adds a new dimension of selectivity to enhance quantitative and qualitative performance. Compatible with many SCIEX systems, SelexION device can be installed and uninstalled within 2-minutes without breaking vacuum, giving you the freedom to use ion mobility technology as needed.
Most sensitive and selective system to push quantitation further than ever before
Sensitivity and performance to easily meet analytical and regulatory demands for low-level trace detection.
Achieve the optimum of quantitative performance
Highest sensitivity high-resolution mass system for comprehensive data acquisition
High-resolution mass system with ultra-fast acquisition speeds
SCIEX SelexION device is a small, planar mobility cell that significantly increases analytical separation power, even if compounds have identical molecular weights and chromatographic retention times, made possible by a mobility region and optional polar modifiers in the transport region.Watch the Video >
Two parallel flat plates define a mobility region, allowing nitrogen carrier gas to create forward directional flow between the ion source and the mass spec analyzer. Analytes are separated based on their respective size, shape, charge state and chemical interaction, prior to entering the mass spec.
Polar modifiers in the transport region can lead to a significant increase in analyte separation and peak capacity. Chemical modifiers, such as isopropanol or acetonitrile, influence ion mobility based on their ability to cluster with analytes.
See how the small, planar SelexION DMS device can enhance analytical separations for isobaric species and co-eluting contaminants.
SelexION offers a unique approach to separation that is unmatched by any other available method. Firstly, it is a planar device that separates ions based on differences in mobility in two different regions of the field dependent mobility curve (Ref 1). Secondly, due to its size, the system can operate with very short residence times with optimal performance when using chemical modifiers, and can be used in transparent mode to allow for maximal workflow flexibility.Learn More >
Other types of standard low field mobility devices, such as drift tubes, separate ions based upon differences in the low field mobility constant related to analyte mass, and are generally less effective at separating isobaric ions (Refs 2, 3).
Planar devices such as SelexION eliminates heterogeneous electric fields within the device, leading to increased resolving power compared to curved surface mobility devices (Refs 4, 5).
SelexION offers the ability to turn off separation voltage and operate in a ‘transparent’ mode to transmit all ions, providing the option to perform non-ion mobility assays without removing the front-end device (Ref 1).
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