Differential Ion Mobility Technology

A new dimension in selectivity with SelexION® DMS

Differential mobility spectrometry (DMS) and ion mobility spectrometry are analytical techniques used to separate hard to resolve ions based on their gas-phase mobility. Multiple types of ion mobility devices exist, such as drift tubes, traveling wave, and high-field asymmetric waveform devices. However, none carry the power of SCIEX SelexION® Technology that delivers highly-selective, robust and powerful ion separation that significantly enhances your quantitative and qualitative performance.

The SelexION® Differential Mobility Device can help you address your biggest analytical challenges:

  • Overcome co-eluting matrix interferences and improve data quality in complex samples
  • Separate isobaric compounds for increased confidence in detection
  • Detect and quantify isobaric lipid molecular species for more accurate biological insights
  • Reduce background noise that may be limiting your LOQ
  • Save time with simplified sample preparation
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The power of SelexION takes you beyond limitation

Overcome your most advanced analytical challenges

For scientists who are challenged by assays with isobaric interferences and difficult-to-separate co-eluting contaminants, this fast, reproducible and easy-to-use approach will enhance the selectivity of your LC-MS/MS separations.

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Save time

Enhance selectivity without the need for additional complex sample preparation

Fast separation

Perform fast separation analyses compatible with UHPLC timescales

Improve LOQ

Overcome co-eluting matrix interferences and improve data quality in complex samples

Boost confidence

Separate and quantify isobaric molecular species for more accurate biological insights

2-minute install

Maximize mass spec workflow flexibility with easy install that doesn’t require breaking the system vacuum

The science behind unmatched separation

SCIEX SelexION device is a small, planar mobility cell that significantly increases analytical separation power, even if compounds have identical molecular weights and chromatographic retention times, made possible by a mobility region and optional polar modifiers in the transport region.

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Mobility separation

Two parallel flat plates define a mobility region, allowing nitrogen carrier gas to create forward directional flow between the ion source and the mass spec analyzer. Analytes are separated based on their respective size, shape, charge state and chemical interaction, prior to entering the mass spec.

Modifier separation

Polar modifiers in the transport region can lead to a significant increase in analyte separation and peak capacity. Chemical modifiers, such as isopropanol or acetonitrile, influence ion mobility based on their ability to cluster with analytes.

SelexION in action

See how the small, planar SelexION DMS device can enhance analytical separations for isobaric species and co-eluting contaminants.

A step above the rest: The better way to see more

SelexION offers a unique approach to separation that is unmatched by any other available method. Firstly, it is a planar device that separates ions based on differences in mobility in two different regions of the field dependent mobility curve (Ref 1). Secondly, due to its size, the system can operate with very short residence times with optimal performance when using chemical modifiers, and can be used in transparent mode to allow for maximal workflow flexibility.

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Enhanced isobaric separation

Other types of standard low field mobility devices, such as drift tubes, separate ions based upon differences in the low field mobility constant related to analyte mass, and are generally less effective at separating isobaric ions (Refs 2, 3).

Planar resolving power

Planar devices such as SelexION eliminates heterogeneous electric fields within the device, leading to increased resolving power compared to curved surface mobility devices (Refs 4, 5).

Separation flexibility

SelexION offers the ability to turn off separation voltage and operate in a ‘transparent’ mode to transmit all ions, providing the option to perform non-ion mobility assays without removing the front-end device (Ref 1).

SelexION applications and workflows

Bring the power of SelexION Technology to your application

From pharmaceutical drug discovery/development and lipidomic analysis to food, environmental testing and so much more, learn how SelexION technology has enhanced results in these key applications.

Lipidomic analysis

Improve lipid class separation for more confident lipid ID

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Novel lipid screening

Get a complete solution for lipidomics research

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Bioanalytical quantitation

Improve selectivity for quantitative bioanalysis

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Food contaminants

Identify and quantify food contaminants

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Biological matrices

Successful separation of diastereomeric monohydroxy Flubatine standards

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Get more from your mass spec

The best-in-class mass specs just got better

SelexION differential ion mobility technology takes some of the world's most advanced mass spectrometry systems and adds a new dimension of selectivity to enhance quantitative and qualitative performance. Compatible with many SCIEX systems, SelexION device can be installed and uninstalled within 2-minutes without breaking vacuum, giving you the freedom to use ion mobility technology as needed.

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References:

  1. Schneider, B. B., Nazarov, E. G., Londry, F., Vouros, P. and Covey, T. R. (2016), Differential mobility spectrometry/mass spectrometry history, theory, design optimization, simulations, and applications. Mass Spec Rev, 35: 687–737.
  2.  Shvartsburg AA, Prior DC, Tang K, Smith RD, “High-Resolution differential Ion Mobility Separations Using Planar Analyzers at Elevated Dispersion Fields”, Anal. Chem., 2010, 82, 7649-7655.
  3.  Revercomb and Mason, “Theory of plasma chromatography/gaseous electrophoresis”, Anal. Chem., 47, 1975, 970-983.
  4.  Shvartsburg AA, Li F, Tang K, Smith RD, “High-Resolution Field Asymmetric Waveform Ion Mobility Spectrometry Using New Planar Geometry Analyzers”, Anal. Chem., 2006, 78, 3706-3714.
  5.  Krylov EV, Nazarov EG, Miller RA, “Differential Mobility Spectrometer: Model of Operation”, Int. J. Mass Spectrom., 266, 2007, 76-85.
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