AB Sciex is doing business as SCIEX. © 2010-2019 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.
SelexION Technology

SelexION Technology on QTRAP 5500

SelexION Technology

Differential Ion Mobility Technology

Differential Ion Mobility Technology

A New Dimension in Selectivity with SelexION® DMS

Differential mobility spectrometry (DMS) and ion mobility spectrometry are analytical techniques used to separate hard to resolve ions based on their gas phase mobility. Multiple types of ion mobility devices exist, such as drift tubes, traveling wave, and high-field asymmetric waveform devices. However, none carry the power of SCIEX SelexION® technology that delivers highly-selective, robust and powerful ion separation that significantly enhances your quantitative and qualitative performance.

The SelexION® Differential Mobility Device can help you address your biggest analytical challenges:

  • Overcome co-eluting matrix interferences and improve data quality in complex samples
  • Separate isobaric compounds for increased confidence in detection
  • Detect and quantify isobaric lipid molecular species for more accurate biological insights
  • Reduce background noise that may be limiting your LOQ
  • Save time with simplified sample preparation

The Power of SelexION Takes You Beyond Limitation

SelexION Applications and Workflows

Get More from Your Mass Spec

References:
  1. Schneider, B. B., Nazarov, E. G., Londry, F., Vouros, P. and Covey, T. R. (2016), Differential mobility spectrometry/mass spectrometry history, theory, design optimization, simulations, and applications. Mass Spec Rev, 35: 687–737.
  2. Shvartsburg AA, Prior DC, Tang K, Smith RD, “High-Resolution differential Ion Mobility Separations Using Planar Analyzers at Elevated Dispersion Fields”, Anal. Chem., 2010, 82, 7649-7655.
  3. Revercomb and Mason, “Theory of plasma chromatography/gaseous electrophoresis”, Anal. Chem., 47, 1975, 970-983.
  4. Shvartsburg AA, Li F, Tang K, Smith RD, “High-Resolution Field Asymmetric Waveform Ion Mobility Spectrometry Using New Planar Geometry Analyzers”, Anal. Chem., 2006, 78, 3706-3714.
  5. Krylov EV, Nazarov EG, Miller RA, “Differential Mobility Spectrometer: Model of Operation”, Int. J. Mass Spectrom., 266, 2007, 76-85.

RUO-MKT-18-2036-D

Resources
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Title Date
Separation of Ephedrine and Pseudoephedrine Isomers Using SCIEX SelexION Differential Mobility Technology 02/15/2019
Separation of Bile Acid Isomers with Differential Mobility Spectrometry 12/03/2018
Resolution of Ether and Diacyl-Linked Phospholipids by Differential ION Mobility Spectrometry (DMS) 11/19/2018
Sensitive and Accurate Quantitation of Retinoic Acids in Biological Samples with QTRAP 6500 and SelexION Technology 11/12/2018
SelexION Technology: A New Solution to Selectivity Challenges in Quantitative Bioanalysis 11/06/2018
Differential Ion Mobility Separation of Iso-Elemental Lipid Species 11/02/2018
Separation of Diastereomeric Flubatine Metabolites using SelexION® Technology 10/16/2018
Differential Mobility Spectrometry Resolves Chlorine- and Acetate- Adducts of Phosphatidylcholine (PC) 10/09/2018
Differential Ion Mobility Separation of Glycosylceramides (Cerebrosides) SelexION Technology 09/01/2018
SelexION Odyssey 08/15/2018
Exploring the Figures of Merit of Differential Mobility Spectrometry (DMS) Cells of Varied Geometries 06/06/2018
Using Differential Mobility Spectrometry and Machine Learning-Based Modelling to Predict the Physicochemical Properties of Molecules 06/05/2018
Differential Mobility Separation for Improving Lipidomic Analysis by Mass Spectrometry 03/05/2018
Differential Mobility Spectrometry Analysis of Glycans and Glycopeptides 01/31/2018
Differential Mobility Spectrometry Theory 12/20/2017
Selexion dms technology increasing the selectivity of lc msms analysis 12/06/2017
Omics Applications-SelexION® Differential Mobility Spectrometry Technology 10/11/2017
User Guide: SelexION and SelexION+ Technology Troubleshooting 09/30/2017
Bioanalysis Quantitation Applications eBook 09/25/2017
Determination of Limaprost in Human Plasma 09/13/2017
Selexion Valproic Acid Tech Note 08/31/2017
Solutions for Biotherapeutic Characterization 08/01/2017
SCIEX SelexION Series Ion Mobility System Declaration of Conformance 06/30/2017
Differential Mobility Separation with SCIEX SelexION Technology – A Novel Technique for the Bioanalysis of Poor Fragmenting Molecules Like Valproic Acid 06/29/2017
The Use of SelexION® to Increase Sensitivity for Trace Level Analysis of Small Polar Molecules 06/21/2017
Differential Mobility Spectrometry Resolves Isobaric Metabolite Overlap 06/15/2017
SCIEX Quantitation and Metabolism Solutions Brochure 05/26/2017
SelexION Differential Ion Mobility Technology 05/01/2017
SelexION Differential Ion Mobility Intro 04/10/2017
SelexION Differential Mobility Technology Address your Biggest Analytical Challenges 04/01/2017
Advanced Biotransform Solution Guide 03/28/2017
Dysregulated Lipid Profiles of Non-Alcoholic Fatty Liver Disease (NAFLD) 12/05/2016
Differential Mobility Separation Mass Spectrometry for Quantitation of Large Peptides in Biological Matrices 12/01/2016
SelexION ION Mobility Technology - A New Dimension in Selectivity 11/28/2016
LC-MS/MS Quantitation of Underivatized Glyphosate and Other Polar Pesticides 06/01/2016
Novel Chemical Standards Kits Enable Facile Lipid Quantitation 05/01/2016
Quantification of Peptides with Poor MS/MS Fragmentation using Novel Jet Injector SelexION® + MIM Workflow 01/31/2016
Automating Optimization of Compensation Voltages for Differential Mobility Separation using Skyline 12/11/2015
Multiple Mass Spectrometric Strategies for High-Selectivity Quantification of Proteins and Peptides 12/02/2015
Rapid Quantitation of Substance P in Plasma using Differential Mobility Spectrometry and Micro Flow Liquid Chromatography 12/01/2015
Using Differential Mobility Spectrometry to Separate and Localize Sites of Post-Translational Modifications on Peptides 11/01/2015
Quantitative Lipid Analysis using MRM and Differential Ion Mobility Spectrometry (DMS) 09/01/2015
Resolution of Sphingomyelins in Complex Lipid Extracts by Differential Ion Mobility Spectrometry (DMS) 09/01/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems User Guide 08/31/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems User Guide (ES) 08/31/2015
SelexION_brochure 08/01/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems User Guide (IT) 07/31/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems User Guide (DE) 07/31/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems User Guide (FR) 07/31/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems Site Planning Guide 06/30/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems Site Planning Guide (DE) 06/30/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems Site Planning Guide (FR) 06/30/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems Site Planning Guide (IT) 06/30/2015
User Guide: SelexION Technology for 5500 and 6500 Systems and SelexION+ Technology for 6500+ Systems Site Planning Guide (ES) 06/30/2015
User Guide: SelexION Technology for TripleTOF Systems User Guide 05/31/2015
User Guide: SelexION Technology for TripleTOF Systems User Guide (DE) 05/31/2015
User Guide: SelexION Technology for TripleTOF Systems User Guide (IT) 05/31/2015
User Guide: SelexION Technology for TripleTOF Systems User Guide (FR) 05/31/2015
SelexION Technology for TripleTOF Systems User Guide (ES) 05/31/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide (DE) 03/31/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide 03/31/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide (FR) 03/31/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide (IT) 03/31/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide (ES) 03/31/2015
Caring for TurboV Ion Source Electrodes 01/01/2014
Triple Quad™ 5500 LC/MS/MS System and SelexIONTM technology 01/01/2014
SelexION Technology User Guide 05/10/2013
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selexion-intro-icontop-5col-1

Overcome Your Most Advanced Analytical Challenges

For scientists who are challenged by assays with isobaric interferences and difficult-to-separate co-eluting contaminants, this fast, reproducible and easy-to-use approach will enhance the selectivity of your LC-MS/MS separations.

Learn More

Save Time

Save Time

Enhance selectivity without the need for additional complex sample preparation

Fast Separation

Fast Separation

Perform fast separation analyses compatible with UHPLC timescales

Improve LOQ

Improve LOQ

Overcome co-eluting matrix interferences and improve data quality in complex samples

Boost Confidence

Boost Confidence

Separate and quantify isobaric molecular species for more accurate biological insights

2-Minute Install

2-Minute Install

Maximize mass spec workflow flexibility with easy install that doesn’t require breaking the system vacuum

selexion-intro-icontop-3col-1

The Science Behind Unmatched Separation

SCIEX SelexION device is a small, planar mobility cell that significantly increases analytical separation power, even if compounds have identical molecular weights and chromatographic retention times, made possible by a mobility region and optional polar modifiers in the transport region.

Watch the Video >

Mobility Separation

Mobility Separation

Two parallel flat plates define a mobility region, allowing nitrogen carrier gas to create forward directional flow between the ion source and the mass spec analyzer. Analytes are separated based on their respective size, shape, charge state and chemical interaction, prior to entering the mass spec.

Modifier Separation

Modifier Separation

Polar modifiers in the transport region can lead to a significant increase in analyte separation and peak capacity. Chemical modifiers, such as isopropanol or acetonitrile, influence ion mobility based on their ability to cluster with analytes.

SelexION in Action

SelexION in Action

See how the small, planar SelexION DMS device can enhance analytical separations for isobaric species and co-eluting contaminants.

selexion-intro-icontop-3col-2

A Step Above the Rest: The Better Way to See More

SelexION offers a unique approach to separation that is unmatched by any other available method. Firstly, it is a planar device that separates ions based on differences in mobility in two different regions of the field dependent mobility curve (Ref 1). Secondly, due to its size, the system can operate with very short residence times with optimal performance when using chemical modifiers, and can be used in transparent mode to allow for maximal workflow flexibility.

Learn More

Enhanced Isobaric Separation

Enhanced Isobaric Separation

Other types of standard low field mobility devices, such as drift tubes, separate ions based upon differences in the low field mobility constant related to analyte mass, and are generally less effective at separating isobaric ions (Refs 2, 3).

Planar Resolving Power

Planar Resolving Power

Planar devices such as SelexION eliminates heterogeneous electric fields within the device, leading to increased resolving power compared to curved surface mobility devices (Refs 4, 5).

Separation Flexibility

Separation Flexibility

SelexION offers the ability to turn off separation voltage and operate in a ‘transparent’ mode to transmit all ions, providing the option to perform non-ion mobility assays without removing the front-end device (Ref 1).

selexion-intro-iconleft-1col

Explore SelexION Device Compatibility

Used in pharmaceutical, academic and food and environment testing labs across the globe, SelexION DMS technology brings the power of ion mobility to even routine users. The technology is compatible with several SCIEX Triple Quad™, QTRAP® and TripleTOF® platforms. Explore the table below to see what SelexION device is right for your system.

SelexION+ ion mobility cell is also available for use on SCIEX 6500+ Series mass spectrometers. The SelexION+ device is enhanced by a jet injector that reduces the ion residence time within fringing fields at the DMS inlet. This delivers a 2-fold increase in transmission over the standard SelexION device.

Read More About SelexION+ Technology >

selexion-app-table
InstrumentSelexIONSelexION+
5500 Series ✔
6500 Series ✔
6500+ Series ✔
TripleTOF 5600+ ✔
TripleTOF 6600 ✔
selexion-intro-icontop-5col-2

Bring the Power of SelexION Technology to Your Application

From pharmaceutical drug discovery/development and lipidomic analysis to food, environmental testing and so much more, learn how SelexION technology has enhanced results in these key applications.

Lipidomic Analysis

Lipidomic Analysis

Improve lipid class separation for more confident lipid ID

Read Tech Note
Novel Lipid Screening

Novel Lipid Screening

Get a complete solution for lipidomics research

Read Tech Note
Bioanalytical Quantitation

Bioanalytical Quantitation

Improve selectivity for quantitative bioanalysis

Read Tech Note
Food Contaminants

Food Contaminants

Identify and quantify food contaminants

Read Tech Note
Metabolite Identification

Metabolite Identification

Separate isobaric metabolites

Read Tech Note
selexion-intro-icontop-5col-3

The Best-in-Class Mass Specs Just Got Better

SelexION Differential Ion Mobility Technology takes some of the world's most advanced mass spectrometry systems and adds a new dimension of selectivity to enhance quantitative and qualitative performance. Compatible with many SCIEX systems, SelexION device can be installed and uninstalled within 2-minutes without breaking vacuum, giving you the freedom to use ion mobility technology as needed.

QTRAP 6500+

QTRAP 6500+

Most sensitive and selective system to push quantitation further than ever before

Learn More
QTRAP 6500

QTRAP 6500

High performance quantitative system

Learn More
QTRAP 5500

QTRAP 5500

Designed to excel at MetID, pesticides and biomarker verification

Learn More
TripleTOF 6600

TripleTOF 6600

Highest sensitivity high-resolution mass system for comprehensive data acquisition

Learn More
TripleTOF 5600+

TripleTOF 5600+

High-resolution mass system with ultra-fast acquisition speeds

Learn More
selexion-learning-center
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AB Sciex is doing business as SCIEX. © 2010-2019 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.