Engineered to balance the priorities of speed, data quality, and useability, the SCIEX ZenoTOF 7600+ system is a versatile, high resolution QTOF with high sensitivity and outstanding quantitative and qualitative performance.
When critical decisions depend on your data, you need multi-dimensional insights and multi-acquisition confidence.
Welcome to the future of transformative research, where the numbers that count are the seconds from data to insight, the accuracy and precision of molecular insights, and the cost of efficiency.
The ZenoTOF 7600+ system is more than a mass spectrometer, it’s a platform designed to evolve with your science. By combining proven accuracy, multiomics versatility, and streamlined usability, it empowers researchers to deliver biologically relevant insights with confidence, speed, and adaptability.
Continuing the (r)evolution in quantitative accuracy
The SCIEX ZenoTOF 7600+ system builds off the heritage of robust, accurate quantitation and takes reproducible, high-quality data for both qualitative and quantitative workflows to the next level. This foundation ensures confidence in results for regulatory-compliant studies and exploratory research alike.
Adaptability is at the heart of the ZenoTOF 7600+ system. With support for DIA and DDA acquisition strategies, the system enables comprehensive coverage across proteomics, metabolomics, lipidomics, and applied markets like clinical research, food and environmental testing. And now with the selectivity of the scanning quadrupole dimension with ZT Scan DIA 2.0, run targeted and untargeted workflows alike, achieving exceptional compound coverage and quantitative capabilities at the same time. Then, with tunable fragmentation modes like EAD, gain structural insights and isomeric differentiation.
Zeno trap-enabled DIA
A step change in SWATH data independent acquisition (DIA), leverage the advanced technologies of Zeno SWATH DIA and ZT Scan DIA 2.0 for an increase in biological insights, speed of analysis and accuracy of results
Tunable electron fragmentation of all molecule types
Fast, reagent free, and easy to use, electron activated dissociation (EAD) offers complementary fragmentation information to further extend analytical capabilities beyond traditional CID-based techniques
Scanning speeds of up to 858 Hz
High duty cycles at faster acquisition speeds. For larger cohorts this can allow up to ten times faster throughput than conventional DIA approaches without compromising data quality
TwinSpray and Optiflow Turbo V
Dual sources incorporate the reliability and efficiency of the renowned Turbo V source, while providing flexibility for quickly switching flow rates, including nanoflow regimes for the highest sensitivity needs
Extraordinary flexibility - wherever your science leads
The ZenoTOF 7600+ system is built for flexibility, delivering extraordinary performance when you need it most. Switch seamlessly between DIA for comprehensive coverage, EAD for structural clarity, and Zeno trap-enabled sensitivity for low-abundance targets. This flexibility unlocks new discovery-driven approaches while complementing existing workflows for routine quantitation. Accurate mass performance delivered in one flexible platform that adapts to your workflow needs.
Versatility for multiomics and applied workflows
The ZenoTOF 7600+ system combines high sensitivity MS/MS, tunable electron-capture dissociation (EAD) fragmentation capabilities, and innovative developments in data-independent acquisition (DIA) approaches powered by the Zeno trap. |
Technical notes
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Selecting the right method for your quantitative proteomics experiment just got easier with three different ZT Scan DIA methods ready to use
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Leverage the speed and sensitivity with simultaneous CID- and EAD-cased fragmentation to achieve robust, high-throughput analysis of hormonal steroids in human plasma
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See significant LLOQ improvements in pesticide residue testing in matrices like olive oil
Figure 1. Overview of ZT Scan DIA methodology. Here, three pre-set ZT Scan DIA methods are shown based on user-defined estimated peak width at half height (PWHH), which is dependent on the desired chromatographic separation regime. |
Figure 2. XICs of positive ion TOF MS (top) and TOF MS/MS (bottom) spectra using the MRMHR scan mode to detect steroids in NIST 1950 plasma extract. The y-axis for each spectrum is expanded 10-fold. |
Figure 3. In-matrix LLOQ distribution of calculated replicate injections (n=6) of the olive oil matrix-matched curve using quantifier ion, processed according to SANTE/12862/219 guidelines. >90% of analytes meet two ion criteria below 10 ppb.
Adaptability for evolving research
Science doesn’t stand still and neither should your technology. The ZenoTOF 7600+ system scales effortlessly from untargeted discovery to routine quantitation, allowing researchers to pivot between targeted assays and deep biological exploration without changing platforms. |
Technical notes
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EAD-based middle-down workflows lead to high sequence coverage, accurate localization of labile PTMs and rapid mapping of intrachain disulfide bonds
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Achieve high sequence coverage, accurate drug-antibody ratio (DAR) determination, and precise payload and PTM localizations, among other important characterization information with the EAD data that's easy to run
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Detect and quantify bile acids with a sensitivity compared to that of high-end triple quadrupole instruments, while generating diagnostic fragment ions enabling the distinction between bile acid isomers
Figure 1. EAD middle-down approach resulted in extensive fragmentation of the disulfide-linked LC subunit of T-DXd. The detection of z-series fragments confirmed the payload conjugation of the C-terminal Cys residue. The peaks labeled with an * are the precursor or charge-reduced species.
Figure 2. EAD-based reduced and non-reduced peptide mapping workflows enable comprehensive characterization of Cys-linked ADC's such as trastuzumab deruxtecan (Enhertu, T-DXd) |
Figure 3. XIC chromatogram of bile acids in human plasma. Eleven of 15 targeted bile acids were detected in a single rat plasma sample. In the figure, the major peaks are labeled, and the concentrations (µM) of each bile acid ± the standard deviation are shown in the inset table. |
Streamlined integration and workflow efficiency
Powered by SCIEX OS software, the ZenoTOF 7600+ system simplifies data processing, instrument health monitoring, and workflow automation. This integration reduces onboarding time and complexity, enabling rapid implementation and high-confidence results from day one. Plus, a high-degree of flexibility to perform both targeted and untargeted workflows provides users a complete solution within SCIEX OS software. |
Technical notes
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Carry out rapid characterization of PFAS in cosmetic products using non-targeted acquisition with compound identification and diagnostic fragment ion confirmation
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Perform in-depth characterization of novel psychoactive substances with Zeno EAD DDA methods for increased unique diagnostic fragments in fast DDA drug screening workflows in SCIEX OS software
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SCIEX OS software allows targeted identification and quantitation of untargeted screening of metabolites
Figure 1. Instrumental and data processing workflow for the characterization of PFAS in cosmetics. |
Figure 2. EAD enabled the identification of the correct chlorofentanyl isobar in a discarded postmortem case sample. (Above) The acquired CID spectra gave 3 possible identifications, including a chlorofentanyl isobar, whereas the EAD spectra (below) showed 2 unique fragment ions that provided unambiguous evidence for the identification of ortho-chlorofentanyl |
Figure 3. Targeted and untargeted metabolite screening workflows on the ZenoTOF 7600 system |
Take your biology beyond ID numbers
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"Whether confirming if a biomarker is the right target, validating a new translational biomarker as a drug discovery candidate, or deciding a pathway for therapeutic efficacy, the decisions you make count. ZT Scan DIA will help researchers extract insightful biological answers from its multidimensional data. "
Jose Castro-Perez Vice President, Product Management at SCIEX.
Features
Identify and quantify significantly more analytes in a shorter time, with less sample and higher precision.
ZT Scan DIA 2.0
ZT Scan DIA 2.0 employs linking Q1 data to generate data to MS/MS data - and delivers depth of analysis typical of DIA methods, the precision of targeted approaches, and selectivity that DDA is known to deliver, in one run. |
Zeno TRAP
The Zeno Trap is an accumulation and pulsing device set up orthogonally to the QTOF region that increases the duty cycle of the instrument by >90%, delivers better sensitivity 5-20x and up to 20x more fragment ions. |
Scan speeds of up to 858 Hz
When using ZT Scan DIA 2.0, the isolation window for MS/MS slides along the m/z range of interest during each cycle. With a quadrupole scan speed of up to 4000 Da/s, the system can operate at a MS/MS scan rate of up to 858 Hz, dependent on the predefined mass range (not accounting for multiple precursors in a single isolation encoded window).
Ionization sources
Dual sources incorporate the reliability and efficiency of the renowned Turbo V source, while providing flexibility for quickly switching flow rates, including nanoflow regimes for the highest sensitivity needs |
Tunable electron fragmentation of all molecule types
Exclusive to SCIEX, the tunable EAD cell is unique to other electron fragmentation solutions commercially available. It can maintain labile modifications, delivering new insights that CID cannot. It's fast (~10-30ms), reagent free, and easy to use and implement in a method. |
Zeno SWATH DIA
With all the benefits SWATH DIA coupled with activation of the Zeno trap, Zeno SWATH DIA combines the sensitivity of the Zeno trap with the reproducibility and precision of SWATH DIA, increasing the MS/MS sensitivity for each variable window acquired. Uses less sample and gain more compound identifications. |
Deliver on important timelines
With up to 10-fold improvement in throughput for protein quantitation, the ZenoTOF 7600+ enables 1-minute gradient analyses using ZT Scan DIA 2.0 for low to moderate protein loads.
Maximize specificity
Performance gains with fast LC gradients on the ZenoTOF 7600+ system increase as sample loading and complexity increase when using ZT Scan DIA 2.0, with added specificity from the scanning quadrupole dimension.
SCIEX OS software
Engineered to deliver more.
Faster.
The SCIEX ZenoTOF 7600+ system is powered by SCIEX OS: an intuitive software with a graphical user interface that feels instantly familiar. It is intelligently designed to support our users’ experimental goals and is Windows 11 compatible. |
Multi-inlet support offers flexibility and customization and supports a wide range of flow rate regimes
- Method optimization tool for complex methods including DDA / IDA and SWATH DIA. Optimization tool for complex methods inc. DDA / IDA and SWATH DIA
Get quantitative results quickly by pulling and using specific fragment ions to search a given dataset before integration and combining of the fragment ions
Go from raw data to a quantitative result in minutes as opposed to hours. SCIEX OS analytics enables users to quickly obtain quantitative measurements for targets obtained from large, targeted datasets, according to customer's unique conforming data requirements
SCIEX understands the need for third-party processing applications. The data API within SCIEX OS software can allow access many of our customer’s favorite third-party data-processing tools
Events and webinars
Extraordinary Science
Delve into the world of “extraordinary science” and check out our collection of videos from esteemed scientists on a journey of omics exploration and discovery. |
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First impressions of ZT Scan DIA
Listen to Tim Heyman, from the Mann Lab at the Max Planck Institute of Biochemistry speak about his first impressions of seeing performance gains using ZT Scan DIA. |
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Enhancing quantitative proteomics
Check out Ludwig Roman Sinn, from the Ralser and Demichev Labs at Charité – Universitätsmedizin Berlin share his experience using EAD and ZT Scan DIA for quantitative proteomics |
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Advancing pancreatic cancer research
Hear directly from Mohammadreza Dorvash from the Purcell Lab at Monash University about seeing improvements in speed, precision, and accuracy of his immunopeptidomics experiments |
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Resources
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Technical note
Improved proteomics performance at high throughput using ZT Scan DIA on the ZenoTOF 7600+ system
ZT Scan DIA enhances protein and peptide precursor ID by up to 70%, handles up to 500 samples per day and improves specificity in complex samples with fast LC gradients.
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Technical note
Improved proteomics performance for low sample loadings using ZT Scan DIA on the ZenoTOF 7600+ system
ZT Scan DIA simplifies setup with pre-defined methods, enhances protein and peptide ID by up to 50% at sub-ng loadings and delivers results up to 3 times faster than conventional methods.
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Technical note
Selecting optimal ZT Scan DIA acquisition methods on the ZenoTOF 7600+ system for quantitative proteomics
This technical note compares three ZT Scan DIA methods on the ZenoTOF 7600+ to help users select the appropriate method for their quantitative proteomics experiments.
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Technical note
Improved protein sequence coverage and reduced protein identification FDR
With ZT Scan DIA, the ZenoTOF 7600+ system enhances protein sequence coverage and identification confidence. Achieving up to 7-fold better protein sequence coverage and lower FDR, it outperforms conventional DIA methods.
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Infographic
ZT Scan DIA: The smart hybrid
Learn how ZT Scan DIA is different and how you get coverage, speed, and precision together in one method.
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Hub
Electron activated dissociation
Explore more about how EAD is breaking ground for tandem MS/MS applications from everything to small molecules to large molecule workflows.
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