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High-quality DNA analyses are easier than ever before (1)

DNA 20 kb Plasmid and Linear kit

Plasmid DNA (pDNA) can be a critical starting material, an intermediate product or a drug substance itself. The quality of pDNA is crucial for high-efficiency manufacturing of proteins, viral vectors and in vitro transcribed RNA (IVT RNA).

Leave behind the manual assembly of capillary cartridges for your pDNA assays and low-resolution methods that do not provide relevant answers. Join the future with the DNA 20 kb Plasmid and Linear kit, compatible with the BioPhase 8800 system and the PA 800 Plus system.

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Determining the purity of various plasmid constructs does not need to be a headache. Plasmid isoforms of 2,000–19,000 base pairs (bp)—including supercoiled, linear and open circular species—can be separated and quantified with exceptional resolution and reproducibility using pre-built bare-fused silica (BFS) capillary cartridges.

Learn how capillary gel electrophoresis can automate the sizing of linear double-stranded DNA (dsDNA) and the purity determination of linearized DNA from 100 bp to 20,000 bp, helping you stay focused on the science. Plasmid identification—including highly repetitive long sequence —and residual nucleic acid analyses can be performed with the DNA 20 kb Plasmid and Linear kit.

Key workflows

pDNA purity

Determine pDNA purity easily by leveraging high-quality separation of isoforms across a wide size range. 

  • pDNA purity data image

    dsDNA sizing

    Rely on excellent reproducibility of high-resolution dsDNA sizing and linearization efficiency analyses.

    • dsDNA sizing data image

      Plasmid ID

      Level up your resolution with 50 cm pre-assembled bare-fused silica cartridges for ultra-high separation power of restriction maps.

      • Plasmid ID data image

        Residual nucleic acids

        Perform residual nucleic acid analysis without sequence bias and obtain quantitative information and sizing. 

        • Residual nucleic acids data image

          Content at a glance

          The choice is yours. Stay flexible! The DNA 20 kb Plasmid and Linear kit covers the analysis of 200 samples, while the DNA 20 kb Plasmid and Linear gel and conditioning pack covers the analysis of 400 samples and enables you to choose your favorite stain and standard.

          Content

          Kit

          P/N 5311708

          Pack

          P/N 5312283

          DNA 20 kb Plasmid and Linear gel

          		✔✔
          DNA 20 kb Plasmid test mix✔X
          DNA 20 kb Plasmid and Linear conditioning solution✔✔

          DNA 20 kb Plasmid and Linear sample buffer

          		✔✔
          CE Grade water✔X
          0.1N HCl✔X
          SYBR™ Gold Nucleic Acid gel stain2✔X
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          Pre-built BFS cartridges, compatible with the DNA 20 kb Plasmid and Linear kit and the DNA 20 kb Plasmid and Linear gel and conditioning pack, help increase efficiency and ensure the reproducibility of your results.

          Add the following for the BioPhase 8800 system:

          • BioPhase BFS capillary cartridge - 8 × 30 cm, P/N 5080121
          • BioPhase BFS capillary cartridge - 8 × 50 cm, P/N 5080123

          Add the following for the PA 800 Plus system:

          • Pre-assembled BFS capillary cartridge - 30 cm, P/N A55625

          Compatible products

          BioPhase 8800 system

          Purpose-built for biopharmaceutical scientists seeking high-quality data, enabling multiple samples to be run in parallel.

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          PA 800 Plus system

          From development throughout QC, this established system delivers high-quality data with kit-based workflows.

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          BioPhase BFS capillary cartridge

          This pre-assembled bare-fused silica 8-capillary cartridge (8 × 30 cm, P/N 5080121; 8 × 50 cm, P/N 5080123) is compatible with the DNA 20 kb Plasmid and Linear kit and the BioPhase 8800 system.

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          BFS capillary cartridge for the PA 800 Plus system

          This pre-assembled bare-fused silica capillary cartridge (P/N A55625) is compatible with the DNA 20 kb Plasmid and Linear kit and the PA 800 Plus system.

          Learn more

          Related applications

          Resources

          1 As compared to prior SCIEX technology.

          2SYBR™ is a trademark of the Life Technologies Corporation. SYBR™ Gold Nucleic Acid gel stain is not available for resale.

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