On-demand recordings
Featuring 15 expert talks and 3 Q&A roundtable discussions
SCIEX summit 2024
Highlighting scientific breakthroughs in mass spectrometry that really count
Access the summit on-demand recordings and learn from scientists and researchers from around the globe as they discuss the importance of LC-MS in fields like food and environmental testing, pharmaceutical and biopharmaceutical discovery and development and biomedical and omics research.
Access recordings from the live event.
Day one: Innovation and data insights
- Discover how to achieve high sensitivity, speed, and resilience when working with large, complex sample sets
- Learn strategies to speed up your covalent protein drug discovery
- Gain insights into accelerating the development of multiple modalities
- Understand the latest advancements in quantitative proteomics with ZT Scan DIA
- Overcome limitations with cutting-edge artificial intelligence quantitation software
Day two: Characterization and discovery
- Characterize and quantify calreticulin (CALR) arginylation using electron-activated top-down proteomics
- Achieve in-depth profiling of metabolites and lipids in biological samples
- Obtain accurate and reliable metabolite identification using electron-based fragmentation (EAD)
- Detect trace amounts of pesticide residues in food by targeted and non-targeted, IDA and SWATH approaches
- Enhance routine characterization of low-abundant sequence variant (SV) through fast and sensitive EAD-DDA
Day three: Quantitation and screening
- Enhanced sensitivity and robustness deliver stringent quantitation for veterinary drug testing for meat
- See how intuitive, compliant-ready SCIEX OS software enables pharmacokinetic parameter monitoring with ease and reliability
- Understand the challenges and best practices for oligoneucleotide analysis by LC-MS
- Discover how exploiting fast acquisition rates can be used to identify and quantify over 2250 fatty-acids lipids
- Hear about the accurate detection and quantitation of trace-lelvel PFAS compounds
Access recorded presentations from the live event.
Biomedical and omics research
Biomedical and omics researchers worldwide rely on cutting-edge LC-MS/MS solutions to meet their dynamic needs. From biomarker discovery to translational research and beyond, SCIEX technology empowers you to delve deeper into the intricacies of biology to uncover crucial insights with unparalleled precision and efficiency.
Expanding qualitative and quantitative sensitivity for immunopeptidomics using ZT Scan DIA
Tony Purcell, Professor, Monash University
Learn how utilizing a research-grade Zeno-trap time-of-flight LC-MS system can advance current applications in immunopeptidomics and proteomics, including:
- What is ZT Scan DIA, the technology and how it benefits data acquisition
- Preliminary proteomics and immunopeptidomics results using ZT Scan DIA
- Future applications of immunopeptidomics using ZT Scan DIA
Artificial intelligence quantitation (AI quant): molecular structure and mass spectral data quality driven processing of high-resolution mass spectrometry for quantitative analysis
Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert
High-resolution mass spectrometry (HRMS) has long sought to be a viable alternative for quantitative workflows but has been unable to broadly compete, mainly due to the lack of suitable data processing software. In this work we describe:
- A molecular structure-dependent workflow
- The holistic and user-independent processesing of HRMS data
- The automatic quantification of small drug-like molecules
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In-depth metabolomics and lipidomics analyses
Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz Munich
Routine measurement of metabolites and lipids has become a cornerstone of biomedical research. At the MPC, Helmholtz Munich, non-targeted analysis is based on the SCIEX ZenoTOF 7600 for accurate and in-depth analysis and annotation of metabolites and lipids from different biological specimens.
- Application of Zeno trap enabled non-targeted workflows to large scale metabolomics and lipidomics studies
- Enhanced sensitivity enables deeper profiling of biological samples
- Increased confidence in identifications through comprehensive structural elucidation using Electron activated dissociation (EAD)
Top-down characterization of post-translational arginylation using electron activated dissociation (EAD)
Tom Lin, Instructor, Washington University in St. Louis
Learn about our top-down proteomics workflow for the characterization and quantification of calreticulin (CALR) arginylation. MS1 profile and MS2 fragmentation were characterized by EAD. Preferential fragmentation at the CALR N-terminals yielded sufficient c ions facilitating precise localization of the arginylation sites. Learn about:
- Arginylation regulates CALR degradation and ER function
- EAD is efficient in characterizing N-terminal arginylation
- The workflow sets up the foundation for unbiased top-down activity-based arginylation profiling
Fast scanning quantitative lipidomics analysis using the SCIEX 7500+ system
Paul Baker, Senior Scientific Liason, Lipidomics and Metabolomics, SCIEX
A panel of >2250 lipids was used to demonstrate the power and capability of the SCIEX 7500+ system to sensitively identify and quantify lipids at the fatty acid level using a multiple reaction monitoring (MRM). HILIC-like chromatography provides class separation to mitigate inter-lipid class isobaric overlap, but means that the speed at which individual MRM transitions are measured is crucial to ensure good coverage and quantitative accuracy.
- The ~4X faster analytical speed of the SCIEX 7500+ system improves the quantitative performance of a large, broadly targeted lipid panel
- The number of lipid molecular species quantified with %CV < 20 increased 59% during a period of the highest MRM concurrency (n = 925) compared to the fastest speed recommended on the SCIEX 7500 system
- The faster MRM acquisition rates improve peak shapes by increasing the number of data points across the peak and providing better resolution of near-eluting lipid isomers
Pharma and biopharma
To bring safe and effective therapies to market, it is vital for pharma and biopharma scientists to have the right information available at every stage of the process. Discover analytical strategies that are advancing pharma and biopharma research through a commitment to innovation, and enabling comprehensive small and large molecule analysis, from discovery to commercialization.
Advancing robustness and serviceability of a high-end triple quad mass spectrometer in a high-throughput bioanalytical lab
Rathna Veeramachaneni, Director, Biopharma LC-MS/MS, KCAS Bioanalytical
Highly sensitive mass spectrometers allow the advanced bioanalytical labs of today to achieve the LOQ required of potent biologic drugs and provide maximum flexibility in choice of sample prep techniques. However robust mass spectrometers are crucial for several reasons:
- Aid in ensuring the consistent quantitative performance of an assay
- Effectively allow a lab to meet their project timelines
- Are essential for handling the inherent variability of analyzing diverse molecules and diverse samples required for bioanalysis
The new fast in drug discovery: a new MS system to study covalent protein drugs
Markus Stöckli, Senior Principal Scientist, Novartis Institutes for BioMedical Research
The combination of a super-fast sampling interface with the highly sensitive ZenoTOF 7600 system makes it an ideal system for evaluating covalent binding screens. Main findings in our experiments using the Echo® MS+ system:
- Speed: measurements from a full 384-well plate in 15 min or 1536-well plate in less than one hour
- Precision: low injection volume supports repetitive measurements and time series; capture full kinetic data set in one experiment
- Quality: high data quality allows for full automated processing, delivering immediate hitlist output
icIEF-UV/MS characterization of charge Isoforms for biotherapeutic products
Xiaoping He, Senior Scientst, Pfizer
Characterization of icIEF charge isoforms, whether in early product development or associated with licensure filings, is usually achieved through ion exchange (IEX) liquid chromatography coupled with mass spectrometry. icIEF-MS technology has recently emerged to enable the direct characterization of specific species in the icIEF electropherogram, eliminating the need for extensive method development.
- Discover opportunities to advance core platform technologies with novel characterization approaches
- Hear case studies on understanding molecular structure information
- New approaches to characterizing post-translational modifications of charge isoforms
Artificial intelligence quantitation (AI quant): molecular structure and mass spectral data quality driven processing of high-resolution mass spectrometry for quantitative analysis
Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert
High-resolution mass spectrometry (HRMS) has long sought to be a viable alternative for quantitative workflows but has been unable to broadly compete, mainly due to the lack of suitable data processing software. In this work we describe:
- A molecular structure-dependent workflow
- The holistic and user-independent processesing of HRMS data
- The automatic quantification of small drug-like molecules
MetID innovations in pharma: exploring new avenues for metabolite structure elucidation
Nari Talaty, Principal Research Scientist II, AbbVie
In DMPK development metabolite identification (metID) studies, in vitro and in vivo systems often produce heterogenous mixtures of metabolites. The analytical separation, quantitation, and accurate identification of these (phase I and II) metabolite structures is critical. The implementation of the ZenoTOF 7600 system to ADME workflows will be highlighted with emphasis on EAD fragmentation and (automated) data processing.
- Systematic evaluation of metabolites across different chemical classes by comparing conventional fragmentation modes (CID & HCD) with EAD fragmentation. Use of EAD to solve complicated chemical structures and localize biotransformations.
- In vitro workflows for Discovery ADME Applications to ID metabolic labilities.
- Incorporating mass defect filtering and isotope ratio filtering into data acquisition for in vivo ADME projects.
Reduce risk and accelerate cell line development with iCIEF-UV/MS technology
Kristen Nields, Principal Scientist, J&J Innovative Medicines
Guide decisions during cell line development with the Intabio ZT system with more information on how process changes affect cell line and product stability.
- How to utilize icIEF-UV/MS to identify sequence variants
- How this information can be used to create a mitigation strategy
- What more information in less time means to groups throughout process development
Maximizing analytical potential and navigating changes in the validated laboratory with SCIEX OS software
April Quinn-Paquet, Global Marketing Product Manager, Software, SCIEX
Having the right information available at every stage of the process is vital to bringing safe and effective therapies to market.. SCIEX OS software is a vital component of this process, from discovery to development. See how:
- Your pharma lab workflows can benefit from SCIEX OS software
- Central Admin Console enables comprehensive audit configurations for full traceability
- Professional services are available to assist in migrating from legacy Analyst software and change control support plansis
Approaches for the bioanalytical analysis of oligonucleotide therapies
Shane Karnik, Senior Laboratory Director, Aliri Bioanalysis
Oligonucleotide therapeutics are on the rise in drug development and regulatory submissions. These drugs face challenges with bioanalytical extraction and analytical detection due to their chemical backbone or other stability-enhancing modifications.
- Learn about bioanalytical challenges and best practices for oligonucleotide analysis
- Hear case studies in the quantitation of oligonucleotide therapies using different mass spectrometry platforms
- Gain insights into improved method development for obtaining validated assays for regulatory evaluations using high-resolution mass spectrometry in early-stage development
Food and environmental
From routine applications such as mycotoxins and contaminant quantitation, to trace-level PFAS detection in complex matrices, our food and environmental customers and scientists around the globe are empowered by the combination of sensitive hardware, flexible software, and industrial expertise to enable testing capabilities for comprehensive data collection and report generation.
Artificial intelligence quantitation (AI quant): molecular structure and mass spectral data quality driven processing of high-resolution mass spectrometry for quantitative analysis
Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert
High-resolution mass spectrometry (HRMS) has long sought to be a viable alternative for quantitative workflows but has been unable to broadly compete, mainly due to the lack of suitable data processing software. In this work we describe:
- A molecular structure-dependent workflow
- The holistic and user-independent processesing of HRMS data
- The automatic quantification of small drug-like molecules
Time-of-flight mass spectrometry applications in food science: targeted and non-targeted approaches
Francisco José Díaz-Galiano, Postdoctoral researcher, University of Almería
Time-of-flight mass spectrometry has become a key tool in analytical laboratories involved in food analyses. This instrumentation can be used for both targeted and non-targeted approaches. In this presentation, we will discuss:
- Overview of practical targeted and non-targeted approaches in food analysis
- Application of a SWATH® approach to the analysis of pesticide residues in food
- Application of IDA approaches in non-targeted food analyses
Veterinary drug analysis: SCIEX 7500 system vs 7500+ system
Claudia Ancillotti, Method Development Manager, Biochemie Lab
Biochemie Lab shares their expertise in veterinary drug analysis. With over 25 years of experience and strict adherence to Commission Regulation (EU) No 37/2010, Biochemie Lab processes more than 100,000 samples annually, providing comprehensive food and environmental testing.
- Learn how Biochemie Lab conducts veterinary drug testing with specialized veterinarians, covering a wide range of analyses, including residual veterinary drugs, anabolic substances, and microbiological tests.
- Discover how they overcome challenges in analyzing complex animal-origin matrices like meat and offal using the SCIEX QTRAP 7500 system.
- Explore their proficiency testing, method validation results, and ask questions directly from leading experts in the field.
From sample collection to data processing - PFAS program at State Hygienic Lab
Majid Nada Environmental Lab Scientist, State Hygienic Laboratory at the University of Iowa
The State Hygienic Lab at University of Iowa had the PFAS program running for couple of years and is accredited and certified by the state, EPA, and UCMR5 program to test for PFAS in water samples. However, testing encompasses different environmental and biological matrices. This presentation will look at the program and focus on:
- Approaches from sample collection to data processing and reporting.
- Testing for PFAS in water matrices (drinking, raw, well, surface, and groundwater) based on EPA 537.1 and 533 PFAS methods.
- New method for PFAS in biological samples.
Access recorded presentations from the live event.
Q&A roundtables
You asked, our experts share their research, insights and ideas.. Hear from panelists expexploring the latest technological advancements that are transforming quantitative research.
Day one: Innovation and data insights
Moderated by: Jamie Wighton, Senior Director LC-MS Research, SCIEX
Panelists included:
- Rathna Veeramachaneni, KCAS Bio
- Xiaoping He, Pfizer
- Markus Stöckli, Novartis Institutes for BioMedical Research
- Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert
Day two: A deeper look at high-resolution mass spectrometry
Moderated by: Jose Castro-Perez, Vice President of Product Management, SCIEX
Panelists included:
- Tom Lin, Washington University in St. Louis
- Michael Witting, Helmholtz Munich
- Francisco José Díaz-Galiano, University of Almería
- Kirsten Nields, J&J Innovative Medicines
- Tatjana Talamantes, SCIEX
Day three: Applications of high-quality quantitation and screening workflows
Moderated by: Ian Moore, Sr. Technical Product Manager, Nominal Mass, SCIEX
Panelists included:
- Roberto Riccio, Biochemie Lab
- Shane Karnik, Aliri Bioanalysis
- April Quinn-Paquet, SCIEX
- Paul Baker, SCIEX
- Majid Nada, State Hygienic Laboratory at the University of Iowa
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