Set your own schedule for CRISPR/Cas9 gene editing with innovative, intuitive analytical solutions.
Unleash the potential of your Cas9 messenger RNA (mRNA), single guide RNA (sgRNA) and Cas9 proteins by understanding quality, purity and safety and assessing on/off target effects.
Explore the latest and trending topics
The development of novel gene-editing approaches goes beyond the treatment of diseases or their symptoms. However, analytical solutions for CRISPR/Cas9 analysis need to keep up with the demands of these new approa
Leverage highly sensitive techniques for characterizing large synthetic guide RNAs and in vitro transcribed RNA products, and understand low-abundance post-translational modifications (PTMs) of Cas9 proteins and CRISPR/Cas9 effects on the proteome. With dedicated analytical solutions, personalized medicine is only a step away.
Workflow
The latest gene-editing approaches require overcoming new analytical challenges. Confirmation of the size, integrity and purity of large synthetic guiding RNAs (sgRNA and pegRNA) and confident analysis of Cas9 mRNA require the highest data quality over a wide size range.
Move past analytical challenges with high separation quality, sensitivity and superior reproducibility. Reclaim your time with intuitive software and kit-based workflows that are compatible with compliant data systems.
Suited for:
Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.
Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.
Pre-assembled bare-fused silica 8-capillary cartridge for the BioPhase 8800 system.
Featured resources
Technical note
High-resolution size and purity determination of sgRNA and Cas9 mRNA in a single analysis
Learn about an intuitive kit-based solution for the characterization of mRNA integrity and purity. Break through boundaries with workflows that provide the highest resolution for sgRNA and mRNA in a single analysis.
Technical note
Compliant-ready, streamlined data management for RNA analyses with the Empower CDS
Set your own schedule for mRNA analyses by streamlining your CE data management with the Empower CDS.
Webinar
Empowering next-generation therapy development with streamlined data integration
Discover how to save time by streamlining your CE data management with the Empower CDS.
Suited for:
Characterize therapeutic molecules with confidence with the kit-based system.
Purity and integrity analysis of RNA therapeutics, vaccines and single stranded oligos.
Pre-assembled bare-fused silica capillary cartridge for the PA 800 Plus system.
Workflow
A deeper understanding of gene-editing mechanisms paves the way for higher target specificity and minimized side effects. Engineering Cas9 proteins with improved binding and stability can be one approach. However, ensuring the correct function requires comprehensive characterization of the Cas9 proteins.
Obtain high-quality, comprehensive sequence and fragment coverage with advanced MS/MS sensitivity. Explore how to elucidate the position and identity of amino acid substitutions/isomers and other challenging PTMs with confidence.
Suited for:
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.
Unleash the analytical power of the next-generation software platform for data acquisition and processing.
Suited for:
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
A purpose-built QTOF system, designed specifically to accelerate everyday biologics characterization.
Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.
Unleash the analytical power of the next-generation software platform for data acquisition and processing.
Workflow
More sophisticated, chemically synthesized gRNAs are in development with the goal of increasing efficiency and specificity of CRISPR/Cas9 gene editing. One of these newly emerging classes is pegRNA with sizes ranging from around 120–250 nucleotides (nt).
The length and associated risk of secondary structure formation, however, pose challenges for the analytical assessment of its purity.
Move beyond these boundaries of CRISPR/Cas9 analysis and confirm size and purity for large pegRNA with confidence. Rely on high resolving power and repeatability to separate your products from impurities with similar properties.
Characterize therapeutic molecules with confidence with the kit-based system.
Kit with coated capillaries, gel, and standards to analyze single-stranded nucleic acids on the PA 800 Plus system.
Workflow
Oligonucleotides—such as guide RNAs, sgRNAs and pegRNAs—are prone to a high number of impurities due to their stepwise chemical synthesis. Obtaining molecular weight information to confirm the identity of the main product and quantitation of related impurities is key for quality assessment prior to performing lengthy cell-based assays.
Take control of the purity of your products with high-quality, accurate mass data derived from intuitive solutions. Enable your team to gain key insights that are relevant for ensuring safety and efficacy.
Suited for:
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
A purpose-built QTOF system, designed specifically to accelerate everyday biologics characterization.
Unleash the analytical power of the next-generation software platform for data acquisition and processing.
Suited for:
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
Unleash the analytical power of the next-generation software platform for data acquisition and processing.
Workflow
Despite efforts to increase the specificity and efficiency of CRISPR/Cas9 gene editing, unexpected effects on the proteome can occur. Biochemistry-based assays are blind towards such changes, or they only provide limited information on affected proteins.
Give your team the advantage of comprehensive unbiased characterization of proteome changes to streamline the development of gene-editing tools. Reach new milestones with the dynamic range, sensitivity and reproducibility needed to better understand on- and off-target effects of gene therapies while enabling simultaneous quantitation in samples with limited amounts.
Enjoy the perfect balance of sensitivity and robustness with a feature-rich microflow LC solution built for small and large molecule quantitation and characterization.
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
Unleash the analytical power of the next-generation software platform for data acquisition and processing.
Featured resources
Article
Defying uncertainty in gene therapy
Clearly understand CRISPR/Cas9 effects on the proteome. Leverage high-quality MS/MS spectral data to identify even low-abundant, unexpected changes that ligand-binding assays might miss.
Webinar
Characterization of off- and on-targets of CRISPR/Cas9 gene editing
Master the development of gene-editing approaches with the ability to confidently detect proteome changes that are often missed by traditional assays. Access the highest quality spectral data to ensure the safety and efficacy of your next-generation therapies.
Article
Defying uncertainty in gene therapy
Clearly understand CRISPR/Cas9 effects on the proteome. Leverage high-quality MS/MS spectral data to identify even low-abundant, unexpected changes that ligand-binding assays might miss.
Webinar
Characterization of off- and on-targets of CRISPR/Cas9 gene editin
Master the development of gene-editing approaches with the ability to confidently detect proteome changes that are often missed by traditional assays. Access the highest quality spectral data to ensure the safety and efficacy of your next-generation therapies.
Technical note
Comprehensive method development for a wide size range of single-stranded nucleic acids
Create pathways for effective method development and learn how to achieve the highest quality data for RNA products with sizes ranging from 50–9,000 nucleotides and beyond.
Technical note
Characterization of amino acid substitutions and post-translational modifications (PTMs) of an engineered Cas9 protein
Unleash the potential of protein characterization with novel fragmentation solutions. Explore paths to understanding complex PTMs of your engineered Cas9 proteins with unprecedented depth.
Technical note
Analysis of pegRNA
Overcome secondary structure challenges presented by large-sized oligonucleotides for gene editing. Learn how to enable accurate and easy purity analysis of complex synthetic pegRNAs.
Technical note
Molecular weight confirmation of large synthetic oligonucleotides and their impurities
Imagine taking control of your CRISPR/Cas9 gene-editing systems by ensuring sgRNA integrity and purity. Be empowered to take on the challenge of future medicine development with accurate mass solutions that provide meaningful answers.
Technical note
High-resolution size and purity determination of sgRNA and Cas9 mRNA in a single analysis
Learn about an intuitive kit-based solution for the characterization of mRNA integrity and purity. Break through boundaries with workflows that provide the highest resolution for sgRNA and mRNA in a single analysis.
Technical note
Compliant-ready, streamlined data management for RNA analyses with the Empower CDS
Set your own schedule for mRNA analyses by streamlining your CE data management with the Empower CDS.
Webinar
Empowering next-generation therapy development with streamlined data integration
Discover how to save time by streamlining your CE data management with the Empower CDS.
Expand the possibilities of drug delivery with innovative analytical solutions for lipid raw materials, LNPs and other non-viral carriers. Move beyond boundaries in structural elucidation and monitoring of analytes to estabilish confidence in the quality and stablity of your products.
Take control of the unknowns. Gain confident sequence confirmation and localization of post-translational modifications (PTMs) at the sub-unit level.