Take control of the unknowns. Enable confident sequence confirmation and localization of post-translational modifications (PTMs) at the sub-unit level. Leverage high-quality data to develop routine and reproducible workflows from discovery through commercialization.
Subunit mass analysis and middle down
Explore the latest and trending topics
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Video
Time-saving strategies for subunit mass analysis
Minimize sample preparation and provide in-depth information on critical quality attributes for scientists in early development. Learn more in this webinar.
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Webinar
Gain critical PTM information without peptide mapping
Guide decisions in early development and gain a comprehensive molecular profile and structural confirmation of engineered disulfides, at the subunit level.
Overview
Keep with the pace of next generation drug development.
Subunit mass analysis can be used as a screening tool for candidate developability, identity and quality. This approach addresses the need for fast and intuitive characterization of therapeutic proteins in early development to not only ensure drug efficacy and safety, but to quickly distinguish post-translational modifications (PTMs) and drug conjugation sites.
Novel, time-saving strategies for monoclonal antibody (mAb) subunit protein analysis provide in-depth PTM information and leverage high-quality data to develop routine and reproducible workflows from discovery through commercialization.
Rapid middle-down protein analysis
Workflow
Make decisions on candidate developability or product quality sooner.
A challenge in rapid characterization of therapeutics is obtaining high sequence coverage and in-depth information on PTM localization while minimizing sample preparation artifacts like undesired modifications or loss of information on complex therapeutics like antibody drug conjugates.
Subunit mass analysis strategies – such as middle down mAb fragment analysis, can be employed to address these challenges.
A single-injection, EAD-based, middle-down workflow for subunit mass analysis leverages information-rich and reproducible EAD fragmentation with automated data analysis to consistently deliver high sequence coverage of mAb subunits and enable confident sequence and PTM confirmations.
- Save time with confident high sequence coverage of mAb fragments in one injection
- Achieve in-depth analysis with localization of common PTMs like glycosylation and oxidation
- Improve productivity with minimal method development simple software workflows
Resources
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Technical note
Consistently achieve up to 80% sequence coverage of mAb subunits
A middle-down strategy for routine subunit mass analysis with high sequence coverage and localization of PTMs, such as glycosylation and oxidation
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Technical note
Identify mass neutral PTMs and achieve high sequence coverage
Confident sequence confirmation and accurate localization of glycosylation of a complex tri-specific antibody
Suited for:
- In-depth characterization of mAb subunits
- Rapid PTM localization
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
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ZenoTOF 7600 system
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
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Biologics Explorer software
Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.
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All resources
Filter:
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Technical note
Tackle characterization of complex glycoform distributions
Obtain detailed information about glycosylation at the subunit level with straightforward, visual data interpretation and accurate peak assignment.
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Webinar
Time-saving strategies for subunit analysis
Minimize sample preparation and provide in-depth information on critical quality attributes for scientists in early devleopment. Learn more in this webinar.
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Technical note
The best of both worlds: What is the EAD-based middle-down workflow?
The streamlined EAD-based middle-down workflow enables confident sequence confirmation and PTM analysis in a single injection.
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Document
Gain deeper information on complex protein therapeutics
Learn more about the power of EAD for characterization of large biomolecules.
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Technical note
Increase confidence in rapid sequence confirmation and localization of PTMS
Enable comparative middle-down analysis for the characterization of biosimilars or sequence variants with visually intuitive data analysis tools.
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Technical note
Consistently achieve up to 80% sequence coverage of mAb subunits
A middle-down strategy for routine subunit mass analysis with high sequence coverage and localization of PTMs, such as glycosylation and oxidation.
-
Technical note
Identify mass neutral PTMs and achieve high sequence coverage
Confident sequence confirmation and accurate localization of glycosylation of a complex tri-specific antibody.
Application areas
Charge heterogeneity analysis
Pursue the end goal with confidence. Achieve the full picture and accelerate decisions with comprehensive characterization of biopharmaceutical charge variants.
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Intact protein analysis
Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.
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