Obtain high resolution glycan data for released glycan profiling.
Glycoprotein to identified glycans
Fast glycan labeling and analysis kits
Confident N-glycan profiling with capillary electrophoresis
Obtain high resolution glycan data and rapidly identify glycan heterogeneity, including sialylated N-glycans.
Simplified sample preparation, rapid separations and automated glycan identification provide the information needed to make confident decisions quickly.
Enable the characterization of more samples for comparability assessments or minimize the time needed to course correct processes from development through to commercialization.
For use on the PA 800 Plus system and the BioPhase 8800 system
Analytical throughput needs change during drug development. Fast glycan labeling and analysis kits are available for both the single capillary PA800 Plus system and the multi-capillary BioPhase 8800 system.
- Faster than HILIC: Perform rapid glycan separation while retaining high resolution information on n-glycan profiles
- Easy sample preparation: Sample clean up with magnetic-bead technology enable sample preparation in just an hour
- Automation friendly: Magnetic bead technology eliminates the need for ultra-centrifugation or advanced pipetting techniques
Validated kits for glycan profiling
Fast glycan labeling and analysis kit for the PA800 Plus system
The fast glycan labeling and analysis kit is designed for use on the PA 800 Plus system and is used to rapidly label glycoprotein released N-Glycans with APTS followed by the effective removal of the excess dye using a magnetic bead-based clean-up.
The labeled glycans are separated by high-resolution capillary electrophoresis utilizing laser-induced fluorescence (LIF) detection and the HR-NCHO separation gel. Glycan identification is determined based on their normalized electrophoretic mobility referenced against pre-determined GU values using the appropriate bracketing standard.
This kit provides reagent volumes for analysis of 100 samples
BioPhase fast glycan labeling and analysis kit
The BioPhase fast glycan labeling and analysis kit enables comprehensive, high-resolution characterization of glycosylated biotherapeutics on a diverse array of N-glycan species while maintaining screening throughput.
Simplified sample preparation with magnetic bead technology combines with rapid separation on 8 parallel capillaries to enable glycan analysis at a rate of 1 minute per sample.
This kit provides reagent volumes for analysis of 200 samples.
Compatible products
BioPhase 8800 system
Purpose-built for biopharmaceutical scientists seeking high-quality data, enabling multiple samples to be run in parallel.
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PA 800 Plus system
From development throughout QC, this established system delivers high-quality data with kit-based workflows.
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BioPhase BFS capillary cartridge
This pre-assembled bare-fused silica 8-capillary cartridge (8 × 30 cm, P/N 5080121; 8 × 50 cm, P/N 5080123) is compatible with the DNA 20 kb Plasmid and Linear kit and the BioPhase 8800 system.
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BFS capillary cartridge for the PA 800 Plus system
This pre-assembled bare-fused silica capillary cartridge (P/N A55625) is compatible with the DNA 20 kb Plasmid and Linear kit and the PA 800 Plus system.
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Related applications
Cell line analysis
Set the pace with confidence. Cell line monitoring and analysis solutions verify the identity, purity and stability of lead candidates with high throughput strategies that are ready for automation.
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Glycan analysis
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ADC analysis
Be clear on what you have and how much. Understand ADC drug loading profiles regardless of the inherent heterogeneity and high molecular weight of these molecules.
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Resources
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Technical note
Automated and immediate glycan identification
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Technical note
Reproducibility study of a high throughput release N-glycan workflows
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Technical note
Automate released n-glycan analysis on the Biomek i5 liquid handler
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Technical note
High-throughput released N-glycan identification using CE
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Webinar
High-throughput released N-glycan identification using CE
The SCIEX Fast Glycan Analysis Kit, (Part Number B94499PTO) includes sample preparation beads, magnet separator, labeling reagent, separation gel reagent, internal GU standard and pre-assembled cartridge (part number A55625) for your convenience and ease-of-use.*
* Sodium Cyanoborohydride and PNGase F not included.