Overview
Cell-line monitoring throughout drug development helps ensure quality in the biotherapeutic.
Gain the advantage of knowledge up front, with rapid, sensitive, and accurate cell line analysis strategies.
Capillary electrophoresis (CE) and mass spectrometry (MS) workflows address the need for speed, while providing accurate, quantitative information on cell culture media components and cell line stability.
Workflow
Fast cell-line screening with CE
Charge ahead to find candidates that make it to market.
Characterize critical properties, address throughput challenges and gain valuable quantitative data on cell-line samples with automation-friendly capillary electrophoresis (CE) workflows.
High-throughput purity, charge heterogeneity and N-glycan analysis CE workflows provide speed, reproducibility and resolution for product characterization during process and cell-line development.
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Enable quick, easy assessment of product quality
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Minimize sample preparation and method development
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Work toward automation with simple to integrate multi-channel formats
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Software templates optimized for peptide mapping and PQA selection
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Reduce regulatory risk with direct control through the Empower Chromatography Data System
Fast cell-line screening with CE
Solution
BioPhase 8800 system
BioPhase CE-SDS Protein Analysis kit
Fast cell-line screening with CE
Solution
- High-throughput charge heterogeneity analysis
- Rapid CE-SDS assays
BioPhase 8800 system
BioPhase Capillary Isoelectric Focusing (cIEF) kit
Fast cell-line screening with CE
Solution
- High throughput N-linked glycan analysis
- Rapid glycan expression profiling
BioPhase 8800 system
BioPhase Fast Glycan Labeling and Analysis kit
Workflow
Monitoring of target CCM compounds with LC/MS
Gain qualitative understanding of cell culture media (CCM) components.
Comprehensive information on CCM components sets the stage for success by helping to ensure optimal CCM conditions and the overall quality of the biotherapeutic.
LC-MS workflows designed to address the wide range of polarities and separation properties of CCM compounds provide a highly sensitive method to monitor target analytes in a single run.
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Improve detection and quantitation limits of low-level analytes
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Save sample and reduce matrix effect with less injection volume
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Identification and quantitation of over 110 compounds in a single run
Monitoring of target CCM compounds with LC/MS
Solution
- High-throughput monitoring of CCM compounds
- Identification of targeted CCM compounds
ExionLC AE system
SCIEX 7500 system
SCIEX OS software
All resources
Assess molecular liabilities more quickly with the BioPhase 8800 system, allowing rapid developability assessment to streamline the selection of candidates that can progress into an effective therapy
Enable reproducible separation of a 96-well plate in under 2 hours.
Significantly improve throughput and process 96-well plates in 12 hours with reproducible separation between instruments and kits.
Streamlined N-linked glycan analysis that employs an automated sample preparation with a magnetic bead-mediated glycan workflow followed by capillary electrophoresis (CE).
Discuss high-throughput strategies for glycan analysis to condense development timelines.
Screen a large number of clones for lead clone selection with an automation friendly multi-capillary CE-SDS workflow.
Streamline evaluation of multiple factors and post-translational modifications in a single set of experiments with a multifactorial DoE approach
High-throughput glycan expression profiling with automation friendly sample preparation and labeling.
Improve detection of low-level target analytes with a single method
Associated applications
Charge heterogeneity analysis
Pursue the end goal with confidence. Achieve the full picture and accelerate decisions with comprehensive characterization of biopharmaceutical charge variants.
Glycan analysis
Fully understand drug candidate glycoprofiles with pipeline-specific solutions. Gather critical high-resolution information and site-specific data points with advanced glycan analysis for complex biologic development.