ADC analysis

Be clear on what you have and how much.

Understand ADC drug loading profiles regardless of the inherent heterogeneity and high molecular weight of these molecules. 

Overview

Up the game for ADC analysis

ADC analysis strategies tackle the characterization of the more structurally complex antibody drug conjugates (ADC) that are providing the next level of therapeutics to combat specific conditions.
These molecules have greater heterogeneity compared to unconjugated proteins and have a variable number of linkers and payloads. This enhances the number of proteoforms, making characterization more challenging.

Take control of the information with MS and CE based solutions designed to address the complexity of these next generation therapeutics with increased speed and detail.

In-depth ADC characterization

Dig into the complex structure of an ADC​.

Ensuring the quality, safety, and efficacy of antibody-drug conjugates (ADCs) requires comprehensive characterization of protein sequences, drug payloads, and post-translational modifications (PTMs). Traditional collision-based MS/MS techniques often fail to preserve labile modifications and can cause extensive fragmentation, leading to the loss of valuable site-specific information.​

With a signature fragmentation pattern, electron-activated dissociation (EAD)-based workflows allow for in-depth ADC characterization. ​

Discover LC-MS workflows that can harness the power of EAD for in-depth characterization of ADCs.

  • High sequence coverage with single-injection methods​
  • Precise localization of drug payloads​
  • Accurate drug-antibody ratio determination​
  • Clear differentiation of challenging isomers​

Comprehensive characterization of challenging ADCs​

Suited for:

  • Precise localization of drug payload​s
  • Clear determination of PTMs​
  • Need for rapid high-sequence coverage
ExionLC AE system

Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.

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ZenoTOF 7600 system

A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.

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Biologics Explorer software

Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.

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Intact ADC characterization

Untangle the charge heterogeneity information of an intact ADC.

Charge heterogeneity is present in most biopharmaceutical protein products. For ADCs, the antibody, linker and payload all contribute to the heterogeneity, adding complexity to the charge variant profiles. 

An integrated icIEF-UV/MS workflow enables the simple determination of charge variant profiles while reducing resources and accelerating development timelines. Determine what is driving the change in the charge profile–the payload, the linker or the monoclonal antibody (mAb).  

  • Reduce charge heterogeneity analysis workflow from weeks to minutes
  • Elucidate critical post-translational modifications (PTMs) including glycosylation, deamidation and linker modifications
  • Enable mass identification of charge variants and modifications on intact ADCs
  • Monitor charged modifications on ADC payload in near real-time

Intact ADC characterization

Suited for:

  • Intact protein analysis of ADC molecules
  • Real-time monitoring of ADC payload modifications
  • In-depth characterization of PTMs on the ADC mAb
Intabio ZT system

Eliminate the guess work and monitor biologics at the intact-protein level, gaining insights into charge variant-associated quality attributes.

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Biologics Explorer software

Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.

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Drug antibody ratio monitoring

Workflow

Take control of information on ADC quality. Drug antibody ratio (DAR) information is essential for assessing the quality of ADCs. Understand ADC drug loading profiles with high throughput and intuitive LC/MS and CE solutions for DAR monitoring at the intact protein level.

  • Complete solution for acquisition and tracking of DAR changes
  • High-throughput DAR monitoring for large sample sets
  • DAR quantitation and monitoring on compliance-ready software

Comprehensive drug antibody ratio monitoring

Suited for:

  • Routine monitoring of DAR for ADCs
  • Comprehensive assessment of DAR
ExionLC AE system

Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.

Learn more
ZenoTOF 7600 system

A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.

Learn more
Biologics Explorer software

Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.

Learn more

Routine drug antibody ratio monitoring

Suited for:

  • Routine monitoring of DAR for ADCs
  • Targeted assessment of DAR
ExionLC AE system

Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.

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X500B QTOF system

A purpose-built QTOF system, designed specifically to accelerate everyday biologics characterization.

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SCIEX OS software

Unleash the analytical power of the next-generation software platform for data acquisition and processing.

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CE solutions for DAR calculations

Suited for:

  • Calculating DAR ratios with CE
PA 800 Plus system

Characterize therapeutic molecules with confidence with the kit-based system.

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SDS-MW Protein Analysis kit for the PA 800 Plus system

Effective quantification and determination of protein purity and size.

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Characterization of ADC raw material

Workflow

Build on a strong backbone.
ADCs are complex molecules consisting of an antibody chemically tethered to a drug payload. While the complexity of the association is important to understand, the raw material itself is also fundamental to the success of the method of action and requires characterization. Accelerate ADC analysis with characterization of raw materials using high-throughput CE-based workflows.

  • High-throughput assays for purity, charge heterogeneity and N-linked glycans
  • Reduce method development time with parallel processing
  • Optimize separation conditions for complex ADC development

Optimize separation conditions for charge heterogeneity and purity assessment

Suited for:

  • Method development for cIEF and CE-SDS assays
  • High-throughput purity analysis​
  • Rapid cIEF screening
BioPhase 8800 system

Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.

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BioPhase CE-SDS Protein Analysis kit

Effective quantification and determination of protein purity and size.

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BioPhase Capillary Isoelectric Focusing (cIEF) kit

Experimental protein isoelectric focusing point (pI) and analysis of charge variants.

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Rapid assessment of protein purity

Suited for:

  • High-throughput purity analysis
  • Rapid CE-SDS assay
BioPhase 8800 system

Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.

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BioPhase CE-SDS Protein Analysis kit

Effective quantification and determination of protein purity and size.

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Rapid assessment of protein purity

Suited for:

  • Protein purity assessment in QC
PA 800 Plus system

Characterize therapeutic molecules with confidence with the kit-based system.

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SDS-MW Protein Analysis kit for the PA 800 Plus system

Effective quantification and determination of protein purity and size.

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Charge heterogeneity assessment with cIEF

Suited for:

  • High-throughput charge heterogeneity analysis
  • Rapid charge profile monitoring during development
BioPhase 8800 system

Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.

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BioPhase Capillary Isoelectric Focusing (cIEF) kit

Experimental protein isoelectric focusing point (pI) and analysis of charge variants.

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Charge heterogeneity assessment with CZE

Suited for:​

  • Native charge heterogeneity assessment with CZE​
  • Charge heterogeneity assessment within QC
PA 800 Plus system

Characterize therapeutic molecules with confidence with the kit-based system.

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CZE Rapid Charge Variant Analysis kit

Native state charge variant analysis

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Associated applications

Intact protein analysis

Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.

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Subunit mass analysis and middle-down

Take control of the unknowns. Gain confident sequence confirmation and localization of post-translational modifications (PTMs) at the sub-unit level.

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Peptide analysis

Achieve new heights in PTM analysis with confidence and speed. Define critical quality attributes (CQAs) and streamline processes from early to late-stage development with in-depth peptide mapping solutions for next-generation protein therapeutics and standard monoclonal antibodies (mAbs).

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