Be clear on what you have and how much.
Understand ADC drug loading profiles regardless of the inherent heterogeneity and high molecular weight of these molecules.
ADC analysis
Explore the latest and trending topics
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Technical note
Gain in-depth and comprehensive characterization of a new-generation Cys-conjugated ADC
A full characterization workflow of a complex ADC. EAD enabled peptide mapping provides confident identification of T-DXd peptides with partial or complete payload conjugation for accurate DAR determination and impurity assessment.
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Hub
Deep dive into the characterization of an antibody drug conjugate
Up your game for ADC analysis with innovative analytical strategies presented by leaders in the field.
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Technical note
Leverage the increased sensitivity of the ZenoTOF 8600 to improve intact protein analysis
Discover how the advantages of increased MS sensitivity for Intact analysis of protein therapeutics, with a workflow to provide better peak separation of glycoforms, and detection of low abundant impurities.
Overview
Up the game for ADC analysis
ADC analysis strategies tackle the characterization of the more structurally complex antibody drug conjugates (ADC) that are providing the next level of therapeutics to combat specific conditions.
These molecules have greater heterogeneity compared to unconjugated proteins and have a variable number of linkers and payloads. This enhances the number of proteoforms, making characterization more challenging.
Take control of the information with MS and CE based solutions designed to address the complexity of these next generation therapeutics with increased speed and detail.
In-depth ADC characterization
Dig into the complex structure of an ADC.
Ensuring the quality, safety, and efficacy of antibody-drug conjugates (ADCs) requires comprehensive characterization of protein sequences, drug payloads, and post-translational modifications (PTMs). Traditional collision-based MS/MS techniques often fail to preserve labile modifications and can cause extensive fragmentation, leading to the loss of valuable site-specific information.
With a signature fragmentation pattern, electron-activated dissociation (EAD)-based workflows allow for in-depth ADC characterization.
Discover LC-MS workflows that can harness the power of EAD for in-depth characterization of ADCs.
- High sequence coverage with single-injection methods
- Precise localization of drug payloads
- Accurate drug-antibody ratio determination
- Clear differentiation of challenging isomers
Comprehensive characterization of challenging ADCs
Suited for:
- Precise localization of drug payloads
- Clear determination of PTMs
- Need for rapid high-sequence coverage
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
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ZenoTOF 7600 system
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
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Biologics Explorer software
Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.
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Intact ADC characterization
Untangle the charge heterogeneity information of an intact ADC.
Charge heterogeneity is present in most biopharmaceutical protein products. For ADCs, the antibody, linker and payload all contribute to the heterogeneity, adding complexity to the charge variant profiles.
An integrated icIEF-UV/MS workflow enables the simple determination of charge variant profiles while reducing resources and accelerating development timelines. Determine what is driving the change in the charge profile–the payload, the linker or the monoclonal antibody (mAb).
- Reduce charge heterogeneity analysis workflow from weeks to minutes
- Elucidate critical post-translational modifications (PTMs) including glycosylation, deamidation and linker modifications
- Enable mass identification of charge variants and modifications on intact ADCs
- Monitor charged modifications on ADC payload in near real-time
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Webinar
Enable mass identification of charge variants and modifications on intact ADCs
Reduce charge heterogeneity analysis from weeks to minutes. Identify and monitor the charged modifications on ADC payloads in near real-time.
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Technical note
Enhance PQA identification during ADC development with confident proteoform ID, and payload localization.
Gain a level of characterization beyond traditional LC separation and CID based workflows to provide critical information to guide analytical strategies throughout ADC development.
Intact ADC characterization
Suited for:
- Intact protein analysis of ADC molecules
- Real-time monitoring of ADC payload modifications
- In-depth characterization of PTMs on the ADC mAb
Intabio ZT system
Eliminate the guess work and monitor biologics at the intact-protein level, gaining insights into charge variant-associated quality attributes.
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Biologics Explorer software
Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.
Learn more
Drug antibody ratio monitoring
Workflow
Take control of information on ADC quality. Drug antibody ratio (DAR) information is essential for assessing the quality of ADCs. Understand ADC drug loading profiles with high throughput and intuitive LC/MS and CE solutions for DAR monitoring at the intact protein level.
- Complete solution for acquisition and tracking of DAR changes
- High-throughput DAR monitoring for large sample sets
- DAR quantitation and monitoring on compliance-ready software
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Technical note
Enable faster decision making with high-throughput and intuitive DAR monitoring for ADCs
Streamlined LC-MS based workflows, with user-friendly data analysis software to enable attribute monitoring and informed decision making during process development.
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Technical note
Automate DAR calculation at the intact level
Ensure product quality and make ADC analysis accessible with a visually intuitive workflow for routine ADC characterization.
Comprehensive drug antibody ratio monitoring
Suited for:
- Routine monitoring of DAR for ADCs
- Comprehensive assessment of DAR
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
Learn more
ZenoTOF 7600 system
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.
Learn more
Biologics Explorer software
Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.
Learn more
Routine drug antibody ratio monitoring
Suited for:
- Routine monitoring of DAR for ADCs
- Targeted assessment of DAR
ExionLC AE system
Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.
Learn more
X500B QTOF system
A purpose-built QTOF system, designed specifically to accelerate everyday biologics characterization.
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SCIEX OS software
Unleash the analytical power of the next-generation software platform for data acquisition and processing.
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PA 800 Plus system
Characterize therapeutic molecules with confidence with the kit-based system.
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SDS-MW Protein Analysis kit for the PA 800 Plus system
Effective quantification and determination of protein purity and size.
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Characterization of ADC raw material
Workflow
Build on a strong backbone.
ADCs are complex molecules consisting of an antibody chemically tethered to a drug payload. While the complexity of the association is important to understand, the raw material itself is also fundamental to the success of the method of action and requires characterization. Accelerate ADC analysis with characterization of raw materials using high-throughput CE-based workflows.
- High-throughput assays for purity, charge heterogeneity and N-linked glycans
- Reduce method development time with parallel processing
- Optimize separation conditions for complex ADC development
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Technical note
Eliminate the bottleneck for drug purity analysis with high-throughput rapid CE-SDS purity analysis
Screen a large number of clones for lead clone selection with automation friendly mulit-capillary CE-SDS workflow.
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Technical note
Accelerate cIEF method development from days to hours
Streamline evaluation of multiple factors in a single set of experiments with a multifactorial DoE approach.
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Technical note
Analytical characterization of the antibody drug conjugate Enhertu using multi-capillary electrophoresis
Optimize separation conditions for charge heterogeneity and purity assessment
Suited for:
- Method development for cIEF and CE-SDS assays
- High-throughput purity analysis
- Rapid cIEF screening
BioPhase 8800 system
Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.
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BioPhase CE-SDS Protein Analysis kit
Effective quantification and determination of protein purity and size.
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BioPhase Capillary Isoelectric Focusing (cIEF) kit
Experimental protein isoelectric focusing point (pI) and analysis of charge variants.
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Rapid assessment of protein purity
Suited for:
- High-throughput purity analysis
- Rapid CE-SDS assay
BioPhase 8800 system
Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.
Learn more
BioPhase CE-SDS Protein Analysis kit
Effective quantification and determination of protein purity and size.
Learn more
PA 800 Plus system
Characterize therapeutic molecules with confidence with the kit-based system.
Learn more
SDS-MW Protein Analysis kit for the PA 800 Plus system
Effective quantification and determination of protein purity and size.
Learn more
Charge heterogeneity assessment with cIEF
Suited for:
- High-throughput charge heterogeneity analysis
- Rapid charge profile monitoring during development
BioPhase 8800 system
Purpose-built for the biopharmaceutical scientist for efficiency and quality, enabling multiple samples to be run in parallel.
Learn more
BioPhase Capillary Isoelectric Focusing (cIEF) kit
Experimental protein isoelectric focusing point (pI) and analysis of charge variants.
Learn more
Charge heterogeneity assessment with CZE
Suited for:
- Native charge heterogeneity assessment with CZE
- Charge heterogeneity assessment within QC
PA 800 Plus system
Characterize therapeutic molecules with confidence with the kit-based system.
Learn more
All resources
Filter:
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Technical note
Automate DAR calculation at the intact level
Ensure product quality and make ADC analysis accessible with a visually intuitive workflow for routine ADC characterization.
-
Technical note
Enable comprehensive analysis of mAbs and ADCs in a single injection
Gain accurate localization and detailed structural characterization with improved sensitivity for confident detection of fragments.
-
Webinar
Enable mass identification of charge variants and modifications on intact ADCs
Reduce charge heterogeneity analysis from weeks to minutes. Identify and monitor the charged modifications on ADC payloads in near real-time.
-
Webinar
Comprehensive characterization of protein therapeutics
Gain high sequence coverage for proteins and identify critical modifications on a single platform solution.
-
Technical note
Enable faster decision making with high-throughput and intuitive DAR monitoring for ADCs
Streamlined LC-MS based workflows, with user-friendly data analysis software to enable attribute monitoring and informed decision making during process development.
-
Technical note
Eliminate the bottleneck for drug purity analysis with high-throughput rapid CE-SDS purity analysis
Screen a large number of clones for lead clone selection with automation friendly mulit-capillary CE-SDS workflow.
-
Technical note
Accelerate cIEF method development from days to hour
Streamline evalutaion of multiple factors in a single set of experiments with a multifactorial DoE approach.
-
Technical note
Enable a streamlined, accurate and high-throughput glycan analysis assay
High throughput glycan expression profiling with automation friendly sample preparation and labeling.
-
Technical note
Gain in-depth and comprehensive characterization of a new-generation Cys-conjugated ADC
A full characterization workflow of a complex ADC. EAD enabled peptide mapping provides confident identification of T-DXd peptides with partial or complete payload conjugation for accurate DAR determination and impurity assessment.
-
Technical note
Leverage the increased sensitivity of the ZenoTOF 8600 to improve intact protein analysis
Discover how the advantages of increased MS sensitivity for Intact analysis of protein therapeutics, with a workflow to provide better peak separation of glycoforms, and detection of low abundant impurities.
Associated applications
Intact protein analysis
Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.
Learn more
Subunit mass analysis and middle-down
Take control of the unknowns. Gain confident sequence confirmation and localization of post-translational modifications (PTMs) at the sub-unit level.
Learn more
Peptide analysis
Achieve new heights in PTM analysis with confidence and speed. Define critical quality attributes (CQAs) and streamline processes from early to late-stage development with in-depth peptide mapping solutions for next-generation protein therapeutics and standard monoclonal antibodies (mAbs).
Learn more