Overview
Keep with the pace of next generation drug development.
Subunit mass analysis can be used as a screening tool for candidate developability, identity and quality. This approach addresses the need for fast and intuitive characterization of therapeutic proteins in early development to not only ensure drug efficacy and safety, but to quickly distinguish post-translational modifications (PTMs) and drug conjugation sites.
Novel, time-saving strategies for monoclonal antibody (mAb) subunit protein analysis provide in-depth PTM information and leverage high-quality data to develop routine and reproducible workflows from discovery through commercialization.
Workflow
Rapid middle-down protein analysis
Make decisions on candidate developability or product quality sooner.
A challenge in rapid characterization of therapeutics is obtaining high sequence coverage and in-depth information on PTM localization while minimizing sample preparation artifacts like undesired modifications or loss of information on complex therapeutics like antibody drug conjugates.
Subunit mass analysis strategies – such as middle down mAb fragment analysis, can be employed to address these challenges.
A single-injection, EAD-based, middle-down workflow for subunit mass analysis leverages information-rich and reproducible EAD fragmentation with automated data analysis to consistently deliver high sequence coverage of mAb subunits and enable confident sequence and PTM confirmations.
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Save time with confident high sequence coverage of mAb fragments in one injection
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Achieve in-depth analysis with localization of common PTMs like glycosylation and oxidation
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Improve productivity with minimal method development simple software workflows
Solution
- In-depth characterization of mAb subunits
- Rapid PTM localization
ExionLC AE system
ZenoTOF 7600 system
Biologics Explorer software
All resources
Obtain detailed information about glycosylation at the subunit level with straightforward, visual data interpretation and accurate peak assignment.
Minimize sample preparation and provide in-depth information on critical quality attributes for scientists in early devleopment. Learn more in this webinar
The streamlined EAD-based middle-down workflow enables confident sequence confirmation and PTM analysis in a single injection
Learn more about the power of EAD for characterization of large biomolecules
Enable comparative middle-down analysis for the characterization of biosimilars or sequence variants with visually intuitive data analysis tools.
A middle-down strategy for routine subunit mass analysis with high sequence coverage and localization of PTMs, such as glycosylation and oxidation
Application areas
Charge heterogeneity analysis
Pursue the end goal with confidence. Achieve the full picture and accelerate decisions with comprehensive characterization of biopharmaceutical charge variants.
Intact protein analysis
Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.