Accelerate candidate selection by achieving separation, quantitation, and direct identification of biopharmaceutical charge variants and their proteoforms with icIEF-UV/MS via the Intabio ZT system.
The Intabio ZT system couples icIEF separation and UV detection with high resolution mass spectrometry on the ZenoTOF 7600 system. Experience one integrated workflow with a single, accessible dataset containing the required information to make the right decisions, fast.
The process of identifying individual charge variant components and interpreting their structural differences conventionally takes weeks and requires the use of multiple instruments, with multiple manual steps.
With the Intabio ZT system - separation, quantitation and identification of individual charge variants can be achieved in minutes on a single platform.
Separation of charge variants with imaged capillary isoelectric focusing (icIEF) and quantitation with imaged UV detection occurs within the separation channel
To enable electrospray ionization, electrolyte is introduced through the mobilizer channel near the ESI tip, to re-ionize the charge variants. The electric field is re-oriented to initiate the mobilization of the peaks toward the electrospray “ESI” tip. This novel chemical mobilization process ensures that peak resolution is maintained throughout MS detection.
Separated charge variants are introduced to the mass spectrometer by electrospray ionization for peak identification.
Enable a more detailed characterization approach by leveraging the enhanced capabilities of the powerful ZenoTOF 7600 system with EAD fragmentation technology.
High-throughput product quality characterization with rapid multi-attribute monitoring of intact biotherapeutics and identification of charge variants throughout the drug development pipeline.
Intact protein analysis, charge variant analysis, PTM characterization
Charge variants, glycoforms, PQA assessment
CQA and PQA monitoring
Highly confident identification of CQAs with an integrated icIEF-UV/MS solution that identifies low abundant PTMs at the intact protein level.
Gain advantages in data quality and time with an integrated system that offers high-resolution and high-throughput separation of intact mAbs and their charge variants.
A single platform icIEF-UV/MS workflow offers high sensitivity and selectivity to detect low-abundant proteoforms that could impact product quality.