Overview
The analysis of mRNA is complex due to its fragile nature, heterogeneity and length.
Discover workflows that let you break through the analytical boundaries of in vitro transcribed (IVT) products and provide quality results to confidently address the challenges of mRNA, circRNA and saRNA development.
Determine CQAs, integrity, purity and secondary structures with intuitive solutions that provide meaningful answers to keep your process in progress.
Maintaining mRNA integrity is essential for the manufacture of high-quality LNP vaccines. However, predicting LNP potency from legacy, low-resolution techniques remains challenging. In contrast, the SCIEX PA 800 Plus system equipped with the RNA 9000 Purity & Integrity kit enables robust, high-resolution integrity profiling of mRNA, even for larger constructs, removing a long-standing obstacle in mRNA-LNP quality control.
Workflow
mRNA integrity and purity
mRNA product quality is directly linked to integrity and purity.
Enable control of your RNA products. Assess the purity of full-sized mRNA molecules with the highest quality and resolution, and create pathways for analyzing and understanding these fragile molecules more easily and reliably.
Kit-based solutions let you save time so you can focus on your research and help determine the future of medicine.
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Achieve high-resolution mRNA and impurity separation over an extended size range
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Precise kit solutions with pre-assembled cartridges deliver superior accuracy and precision
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Find relevant impurities (even below 1%) with laser-induced fluorescence (LIF) detection
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Cover your compliance needs through compatibility with the Empower Chromatography Data System (CDS)
mRNA integrity and purity
Solution
- High-quality separation
- Accelerated method development and mRNA sample analysis
BioPhase 8800 system
RNA 9000 Purity & Integrity kit
BioPhase BFS capillary cartridge
Featured resources
Take control of impurities and artifacts in your mRNA-lipid nanoparticle (LNP) products. Achieve high-quality, robust assessment of the integrity and size of mRNA molecules encapsulated in LNPs.
Learn from scientist Jérémie Parot (SINTEF) how to break through the boundaries of mRNA analysis.
Discover how to save time by streamlining your CE data management with the Empower CDS.
mRNA integrity and purity
Solution
PA 800 Plus system
RNA 9000 Purity & Integrity kit
BFS capillary cartridge for the PA 800 Plus system
Workflow
CircRNA
The use of next-generation RNA, such as circular RNA (circRNA)—a type of RNA that is not dependent on CQAs such as 3' poly-A tails or 5' capping—is growing.
Pursue improved RNA-based drugs with the ability to confidently separate circRNA from linear precursors and assess a continuous loop of RNA using high-quality data.
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Take control of your circRNA purity with a %CV of <2%
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Rely on superior separation of impurities with highly sensitive detection
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Save time with automated size determination and quantitation of linear RNA precursors
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Cover your compliance needs through compatibility with the Empower CDS
CircRNA
Solution
- High-quality separation
- Accelerated method development and mRNA sample analysis
BioPhase 8800 system
RNA 9000 Purity & Integrity kit
BioPhase BFS capillary cartridge
CircRNA
Solution
PA 800 Plus system
RNA 9000 Purity & Integrity kit
BFS capillary cartridge for the PA 800 Plus system
Workflow
5’ capping
Imagine taking control of your mRNA stability and translation efficiency by better understanding your 5’ CQAs.
Characterize and quantify capped vs. uncapped mRNA species and dig deeper into distinguishing different capping intermediates with high-quality data generated using solutions based on capillary electrophoresis (CE) and accurate mass spectrometry.
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Clearly identify capping intermediates (G cap, cap 0 and cap 1) with accurate mass information
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Break free from quantitation limitations with accurate mass quantitation
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Differentiate capped from uncapped 5’ ends with high-quality CE solutions
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Be prepared for a method transfer to quality control (QC) with reliable CE solutions
5’ capping
Solution
- Identification and quantitation of 5'-end capping intermediates
- Robust, analytical flow setup
ExionLC AE system
X500B QTOF system
Molecule Profiler software
5’ capping
Solution
- Identification and highly sensitive quantitation of capping intermediates
- Meeting flexibility needs to perform a range of additional workflows
ExionLC AE system
ZenoTOF 7600 system
Molecule Profiler software
5’ capping
Solution
- Differentiation of capped from non-capped 5' ends
- Assay transfer to quality control (QC)
PA 800 Plus system
ssDNA 100-R kit
Workflow
3’ poly-A tail
Understanding product stability lays the foundation for improved drugs.
Lead the way by characterizing your 3’-end CQAs, whether you are using template-encoded tails, enzymatic addition or a combination of these approaches.
Give your team the power to determine the lengths of the adenosine tails and their distribution profiles in mature mRNA products with solutions based on CE and accurate mass systems.
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Progress your mRNA development with a clear understanding of your poly-A tails
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Access poly-A tail length information via deconvolution of high-quality accurate mass data
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Obtain size information for 3’ tails with excellent base resolution via CE
3’ poly-A tail
Solution
- Molecular weight determination of 3'-end poly-A tails
- Robust, analytical flow setup
ExionLC AE system
X500B QTOF system
SCIEX OS software
3’ poly-A tail
Solution
- Molecular weight determination of 3'-end poly-A tails
- Meeting flexibility needs to perform a range of additional workflows
ExionLC AE system
ZenoTOF 7600 system
SCIEX OS software
3’ poly-A tail
Solution
PA 800 Plus system
ssDNA 100-R kit
Workflow
Secondary structure
RNA structures can affect your LNP formulations and the stability and efficacy of a final product. Gain greater control of structural prediction for your oligonucleotides of interest. Leverage solutions that gather empirical data without nucleotide or structural bias to enable better insights into RNA backbone flexibility based on base-paired and unconstrained residues.
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Achieve structural understanding with data-driven information
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Find hotspots for backbone flexibility in your nucleic acid products
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Benefit from data without nucleotide bias
Secondary structure
Solution
GenomeLab GeXP system
All resources
Break through the barriers to developing better LNP-based drugs and make better use of your time with streamlined CE and MS workflows for mRNA-LNPs.
Self-amplifying RNA (saRNA) can enable lower dosing and expand the range of potential indications. However, srRNAs are often much larger in size (>9,000 bases). Learn how to assess these large molecules with high-resolution assays.
Take control of impurities and artifacts in your mRNA-lipid nanoparticle (LNP) products. Achieve high-quality, robust assessment of the integrity and size of mRNA molecules encapsulated in LNPs.
Learn from scientist Jérémie Parot (SINTEF) how to break through the boundaries of mRNA analysis.
Discover how to save time by streamlining your CE data management with the Empower CDS.
Set your own schedule for mRNA analyses by streamlining your CE data management with the Empower CDS.
Create pathways for effective method development and learn how to achieve the highest quality data for RNA products with sizes ranging from 50–9,000 nucleotides and beyond.
Pave the way to better characterization of circRNA generated from linear precursors and take your RNA analysis to the next level.
Associated applications
CRISPR/Cas9 analysis
Set your own schedule for CRISPR/Cas9 gene editing with innovative, intuitive analytical solutions. Unleash the potential of your Cas9 messenger RNA (mRNA), single guide RNA (sgRNA) and Cas9 proteins by understanding quality, purity and safety and assessing on/off target effects.
Lipid nanoparticles and non-viral carriers
Expand the possibilities of drug delivery with innovative analytical solutions for lipid raw materials, LNPs and other non-viral carriers. Move beyond boundaries in structural elucidation and monitoring of analytes to estabilish confidence in the quality and stablity of your products.
Plasmid and DNA analysis
Advance your projects with purpose-built solutions for the analysis of plasmid DNA (pDNA) and double-stranded DNA (dsDNA) to support the development of your messenger RNA (mRNA), viral vectors, recombinant proteins and other new modalities.