Amplifex™ Keto Reagent

Signal enhancement maximized through intelligent chemistry

For research use only. Not for use in diagnostic procedures.

Amplifex Keto Reagent is designed specifically to enhance signal strength up to 500-fold (depending on the analyte). 

The Amplifex Keto Reagent is a universal reagent that reacts with a molecule's keto or aldehyde functional group, such as the ketone in ketosteroids (e.g., testosterone). 

With the Amplifex Keto Reagent,  your sample size can be dramatically reduced, while still achieving LLOD and LLOQ levels that were difficult, if not impossible, by mass spectrometry analysis even on the most-sensitive platforms.

  • Intelligent design boosts ionization efficiency and improves fragmentation
  • Substantial reduction in sample volume and analysis time is enabled.
  • Reactions are simple (one-step derivatization) and fast (30-60 minutes).
  • Universal derivatization technique reacts with all molecules containing the keto or aldehyde functional groups

Status: Available Item status information

Overview

Designed specifically to enhance sensitivity of trace analytes and enable workflows that push the limits of sample volume

Amplifex Keto is a customized derivatizing reagent designed to react specifically with a molecule's keto and aldehyde functional groups  to improve ionization efficiency.   (i.e. Amplifex™ Keto Reagent treatment results in a sensitivity enhancement of ~40-fold in testosterone and universally reacts with all ketosteroids across an array of sources (plasma, dried blood spot, dialyzed samples, urine, and oral fluids).

Signal enhancement for testosterone

Amplifex Keto Reagent is designed to boost signal intensity for all ketosteroids. Here is an example of a 40-fold increase in sensitivity for testosterone.

Signal enhancement for molecules with keto or aldehyde funct

Amplifex Keto Reagent is intelligently designed to derivatize any molecule's keto or aldehyde functional groups. See examples of additional compounds.

Derivatizes across an array of matrices

Amplifex Keto Reagent is not limited to one sample matrix. The reagent will derivatize molecules in dried blood spots, plasma, dialyzed samples, urine, and oral fluids.

Derivatizes across an array of matrices

Amplifex Keto Reagent is not limited to one sample matrix. The reagent will derivatize molecules with dried blood spots, plasma, dialyzed samples, urine, and oral fluids.

Amplifex Keto Reagent workflow

The Amplifex Keto Reagent derivatization and sample preparation workflows are quick (30 minutes), simple (one-step), reproducible, robust, and high-yielding regardless of sample matrix.

 

Kits

 

Part Number

Kit Name/ Description

4465962

Amplifex Keto Reagent Kit
Contains a protocol, 2 tubes of Amplifex Keto reagent, and 2 tubes of diluent. Each tube contains enough reagent to complete 25 reactions

4466055

Amplifex Keto Prep Starter Kit
Contains hardware components required for Amplifex Keto sample prep. Includes 24-pack C-200 cartridges, 24 unit rack, and protocol.

4466054

Amplifex Keto Cartridge Kit
Contains cartridges for Amplifex Keto sample prep. Includes 24-pack C-200 cartridges and sample prep protocol.

4466179

Amplifex Keto Plate Kit
Contains components required for increased sample throughput. Includes one 96-well plate with C-200 media, one collection plate, and protocol.

5028174

Amplifex Prep Replacement Solvent Guide
12-pack of replacement solvent guides.

 

Resources

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