Project Goal

In colorectal and breast cancers, we use proteomics to identify a senescence secretome to find biomarkers that could predict the efficacy of treatments.

The Solution

• The TripleTOF® 5600+ System, a robust LC-MS/MS platform working at high throughput in microflow mode. It’s way faster – 1 hour per sample - and more robust compared to nano flow with similar sensitivity.
• We use SWATH Acquisition to get comprehensive coverage and high quality quantitation across many samples.

Biggest Challenges Right Now

• Ability to identify and quantify as many proteins as possible in our samples
• Acquisition of reproducible quantitative data
• Creation of permanent digital record of the proteome for each sample

The Outcomes

• We can work on very large cohorts (more than 150 tumor samples) to identify robust biomarkers or to better understand the cancer biology.