Pesticide screening typically requires large numbers of compounds to be screened in a single method and these methods are routinely used to in food testing labs. In order to successfully detect and quantify large numbers of pesticides, time-scheduled methods with fast run times are required. Having quality separations with highly reproducible retention times is critical for success, to minimize time spent adjusting methods and reduce data loss. Here the reproducibility of the ExionLC 2.0 system for the LC-MS analysis of a large panel of 200 pesticides is assessed.