iMethod™ Application for NIDA 5 Drugs Version 1.1 for Cliquid® Software

For research use only. Not for use in diagnostic procedures.

The most commonly-used screening category for drugs of abuse is called the NIDA-5 (National Institute on Drug Abuse), which include cannabinoids, opiates, amphetamines, cocaine, and phencyclidine (PCP). In urine, the target drugs and/or metabolites are amphetamine, methamphetamine, PCP, codeine, morphine, benzoylecgonine, 6-monoacetylmorphine (6-MAM), and (±)-11-nor-9-carboxy-Δ9-THC (THC-COOH). Laboratory-based drug testing is usually performed in a two-step fashion using two different detection methods. The first step is a screening test and is usually done by immunoassay (ELISA, EMIT, or RIA). Suspected positive samples are confirmed using mass spectrometry (commonly GC/MS). Recently, LC/MS/MS has emerged as a reliable method to perform both drug screening and confirmation in urine without the setup requirements of immunoassays or the time-consuming sample preparation and derivatization required for GC/MS confirmation. Under SAMHSA (Substance Abuse and Mental Health Services Administration), NIDA's current guidelines for federally-mandated testing, urine is the only specimen included for testing government employees and private sector employees covered under the US Department of Transportation or other agency guidelines. The described sample extraction technique provides an effective means to detect and quantify drugs of abuse in urine at or below the typical GC/MS cutoff values.

To learn more, please view this flyer: A Rapid iMethod™ Application for the Analysis of Drugs of Abuse in Urine

iMethod Application for NIDA 5 Drugs Version 1.1 for Cliquid Software


This method includes all documentation, MS and LC method files, processing files, and report templates to deploy this new iMethod™ Application in Cliquid® Software. It is suggested that all documentation be reviewed completely prior to using the enclosed test.

Reduce development time


Activity Using an iMethod™ Application Traditional Method Development
Complete Test Protocol Documentation Included Weeks–Months
Sample Prep Techniques Included, with examples and references Weeks–Months
Optimized MS/MS Acquisition Parameters Included Weeks–Months
LC Instrument Parameters with Retention Times Included Days–Weeks
Integration (Quantitation) Parameters Included Days
Reporting Templates Included Days
LC/MS/MS Library Spectra May be included. Stand-alone libraries are also available. Weeks–Months
Staff Training – Software Simple Cliquid® Software interface, no additional training Traditional MS Software and review procedures – Days
Method Validation Weeks. Validation templates and support available Weeks–Months
Complete Test Development and Deployment Weeks 6–12 months