Is it necessary to have .wiff, .wiff.scan and wiff2 files together in one folder in order to open data in SCIEX OS software ?

For research use only. Not for use in diagnostic procedures.

Answer

SCIEX OS generates three types of wiff files: .wiff2 / .wiff / .wiff.scan. The software stores TIC and method data in .wiff2 and .wiff files. The .wiff.scan file is used to store spectral data. SCIEX OS generates both a .wiff2 and .wiff file so that the acquired data can also be reprocessed in Analyst® and MultiQuant, as these two softwares are unable to use .wiff2 files.

The lists below indicates the impact of deleting the .wiff/.wiff.scan files when using the SCIEX OS software.

List of features available in SCIEX OS if the .wiff file is deleted and only the .wiff2 and .wiff.scan files are used:

• Data can be opened in the explore section of SCIEX OS.
• A new results table can be created in SCIEX OS from raw data.
• A previously saved results table can be opened and it's possible to view the chromatograms from those results tables in SCIEX OS.

List of features available in SCIEX OS if the .wiff and .wiff.scan files are deleted and only the .wiff2 file is used:

• Data can be opened in the explore section of SCIEX OS.
• A new results table cannot be created in SCIEX OS from raw data.
• A previously saved results table can be opened, but it's not possible to view the chromatograms from those results tables in SCIEX OS.