Course Detail

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3 day advanced LC-MS/MS quantitation: method development and troubleshooting at SCIEX


  • Overview: This SCIEX Now Learning Hub course is designed to provide experienced users with the knowledge necessary to successfully perform LC-MS method dvelopment with a focus on small molecule quantitation and troubleshoot common MS and HPLC issues. Upon completion of the course, you will be comfortable with performing instrument tuning and calibrating, optimizing a compound in ESI and APCI mode, understanding how to build a robust LC-MS method, troubleshooting common issues with method development and your LC-MS system, understanding quantitation parameters, and identifying issues with your processed results. This course provides a variety of hands-on exercises, best practices and finished with a troubleshooting challenge. It is delivered at a SCIEX training facility by an experienced SCIEX instructor. 
  • Who should attend: Learners who need to gain knowledge necessary to successfully perform LC-MS method development and to troubleshoot and fix common MS and HPLC issues.
  • Pre-requisites: This course is intended for those who have completed a Success Program or have at least one year of operational experience with SCIEX LC-MS systems.
  • What’s included:
    • 3 days of instructor led training at a SCIEX training facility by an experienced instructor.
    • Tuition for 1 learner to participate (travel and living expenses NOT included).  
    • Online workflow certificate upon successful completion of final exam.
    • P.A.C.E.® Continuing Education Credits: 18 credits upon completion of entire training session and a brief evaluation survey. 
    • Enrollment in related self-paced eLearning courses, lectures, reference material and lab exercises on the Learning Hub.
    • Permanent access to all course materials for reference.
    • Access to > 100 SCIEX Now Learning Hub self-paced eLearning, lectures, demonstrations as well as SCIEX Now online support tools.  
    • Valid for 12 months from date of purchase.
  • Related instruments: This course is currently offered for the following instruments – 
    • LC systems: Demonstrations will be performed on Shimadzu systems.  Content will be provided for SCIEX ExionLC and Jasper LC systems as well as Shimadzu (LC-20AD, LC-20ADXR, LC-20AT, LC-30AD) and Agilent (1260, 1260 Infinity II, 1290, 1290 Infinity II).
    • MS instruments: ALL SCIEX Triple Quad and QTRAP systems (RUO and MD versions).  Please note that your exact model may not be available at all locations but the procedures will be same or similar and will include content relevant for all.
  • Related workflows: Quantitation of small molecules in clinical diagnostics, clinical research, forensics, food or environmental testing and pharma/CRO.   
  • Training location: SCIEX training facility
  • Follow on courses:
    • TRNSU201                    2.5 day advanced LC-MS maintenance and troubleshooting at SCIEX
    • TRNSU212                    2 day LC-MS method validation at SCIEX
    • TRNVT212                    4 day LC-MS method validation virtual

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