Project Goal

To analyze cohorts of IgGs and mAb based modalities using mass spectrometry characterization using a complete analytical workflow on a single workhorse instrument.

The Challenges

• Require a single, complete MS platform that can handle a diversity of the analytical workflows- intact mass, peptide mapping in reduced and non-reduced conditions, and relative quantitation of post-translational modifications
• Need an easy-to-use MS system that does not require extensive training. Walk-up resource, ready to use instantly
• Want automated calculations and customized reporting for data that relies on minimal external intervention

The Solution

The X500B QTOF System, a robust LC-MS platform with:

• Good sensitivity & linear dynamic range
• User-friendly data acquisition system
• Dedicated BioPharmaView™ Software to do a full characterization of biotherapeutics

The Outcomes

• After a quick calibration, the X500B QTOF is ready to use within minutes
• The system is easy-to-use. New users in the team can be trained within a few hours, and are completely operational
• After six-months of operation, and around 2000 injections, no downtime has occurred, and there has been minimal cleaning of the source
• The BioPharmaView software solution allows for step-by-step biotherapeutic characterization, for naked antibodies and even immunoconjugates