Project Goal

In a novel model system of metastatic prostate cancer, we use proteomics and genomics profiling to understand the biological mechanisms of metastasis and find biomarkers of disease state and therapeutic response.

The Challenges

• Creation of permanent, quantitative, digital record of the proteome
• Processing large amounts of SWATH® Acquisition data and generating biological conclusions
• Compare proteomics data with transcriptomics

The Solution

• Employing state-of-the-art Omics technologies that provide comprehensive quantitative profiles

The Outcomes

• Using SWATH Acquisition, we can quickly create proteomic profiles of a large # of proteins in each sample (up to 4,500 in an hour’s LC gradient), which enables us to run many different samples to characterize the biology, like cancer vs. non-cancer, or aggressive vs. non-aggressive, for example.
• Achieving 20-50 samples MORE per week than previous methods.
• The better we understand the mechanisms of metastatic cancer, the better we can develop new therapies and eventually tailor these to the right type of cancer