Seminar details:

Sunday, August 14, 2022 | 11:50 a.m.-1:30 p.m.
Auditorium | Broad Institute of MIT and Harvard

SCIEX Sunday lunch seminar

Register today for this can’t-miss seminar at ASBMB’s 14th International Symposium on Mass Spectrometry in the Health and Life Sciences..

Lunch will be provided for those who pre-register. 

Presentations

High-throughput Zeno SWATH DIA analysis of ischemic acute kidney injury using a fast-scanning ZenoTOF 7600 system

Birgit Schilling, PhD, Director of the Mass Spectrometry Core and an Associate Professor at The Buck Institute for Research on Aging

The development of fast scanning mass spectrometers, such as the ZenoTOF 7600, with a highly efficient duty cycle to transfer analyte ions to the TOF detector has enabled to efficiently analyze biologically relevant samples. In addition, the improvements in sensitivity and selectivity using the Zeno Trap, as well as the speed of analysis by using microflow chromatography has been highly beneficial. We will apply this fast ZenoTOF scanning, Zeno Trap technology and a novel Zeno SWATH DIA workflow to a kidney injury model that is highly relevant for human health. The occurrence of acute kidney injury (AKI), and subsequent decrease in kidney function, increased 230% from 2000 to 2014, and represents a significant burden on health-care systems worldwide. In this study, kidneys from wild-type mice were subjected to ischemia-reperfusion injury while the contralateral kidneys remained uninjured to assess the changes in the kidney proteome. Upon AKI the kidney proteome was completely remodeled, and over half of the 3,945 quantified protein groups (>=2 confident peptides) were significantly changed. The comprehensive and sensitive kidney-specific Zeno SWATH DIA assay, presented in this work, features high-throughput, fast scanning, deep coverage of the kidney proteome, and will serve as useful tool for the development of novel therapeutics aimed at remediating kidney function.

Maximizing the TOF duty cycle enhancement via Zeno trap to significantly improve the Proteomes

Jose Castro-Perez, Senior Director, Market Development, SCIEX

Rapid and robust proteomes with standardized methods on the Evosep One powered by Zeno SWATH DIA

Dr. Nicolai Bache, Head of Applications, Evosep

Mass spectrometry-based clinical proteomics is becoming increasingly popular and provides a powerful set of technologies, with the potential to revolutionize health care and enable precision medicine. We specifically designed and developed the Evosep One for high-throughput applications, with a focus on clinical analysis of large sample cohorts. The newest and most powerful generation of mass spectrometers combined with novel acquisition strategies and software tools keep pushing boundaries and are now making comprehensive proteomes in short time a reality. We evaluated the new ZenoTOF 7600 mass spectrometer system with the novel Zeno SWATH DIA acquisition strategy across the Evosep One standard methods. For high-throughput applications with 200 samples analyzed per day, we were able to reproducibly identify and quantify more than 4,000 proteins from 200 ng HeLa digest. Collectively, our results show that Zeno SWATH DIA is a significant driver of deep proteomes, making the ZenoTOF 7600 system an ideal match with high-throughput and standardized methods on the Evosep One.

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