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5-(4’-(azidomethyl)-[1,1’-biphenyl]-2yl)-1H-tetrazole (AZBT) is a known impurity found in sartan medications and there is some concern that AZBT could act as a mutagen. Here, an assay has been developed for the sensitive detection of the AZBT impurity in an irbesartan drug substance and a candesartan drug product using the QTRAP 4500 system. Excellent sensitivity was achieved with lower limits of quantification of 0.5 ng/mL which is well below the threshold of toxicological concern (TTC) for these drugs. With a total run time of 8 minutes, this robust method provides require sensitivity, linearity and recovery for assessing levels in APIs and drug products.

In recent years, oral fluid has gained considerable attention as a quicker and less invasive means of monitoring cannabis use. More specifically, the use of this matrix for drug testing benefits from ease of sampling, observed collection and difficulty of sample adulteration. Here we demonstrate the key advantages of the SCIEX Triple Quad 7500 System – QTRAP Ready for sensitive detection of THC-COOH in oral fluid.

China is the world’s home of tea and is the first country in the world to discover, cultivate, make and drink tea. However, driven by profit, the tea crisis is becoming increasingly important. This is mainly highlighted by the “poisoning” of tea quality. This application note focuses on the problem of pesticide residues in tea. On the SCIEX Triple Quad 3500 System, a rapid screening method of 222 pesticides has been established to provide a simple and quick solution to the problem of pesticide residues in tea.

A targeted LC-MS/MS assay with MRM transitions for over 450 metabolites was used to assess the impact of added sensitivity on the detection and quantification of metabolites in plasma. Using a generic reversed-phase chromatography method and operating the MS systems in both positive and negative polarity modes to obtain broad coverage, a similar method was run on three separate QTRAP 6500+ systems and as well as three separate SCIEX 7500 systems under independent lab settings.

In recent years, animal mycotoxin poisoning incidents have occurred frequently in China and around the world, causing huge economic losses to the agriculture industry. This work focuses on the detection of mycotoxins in animal feed. On the SCIEX Triple Quad 3500 System, a rapid determination method of 30 mycotoxins was established. This method provides a simple and quick solution for the detection of mycotoxins in the feed.

A method for rapid quantification of TMAO, betaine, L-carnitine, acetyl-carnitine, and choline in plasma has been developed on the QTRAP 4500MD system. This method has the advantages of high specificity, linearity, and high accuracy. As shown, this method is suitable to support rapid monitoring of TMAO metabolites: • Rapid 5.5-minute run time provided good peak separation for detection in a shorter run time than several recently published methods for faster screening. • Consistent quantification results were obtained for all standards tested, with correlation coefficients ranging from 0.991 to 0.999 • Inter- and intra-day reproducibility and accuracy meet standard bioanalytical requirements of %CV less than ≤ 15% in for all analytes analyzed in plasma • Method was evaluated in plasma matrix using a simple extraction protocol and good signal/noise was observed.

Using the SCIEX QTRAP 4500 System, EPA Method 537.1 was used for the analysis of a suite of 14 per- and polyfluorinated substances (PFAS) in drinking water.

To ensure public health and safety, many government agencies establish routine monitoring systems for pesticide residues in agricultural products such as vegetables and fruits. Many of these residues are amenable to LC-MS/MS analysis. The method developed here can be used to simultaneously analyze 51 pesticide residues that are routinely monitored by many health agencies.

The SCIEX Triple Quad 4500 system coupled to an ExionLC AC system can achieve, and in many cases surpass, target MRLs for PFAS residues specified in EPA method 533 and UCMR5. Simple upgrades to the LC system can limit contamination and separate background contaminants from residues in the analytical injection. The method was able to successfully test for PFAS contamination in New England water samples• Simple upgrades to the LC system can limit contamination and separate background contaminants from residues in the analytical injection. The method was able to successfully test for PFAS contamination in New England water samples

This technical note demonstrates the trace level quantification of anatoxin-a and cylindrospermopsin, following EPA Method 545, to achieve detection limits of 0.005 ng/mL. The detection limits were 60x and 140x below the microcystin and cylindrospermopsin health advisory levels (HALs) for young children. The sensitivity of the SCIEX 7500 system allowed for a simple, direct injection of 5 µL. In addition, an LC run time of 10 minutes minimized co-eluting interferences while maintaining throughput. Matrix applicability was shown by spiking four individual water samples at two levels, and all water samples showed excellent accuracy (<±20%) and precision (<10%).