An important part of the biopharma industry, plasmid DNA is used as a gene-delivery vehicle for DNA vaccination and as a key intermediate for processes like viral particle production for gene therapy and ex-vivo protein synthesis. Most plasmid DNA preparations contain several topological variants or isoforms including the supercoiled, open-circular and linear forms of the molecule.
Federal regulations require purity testing for manufactured injectable plasmid products and recommend establishing a release criterion of >80% supercoiled content. Unfortunately, traditional agarose gel has limitations on quantification. In this webinar, we present a method for plasmid analysis by CE-LIF with the use of a common dye. This method is sensitive and robust, as well as suitable for testing plasmid purity and monitoring plasmid degradation.
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Related to -RUO-MKT-11-10859-A