A sensitive and robust plasmid analysis method by Capillary Electrophoresis with Laser-Induced Fluorescence detection

A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

An important part of the biopharma industry, plasmid DNA is used as a gene-delivery vehicle for DNA vaccination and as a key intermediate for processes like viral particle production for gene therapy and ex-vivo protein synthesis. Most plasmid DNA preparations contain several topological variants or isoforms including the supercoiled, open-circular and linear forms of the molecule.

Federal regulations require purity testing for manufactured injectable plasmid products and recommend establishing a release criterion of >80% supercoiled content. Unfortunately, traditional agarose gel has limitations on quantification. In this webinar, we present a method for plasmid analysis by CE-LIF with the use of a common dye. This method is sensitive and robust, as well as suitable for testing plasmid purity and monitoring plasmid degradation.

You will learn about:

  • Fast analysis of plasmid samples with separation time within 12 minutes
  • Baseline resolution of supercoiled, linear and open circular isoforms of a 5 kb and a 7 kb plasmid
  • Streamlining workflows using commercially available dsDNA 1000 kit and LIFluor EnhanCE stain
  • How automated sample separation and data analysis provide rapid, reproducible and quantitative results with RSD of 0.23% for migration time and 0.61% for % area

Who should attend:

  • Lab scientists or managers developing gene therapies
  • Those interested in analytical solutions for plasmid DNA

Speaker

Related to -RUO-MKT-11-10859-A

Watch On-Demand: