Inside the technote: Parkinson disease

Low-pg/mL quantitation of leucine-rich repeat kinase 2 (LRRK2) protein in human cerebrospinal fluid (CSF)

The exact cause of Parkinson disease remains unknown, but research has shown that mutations in the gene encoding LRRK2 is one of the most common causes of the disease. Jack Steed, Senior Technical Specialist at SCIEX, looks at this technical note which demonstrates a very robust and sensitive method for the quantitation of the protein LRRK2 in human CSF using the SCIEX 7500 triple quadrupole mass spectrometry coupled to the M5 MicroLC system.

Key learnings:

• Low pg/mL level of quantitation: Achieve 10 pg/mL LLOQ for quantitation of LRRK2 protein in human CSF
• Enhanced sensitivity: Achieve a 2.5-fold improvement in LLOQ and a 5-fold improvement in signal-to-noise (S/N) using the M5 MicroLC system compared to conventional analytical flow conditions
• Lower solvent consumption: The trap-and-elute workflow uses up to 50x less solvent than the analytical flow method, resulting in significant cost savings, waste reduction and a more sustainable solution
• Robust analytical performance: Achieve accurate quantitative performance with %CV <10% at all concentration levels across a linear dynamic range (LDR) of 3 orders of magnitude

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