- Productos de ciencias biológicas e investigación para espectrometría de masas
- iMethod Applications
The Power of Precision
The SCIEX iMethod Application for the aTRAQ Kit 1.0 with Cliquid 3.2.1 Software for routine amino acid analysis provides an easy-to-learn, easy-to-use, browser-based application. Through licensing, the Cliquid AA functionality is accessible through the Cliquid 3.0 Software that automates the process of acquiring data and generating reports.
The iMethod Application for aTRAQ Kit benefits from the sensitivity and specificity of LC-MS/MS analysis using either a Triple Quad or QTRAP System operating in MRM mode. HPLC separation is performed using a C18 column. The gradient, wash, and equilibration require a total of 18 minutes, maximizing throughput. The use of the Scheduled MRM™ Algorithm maximizes dwell time while monitoring of large numbers of MRM transitions, resulting in optimum data quality and reproducibility.
This iMethod Application also employs aTRAQ Reagents to increase the precision and accuracy of quantitation by providing an internal standard for each amino acid analyzed. The aTRAQ Reagents also provide an extra degree of validation, using the retention time and the molecular weight of the standard for confirmation of each amino acid's identity.
To learn more, please view this flyer: A Rapid iMethod™ Application for the Analysis of Amino Acids
Reduce development time
|Activity||Using an iMethod Application||Traditional Method Development|
|Complete Test Protocol Documentation||Included||Weeks–Months|
|Sample Prep Techniques||Included, with examples and references||Weeks–Months|
|Optimized MS/MS Acquisition Parameters||Included||Weeks–Months|
|LC Instrument Parameters with Retention Times||Included||Days–Weeks|
|Integration (Quantitation) Parameters||Included||Days|
|LC/MS/MS Library Spectra||May be included. Stand-alone libraries are also available.||Weeks–Months|
|Staff Training – Software||Simple Cliquid® Software interface, no additional training||Traditional MS Software and review procedures – Days|
|Method Validation||Weeks. Validation templates and support available||Weeks–Months|
|Complete Test Development and Deployment||Weeks||6–12 months|