iMethod™ Application for THC-COOH for Cliquid® Software (Partner Contributed)
For research use only. Not for use in diagnostic procedures.
Cannabis use is detected by identifying the presence of the major psychoactive constituent of marijuana, Δ9-tetrahydrocannabinol (THC), and its metabolites in biological fluids (e.g. urine). The major metabolite found in urine is 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH), which exists in both the free and glucuronide form.
Samples are prepared by the dilution and hydrolysis of urine to remove any glucuronidates followed by automated, online clean-up using a Spark Holland HySphere C8EC-SE sorbent cartridge upon injection in a Symbiosis PICO system. More in-depth sample preparation and instrument parameter information is included with the method, as well as the required analytical columns. Solvents, standards, and any supplies required for sample preparation are not included.