iMethod™ Application for THC-COOH for Cliquid® Software (Partner Contributed)

For research use only. Not for use in diagnostic procedures.

Cannabis use is detected by identifying the presence of the major psychoactive constituent of marijuana, Δ9-tetrahydrocannabinol (THC),  and its metabolites in biological fluids (e.g. urine). The major metabolite found in urine is 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH), which exists in both the free and glucuronide form.

iMethod Application for THC-COOH for Cliquid Software Partner Contributed


Samples are prepared by the dilution and hydrolysis of urine to remove any glucuronidates followed by automated, online clean-up using a Spark Holland HySphere C8EC-SE sorbent cartridge upon injection in a Symbiosis PICO system. More in-depth sample preparation and instrument parameter information is included with the method, as well as the required analytical columns. Solvents, standards, and any supplies required for sample preparation are not included.

For system requirements and example results, read the flyer: iMethod™ Application for THC-COOH for Cliquid® Software.

Reduce development time

ActivityUsing an iMethod ApplicationTraditional Method Development
Complete Test Protocol DocumentationIncludedWeeks–Months
Sample Prep TechniquesIncluded, with examples and referencesWeeks–Months
Optimized MS/MS Acquisition ParametersIncludedWeeks–Months
LC Instrument Parameters with Retention TimesIncludedDays–Weeks
Integration (Quantitation) ParametersIncludedDays
Reporting TemplatesIncludedDays
LC/MS/MS Library SpectraMay be included. Stand-alone libraries are also available.Weeks–Months
Staff Training – SoftwareSimple Cliquid® Software interface, no additional trainingTraditional MS Software and review procedures – Days
Method ValidationWeeks. Validation templates and support availableWeeks–Months
Complete Test Development and DeploymentWeeks6–12 months