Choosing the Peak Selection Criteria when Using SCIEX OS AutoPeak Integration Algorithm


Date: 02/15/2019
Categories: SCIEX OS

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For research use only. Not for use in diagnostic procedures.


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There are four types of peak selection criteria that can be applied to a SCIEX OS quantitation method: Expected RT, IS, Largest, and Group. These can be selected when working with the AutoPeak integration algorithm under Project Default Settings under the Projects menu in the Analytics mode.

  • Use Expected RT when the retention time (RT) is known and you are not using stable-labeled isotopes for your internal standard (IS). Only one fragment per analyte is allowed.
  • Use Largest when the RT is not known and you are not using stable-labeled isotopes for the IS. Only one fragment per analyte is allowed.
  • Use IS when using a stable-labeled IS for each analyte.
  • Use Group to monitor two or more fragment ions from the same precursor ion.
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