For TOFMS, all ions pass through Q1 in RF mode. In general, Q1 and Q2 perform "hops" over the mass range, ensuring an appropriate envelope of masses is passed into the TOF analyzer. The general explanation found here for the QSTAR® systems can also be used to explain TripleTOF® systems as well.
Chemoview™ software error after data processing "Programmatic access to Visual Basic project is not trusted" can be resolved by turning on the access to the VBA object model from any automation client.
The collision energy (CE) and the collision energy spread (CES) needed for peptide analysis (during protein IDA identification and SWATH™ acquisition) can be optimized using the rolling CE setting embedded in the Analyst® TF software.
While running Analyst® software in Manual Tune mode during compound optimization, an error message is received: "The current sample cannot be run because the syringe pump has stalled. The syringe plunger may be stalled in the syringe barrel." To resolve this issue, move the syringe holder back and clear error. If this doesn't work, replace the syringe, or if still needed, reset the MS.