For research use only. Not for use in diagnostic procedures.
Answer
The Intact Quant feature in Sciex OS 1.7 software platform enables the quantitative processing of intact proteins without sample prep and allows for the complete and compliant intact reconstruction of protein data sets. To activate this feature, an additional, add-on software license, SCIEX OS IntactQuant 1.7 (part number 5078597), must be installed.
To enable the IntactQuant feature in SCIEX OS software, follow these steps:
- Activate the IntactQuant license and copy the license file to this location: C:\ Program Files\SCIEX\SCIEX OS.
- Launch the Sciex OS 1.7 software.
- Open the Analytics module.
- Select the Project menu and then select the Default Settings (see image).
- Highlight the Mass Reconstruction Processing tab. Select the MQ4 integration algorithm from Integration Algorithm drop down list.
- Adjust any other reconstruction processing method parameters here and save.
To enable the IntactQuant feature in a method, open a new processing method, select the Quantitation workflow and load a sample. On the Components tab, under the Options menu, select the Mass Reconstruction option and a popup screen will appear asking if the mass reconstruction option should be turned on. Select the Yes button. A check mark will now appear next to the Mass Reconstruction menu item.
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