Abstract
This technical note describes a highly sensitive quantification workflow for peptides using an in-sample calibration approach on a high-end triple quadrupole mass spectrometer. Peptide quantification with lower limit of quantification (LLOQ) as low as 1 amol was achieved (Figure 1) with excellent accuracy, linearity and reproducibility.
Introduction
Given their high target specificity, protein and peptide therapeutics are becoming increasingly important therapeutic products. As a result, there is a strong demand for the generation of highly accurate and sensitive bioanalytical methods to ensure proper quantitative measurement throughout the biopharmaceutical development process. Current methods for the bioanalysis of peptide therapeutics are often met with challenges involving limited sensitivity and the presence of matrix interferences.
Herein, a SCIEX 7500 system was used for the quantification of a series of peptides in matrix. Multiple hardware improvements on the ion source and the front-end of the mass analyzer significantly boosted the systems sensitivity and %CV for low-level quantification.1,2 This technical note summarizes an in-sample calibration approach applied to evaluate specificity and sensitivity for quantifying peptides using a LC-MS/MS platform.
Key features of the peptide quantification workflow on the SCIEX 7500 system
- Achieve as low as 1 amol LLOQ for peptide quantification using a highly sensitive triple quadrupole mass spectrometer
- Reach outstanding sensitivity for peptide quantification with improved front-end technology enabling greater ion generation and transmission
- Ensure superior quantitative performance with excellent reproducibility, accuracy and linearity
- Increase productivity with the user-friendly interface and integrated platform for data acquisition, processing and management in the SCIEX OS software
Methods
Samples and reagents: The 6x5 LC-MS/MS peptide reference mix was purchased from Promega.
Sample preparation: The reference set contains 6 peptides with a mixture of 5 isotopologues for each peptide. The isotopologues are introduced into the mixture with a 10-fold molar increase. Therefore, with each sample injection, a linear range of 4 orders of magnitude was examined for each of the 6 peptides.
Samples were prepared by spiking the 6x5 peptide mix into a trypsin digest of BSA. The BSA digest was prepared at a concentration of 60 fmol/µL in 0.1% formic acid with 2% acetonitrile in water. Two samples were prepared, with the final concentration of the peptides ranging from 1 amol/µL to 10 fmol/µL and 10 amol/µL to 100 fmol//µL, each in 60 fmol/µL BSA digest.
Chromatography: An ExionLC system was used for analyte separation. Gradient and other chromatographic conditions for the assay are summarized in Tables 1 and 2. A volume of 1 µL or 5 µL of the sample was loaded for analysis. The flow rate was set to 0.6 mL/min.
In-sample calibration workflow
The peptide reference matrix contained the 6 peptides VTSGSTSTSR, LASVSVSR, YVYVADVAAK, VVGGLVALR, LLSLGAGEFK and LGFTDLFSK. For each peptide, a mixture of 5 isotopologues were introduced. Each of the isotopologues were distinguished by mass through the incorporation of stable labeled amino acids. For example, peptide VTSGSTSTSR had 5 heavy labelled isotopologues each labeled from heavy 1 to heavy 5 with distinguishable masses (Table 4). The isotopologues were mixed with a 10-fold molar increase enabling an analysis of a wide linear range within a single sample injection.
Method performance for peptide quantification
Significant challenges can arise when quantifying lower abundant peptides in matrix. Improved front-end capabilities of the SCIEX 7500 system present better ion generation and transmission, which facilitates quantification of low-level peptides present in matrix.
Peptide quantification was performed using MRM mode on the SCIEX 7500 system. Each calibration point was measured in 6 replicates. The quantitative criteria for %CV was less than 20% and accuracy was within ±20% of the nominal concentration at the level of the LLOQ. For the remaining concentrations, the %CV was required to be less than 15%, while the accuracy was required to be within ±15% of the nominal concentration.
As shown in Figure 2, the on-column LLOQ for peptides VTSGSTSTSR, LASVSVSR, YVYVADVAAK, VVGGLVALR, LLSLGAGEFK and LGFTDLFSK, were 100 amol, 5 amol, 10 amol, 1 amol, 10 amol and 50 amol, respectively (Table 6). Injection volumes were either 1 µL or 5 µL and are labeled in the LLOQ XICs in Figure 2.
Strong linearity across the peptides was achieved with an overall LDR up to 4.7 orders of magnitude across the linear range tested (Figure 3). Table 6 summarizes the overall quantitative performance on the SCIEX 7500 system.
The hardware improvements on the SCIEX 7500 system provided significant sensitivity gains for peptide quantification facilitating quantification of low-abundant peptides.
Conclusion
- Peptide quantification as low as 1 amol on-column was demonstrated with front-end improvements enabling greater ion generation and transmission on the SCIEX 7500 system
- Low-amol on-column LLOQs for peptide quantification were achieved with GLP accuracy, precision and linearity
- Superior sensitivity was observed for peptide quantification with greater ion generation and transmission provided by the improved front-end technology of the SCIEX 7500 system
References
- Enabling new levels of quantification. SCIEX technical note, RUO-MKT-02-11886-A.
- High sensitivity MRM workflow for signature peptide quantification. SCIEX technical note, RUO-MKT-02-11882-A.