High-performance liquid chromatography

SCIEX high-performance liquid chromatography (HPLC) systems are your go-to solution for physically separating samples before they enter your mass spectrometer. No other company offers the entire range of separation technologies that we do from analytical flow to microflow, to nanoflow LC-MS, or even ultra-low flow CESI-MS. It’s one of the reasons we continue to be a market standout in end-to-end mass spectrometry solutions.

Better data from low to high-flow
Explore the broadest portfolio of mass spectrometer front-ends (LC-MS) and realize the full potential of your lab from sample in to sample out. From low to high-flow, you can be confident that you are not only reducing sample prep time, but cost per sample with a SCIEX HPLC front-end solution that adjusts to the changing demands of your lab.

A history of innovation in mass spectrometry separations
Our history in mass spectrometry separation, also referred to as front-end solutions, began with the development of the first commercially available atmospheric pressure ionization (API) instrument in 1990. Twenty years later, the liquid chromatography capabilities of Eksigent Technologies were acquired, and in 2014, Beckman Coulter’s Capillary electrophoresis systems were also added to the SCIEX portfolio - enabling research labs around the world to evaluate compounds within the pharmaceutical and biopharmaceutical industries, food, environmental and forensics (FEF), and clinical research.

The right data comes from the right front-end

Analytical Flow-LC

For over 30 years scientists have been using analytical flow Liquid Chromatography to develop drugs, monitor food, check water safety, diagnose diseases, and test for drug abuse - making it the Gold Standard Front End to ESI-MS. The ExionLC system offers both HPLC and UHPLC models, and supports a variety of configurations to help you adapt to the changing demands of your laboratory; maximize throughput, and ultimately reducing your cost per sample. Known for its ease of use, speed, and flexibility the ExionLC system appeals to researchers worldwide and can be used in nearly every application area.

Micro LC-MS

Whether you are a bioanalysis researcher looking to increase sensitivity or an applied proteomics researcher in need of improved robustness, M5 MicroLC is your solution. On the one hand, you can achieve your LLOQs while working with smaller animal models. Alternatively, you gain robustness of analytical flow LC-MS for large sample cohorts. In all cases, you achieve quantitation over a larger linear dynamic range, all while decreasing operating costs and increasing your lab’s efficiency.

NanoFlow LC

When you’re looking for the most sensitive form of liquid chromatography, Nanoflow LC enables you to dive deep into proteomics research with mere nanoliters of sample. However, if you’re also looking for a flexible system and range for more robustness and throughput, then the NanoLC 400 series is the right front-end to your ESI-MS. Developed specifically for nanospray mass spectrometry (MS), our NanoLC systems provide speed, sensitivity, resolution, and reliability for your research.


The combination of a proven separation technology, capillary electrophoresis, and the resolving power of mass spectrometry produces a powerful tool to the separation community known as CESI-MS. CESI-MS provides extremely low nanoliter flow rates, minimized ion suppression, increased sensitivity and maximized ion efficiency. This technology gives you a temperature controlled, automated system that requires no stationery phase, has no carryover and no reduction of void volume. The CESI-MS can analyze small molecules to macromolecules while you uncover hidden gems not seen in other technologies.

Analytical flow Microflow Test Nanoflow LC-MS Ultra-low flow CESI-MS
Sample types
A wide range of analytes Small molecules
Intact proteins
Monoclonal antibodies
Proteins Charged & polar analytes
Intact proteins
Isobaric species
Monoclonal antibodies
Main application areas
Food and beverage testing
Forensics testing
Life science research
Pharma and biopharma
Discovery proteomics
Industrialized proteomics
Biopharma characterization

ExionLC™ UHPLC and HPLC Systems

Our ExionLC system provides speed, sensitivity, resolution, and reliability for your routine or complex LC-MS/MS analysis.

M5 Microflow LC System

Innovative system designed for rapid microflow HPLC. Save solvents and reduce your total cost of ownership.

NanoLC™ 400 Series

The NanoLC offers the widest flow rate range of any SCIEX NanoLC system, enabling a diverse collection of workflows on a single HPLC MS instrument.

Ultra-low flow CESI 8000 Plus

With the CESI 8000 Plus you can effectively integrate high-efficiency capillary electrophoresis (CE) with electrospray ionization (ESI) into a single dynamic process within the same device.