iMethod™ Application for Chloramphenicol Version 1.3 for Cliquid® Software

For research use only. Not for use in diagnostic procedures.

Identify and quantify chloramphenicol in seafood and dairy products at trace levels 

This SCIEX iMethod™ Application is a robust method for the quantitation of low levels of chloramphenicol in seafood and dairy products.

Included with this method is a Phenomenex AQUA 5 µm C18 column (125 Å, 50 x 2 mm).

To learn more, please view the flyer: A Rapid iMethod™ Application for Quantitation of Chloramphenicol in Seafood and Dairy Products

iMethod Application for Chloramphenicol Version 1.3 for Cliquid Software


Just download a method, add your sample, and you're ready to go

iMethod™ Applications for food testing provide instant LC-MS/MS methods, enabling you to generate accurate and precise results faster. Simply download a lab-proven method from the web or a CD into Cliquid® Software and run your sample. All the instrument parameters are downloaded upon installation. Everything you need to start running a test immediately is included: sample preparation protocols, optimized MS and LC parameters, integration parameters, reporting templates, and documentation.

Quantitate chloramphenicol in seafood and dairy products 

This iMethod™ Application provides a method for the routine quantitation of chloramphenicol in seafood and dairy products. Chloramphenicol (CAP) an antibiotic, was formerly widely-used in veterinary medicine due to its broad range of activity and low cost. The treatment of animals used for production of food with CAP is prohibited in the European Union and United States because of the side effects in humans; in particular, it can potentially cause fatal aplastic anemia. Thus, it is necessary to control residues of CAP in edible tissues, like milk and milk products.

Examples of sample preparation procedures are provided for milk, milk powder, honey, fish, and seafood. While the actual protocol used is dependent upon the sample matrix, most protocols are based upon homogenization, centrifugation, extraction and dilution. Deuterated chloramphenicol is added as an internal standard during sample preparation to monitor sample recovery. More in-depth sample preparation, instrument configuration, and setting information is included with this iMethod™ Application, upon purchase.

The SCIEX iMethod™ Application for the quantitation of chloramphenicol is configured for use on the SCIEX 3200 and 4000 Series instrumentation.

The list of available iMethod™ Applications located in the iMethod™ Store is continually updated with the newest user-contributed and SCIEX lab-proven methods, so you can take immediate advantage of the latest advances. All iMethod™ Applications for food analysis are available for individual purchase or as part of one of our complete starter packs for food safety testing.

Reduce development time

Activity Using an iMethod Application Traditional Method Development
Complete Test Protocol Documentation Included Weeks–Months
Sample Prep Techniques Included, with examples and references Weeks–Months
Optimized MS/MS Acquisition Parameters Included Weeks–Months
LC Instrument Parameters with Retention Times Included Days–Weeks
Integration (Quantitation) Parameters Included Days
Reporting Templates Included Days
LC/MS/MS Library Spectra May be included. Stand-alone libraries are also available. Weeks–Months
Staff Training – Software Simple Cliquid® Software interface, no additional training Traditional MS Software and review procedures – Days
Method Validation Weeks. Validation templates and support available Weeks–Months
Complete Test Development and Deployment Weeks 6–12 months