January 19, 2023

10 a.m. PT / 1 p.m. ET

Masterclass live: AAV protein profiling and titer determination

Analyzing the viral proteins of recombinant AAV vectors is critical to ensuring the quality and efficacy of gene therapy products. Assessment of the viral protein profile and ratio are critical quality attributes (CQAs) that indicate vector potency and immunogenicity, as well as transduction and packaging efficiency. Rapid and high-resolution analysis is required to test vector quality throughout the development process.

This session will demonstrate the use of the BioPhase 8800 system with high-sensitivity laser-induced fluorescence (LIF) detection as a rapid, method development platform for serotype independent viral protein analysis.  

February 9, 2023

10 a.m. PT / 1 p.m. ET

Masterclass live: Viral protein characterization by peptide mapping

See how use of electron activated dissociation (EAD) combined with the improved sensitivity of the ZenoTOF 7600 system can provide nearly complete sequence coverage of VP1, VP2, and VP3 of rAAV serotype 8. Additionally, learn to confidently identify various modifications like N-terminal acetylation, deamidation and phosphorylation, even in abundance as low as ~0.4%. By implementing a data dependent acquisition (DDA) peptide mapping workflow with automated data acquisition, pinpoint quality attributes that can compromise drug safety and efficacy.

Masterclass live: AAV genome integrity and titer analysis with full and empty capsid ratio

Manufacturing AAV vector-based gene therapies is complex and relies on a robust process to ensure a pure, effective end product. A high percentage of packaged AAV vectors containing the full-length genome is desired, however, capsids containing cellular debris contaminants, host cell DNA and RNA, as well as empty capsids can still be present in the final product. Genome integrity analysis of viral vector-based products enables in-depth quality assessment and sizing of the target encapsidated genome, as well as a fast and efficient way to screen for process and product related AAV impurities. 

This session will demonstrate the use of the BioPhase 8800 system with high-sensitivity laser-induced fluorescence (LIF) detection as an effective tool for genome integrity analysis. Coupling protein titer information generated using the same system, full and empty capsid ratio is also easily calculated.