Five years of SWATH Acquisition yields industry accolades and cutting edge cross-lab research
During the event, SCIEX featured its advanced quadrupole time-of-flight mass spectrometry proteomics platforms, which enable the SWATH acquisition workflow and allows quantification of thousands of proteins across large sample sets with greater accuracy and reproducibility than ever before. The challenge of unlocking the proteome requires innovative tools and technology to detect and quantify more proteins for deeper insights. The SCIEX TripleTOF® 6600 System in combination with SWATH Acquisition delivers the combination of comprehensive coverage with low coefficients of variation previously achievable only by targeted techniques - providing comprehensive high quality quantitative datasets.
SCIEX SWATH Acquisition technology, now celebrating five years since introduced, enables the quantification of thousands of proteins across large sample sets with an unprecedented level of data completeness, quantitative accuracy and reproducibility. SWATH Acquisition allows for comprehensive quantification, which is critical in biomarker or systems biology research. This new technology has transformed proteomics experiments, allowing all data to be captured across very large sample cohorts and now in combination with microflow chromatography, enabling industrialised proteomics. Recently, three primary research articles1 were published, demonstrating the reproducibility of quantitative proteomics data by multiple labs using SWATH Acquisition, discussing how to deal with the false positive inflation issue2, and focusing on post-translational modifications and the inference of peptidoforms algorithm3, respectively.
SCIEX presented work on advancing proteomics research at HUPO2017, including a talk by Stephen Tate and Ron Bonner on the development of the SWATH workflow during the congress award session. SCIEX also hosted a seminar entitled “Biomarkers in Ovarian Cancer from SWATH® maps of clinical samples”. The seminar – which was filled to capacity – gave new insights into use of SCIEX solutions for precision medicine research and quantitative proteomics. The invited speaker was Robert Graham Ph.D., Sr Lecturer in Clinical Proteomics, Deputy Director Stoller Biomarker Discovery Centre, University of Manchester.
Just prior to the conference, SCIEX hosted a VIP round-table discussion focused on the advancement of precision medicine and the major challenges researchers are faced with. Participating in the in-depth discussion are internationally renowned researchers:
- Professor Anthony Whetton, Director, Stoller Biomarker Discovery Centre, University of Manchester, UK
- Professor Jennifer Van Eyk, Director, Advanced Clinical Biosystems Institute, Cedars-Sinai Medical Centre, Los Angeles, USA
- Professor Phil Robinson, Co-leader, PROCAN, Children’s Medical Research Institute, Sydney, Australia
- Professor Thomas Conrads, Chief Scientific Officer, Women’s Health Integrated Research Center, Inova Health System, Falls Church, USA
- Associate Professor Mark Malloy, Department of Chemistry & Biomeolecular Sciences, Macquarie University, Sydney, Australia
“We are pleased to have organised and facilitated so many important discussions with researchers, some from the leading edge of precision medicine to those who are interested in high-performance solutions for quantitative proteome analysis at HUPO2017” said Mark Cafazzo, Director of Academic and Applied Markets at SCIEX. “We are also happy to celebrate the fifth anniversary of our unique SWATH Acquisition technology and we believe that our cutting-edge technologies and applications will continue to help customers examine the proteome more completely, addressing diverse needs across the research pipeline. We are committed to advancing the leading edge of biomarker research in order to unlock knowledge that will positively impact more people’s lives.”
- Collins, BC et al., Multi-laboratory assessment of reproducibility, qualitative and quantitative performance of SWATH-mass spectrometry (2017) Nature Communications 8, doi:10.1038/s41467-017-00249-5
- Rosenberger, G. et al. Statistical control of peptide and protein error rates in large-scale targeted data-independent acquisition analyses. (2017) Nature Methods, doi:10.1038/nmeth.4398.
- Rosenberger, G. et al. Inference and quantification of peptidoforms in large sample cohorts by SWATH-MS (2017) Nature Biotechnology 35, 781–788.