Targeted proteomics: detect proteins of interest with high sensitivity, accuracy and reproducibility

Reliable validation of biological relevance
 

SCIEX solutions for targeted proteomics are driven by the use of our renowned MS/MS sensitivity and precision for MRM and MRMHR approaches for targeted protein quantification and biomarker verification/validation.

The goal of targeted proteomics is to analyze a key set of proteins across large numbers of samples to verify and validate specific biological hypotheses. The ability to analyze larger sample sets enables longitudinal studies, validates biomarkers of disease and drives translational research. Asking specific questions about a subset of proteins across many samples requires high sensitivity, quantitative accuracy and reproducibility to establish biological significance.

MRMHR and MRM approaches are the most sensitive and accurate approaches to relative and absolute protein quantification. Combined with quantitative robustness and high-throughput analysis, both large and small biological changes can be accurately measured for extensive protein panels across large sample cohorts.

Large-scale targeted proteomics without compromise

Large-scale targeted proteomics without compromise
To gain meaningful information that confirms or disproves the utility of differentially expressed proteins/peptides in specific cell and disease conditions, researchers need the capability to extract even the low-abundance proteins in every sample, at high throughput and with continued reproducibility and precision.

Measure precise differences characteristic of diseases
High-resolution, high mass accuracy, full-scan MS and very high sensitivity MS/MS data significantly increase the depth of information visible in every sample, even for low-abundance proteins, which enables precise characterization.

Harness the throughput needed for extensive protein panels
As more extensive protein panels need to be monitored in a targeted way across multiple samples, higher multiplexing of MRM transitions is essential for throughput enabling large-scale peptide quantification for targeted biomarker research.

Accurately measure biological change in large sample sets
Quantitative robustness and the ability to detect low limits over a wide range of protein abundance remain key to ensuring that both large and small biological changes are accurately measured across large sample cohorts.

Large-scale, targeted, peptide quantification of 804 peptides with high reproducibility

Using a 20-minute microflow gradient with a Scheduled MRMHR workflow on the ZenoTOF 7600 system

Reproducible targeted peptide profiling using highly multiplexed MRM assays

Using SCIEX Triple Quad 7500 LCMS/MS system − QTRAP Ready

Highly sensitive quantification of proteins from the SARS-CoV-2 antigen in nasopharyngeal swab samples

Using the SCIEX Triple Quad 7500 LC-MS/MS system – QTRAP Ready

High selectivity quantification of peptides

Using SelexION Differential Mobility Separation technology on QTRAP systems

Precise, sensitive solutions for high-throughput protein quantification

Highly multiplexed and sensitive targeted protein assays require solutions that can deliver reproducible, robust quantification, and methods that meet the throughput demands of large sample sets. Whether derived from biological hypotheses or from discovery data (for example, from SWATH acquisition), the ZenoTOF 7600 and SCIEX 7500 systems deliver precise targeted quantification via MRMHR and MRM approaches, respectively. Method development timelines are shortened by using SWATH acquisition on the ZenoTOF 7600 system to generate target methods for proteins of interest, while QTRAP mass spectrometer technology on the SCIEX 7500 system can be used to verify peptide identities during the development of MRM methods. OptiFlow source technology gives access to the low flow regimes on each system to help ensure that required throughput and sensitivity can be balanced according to the needs of the assay.

Qualitative flexibility combined with quantitative power

A crucial step change in MS/MS technology, the ZenoTOF 7600 system unlocks new, precise perspectives on the proteome.

GO BEYOND

The SCIEX 7500 system offers the high sensitivity and wide linear dynamic range you need for largescale targeted peptide quantification.

A revolution in low-flow separations to maintain the perfect balance of robustness and sensitivity

Discover the OptiFlow Turbo V ion source: an easy-touse, low-flow source technology with cutting-edge features.

Switch to SelexION Differential Mobility Separation technology

Experience added selectivity and specificity using SelexION DMS technology

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