Advanced quantitative approaches made easy with the TripleTOF® 6600+ System

Achieve the optimum of quantitative performance

Built and optimized for large-scale quantitative mass spectrometry, the TripleTOF 6600+ System offers sensitive and robust quantitation using dedicated low flow source technology. Redesigned TOF entrance optics, along with software improvements within the forthcoming Analyst® TF Software 1.8, deliver optimized ion beam control, detector tuning, and scheduled ionization to decrease the risk of downtime over long term usage. Combined with the characteristically fast scanning capability of previous TripleTOF platforms, SWATH® Acquisition, and the ultimate approach for data-independent digital archiving of samples, the instrument you see before you could unlock a world of quantitative data you have never experienced before.

TripleTOF 6600+ System

Built for large-scale quantification and flexible use


New source hardware designed to cater to the varied requirements of core and research labs, the TripleTOF 6600+ delivers both flexible and easy to use low flow ionization for optimal sensitivity and throughput for a given sample set. Combined with the quantitative power of current and future SWATH Acquisition methods, the TripleTOF 6600+ System has the performance to deliver sensitive, large scale quantitative data for any application.

Key features

OptiFlow Turbo V SourceA single source for all low flow applications. SteadySpray™ analytical, micro and nano probes allow the source to deliver flow rates from 200 µL/min down to 100 nL/min, while optimized heater geometry eliminates the need for optimizing spray position.
OptiFlow InterfaceTool-free switching between flow regimes. OptiFlow Interface allows you to easily select the most appropriate flow rate for your experiment without the hassle of breaking vacuum.
TripleTOF System scan speedUp to 100Hz MS/MS acquisition gives the system unique qualitative and quantitative capabilities, from fast targeted quantitation to highly multiplexed methods. Fast DDA approaches allow more information to be captured from shorter run times, while SWATH Acquisition, with up to 200 windows per cycle, is capable of producing complete digital records of samples, covering all observable species in a dataset that can be retrospectively mined over and over.
Analyst TF Software 1.8New functionality for scheduling ion spray voltages allows the user to control when LC flow is sprayed into the instrument reducing the introduction of potentially contaminating species from your LC. New optimized ion transmission control and detector optimization algorithm give added control over the flux of ions through the instrument to reduce the risk of downtime.
Primed for the evolution of SWATH AcquisitionVariable-window SWATH Acquisition offers the latest in data independent acquisition, delivering sensitive quantitative data on a large scale across all observable species, allowing the user to create a permanent digital record of their sample that can be interrogated over and over. The TripleTOF 6600+ System is the platform that will host the next generation of SWATH Acquisition.

Choose the right flow rate for the right job with Optiflow

Optimum performance for microflow applications

OptiFlow source and OptiFlow interface are built for simple, accessible low flow applications that deliver high sensitivity quantitative and qualitative data. The OptiFlow Turbo V ion source allows the robust and routine implementation of microflow LC-MS methods for high sensitivity quantitation, large-scale, sensitive metabolite profiling and high throughput proteomics methods using intelligent probe technology and optimized SteadySpray™ micro and analytical flow probes to ensure no optimization of the probe is needed and that a stable spray can be reproducibly achieved time after time.

Easy to use nanoflow for challenging sample types

The OptiFlow Turbo V Source will also uniquely allow the user to switch to nanoflow regimes for the highest sensitivity of proteomics samples where sample amount or concentration makes their analysis challenging such as low volume biopsy samples, IP pulldowns or detection of low abundance proteins in a targeted analysis. Using the forthcoming SteadySpray nano probes, the OptiFlow Turbo V Source delivers the high performance nanospray that these sample types demand, with minimal downtime and setup time.

The right flow rate for the right job

Choosing the right flow rate to tackle a specific analytical challenge is simple with OptiFlow. Switching between low flow regimes no longer requires breaking vacuum, changing source or re-optimisation of source parameters, allowing you to change between the flow rates appropriate for the job at hand without compromise.

Optiflow Turbo V Source for microflow

A revolution in microflow separations, the Optiflow Turbo V Ion Source supports flow ranges from 1 to 200 µL/min, with a simple tool-free set-up and no manual adjustments needed to achieve optimal spray conditions.

Optiflow Interface

Switch between flow rate regimes in minutes with Optiflow Interface. Tool-free fitting of the appropriate components allows simple switching between microflow and nanoflow regimes without the need to break vacuum.

Optiflow Turbo V Source for nanoflow

Simple, plug-and-play nanoflow capability takes the hassle out of nanospray applications. Integrated column oven and SteadySpray nanoprobes allow easy setup of nanoflow applications with no need for optimization.

Precision medicine and industrialized OMICS

Precision medicine and industrialized OMICS

The ability to acquire and process comprehensive multi-OMICS data in a high-throughput manner opens the door to large clinical studies in precision and stratified medicine via large-scale biomarker discovery in a manner that can be verified across thousands of samples. Microflow approaches give the ideal balance of sensitivity and throughput for protein and metabolite analysis, while SWATH Acquisition allows large-scale, precise quantitative analysis.

Large-scale quantitative proteomics with the TripleTOF 6600+

Optimized high-throughput microflow, SWATH Acquisition and fast data processing with OneOMICS™ in SCIEX Cloud. The ultimate end to end solution for biomarker researchers.

Accelerated proteomics

Rapid quantitative profiling of up to 100 samples per day using microflow chromatography with variable windowed SWATH Acquisition.

Digitizing biobanks

SWATH Acquisition for large-scale digitization of big tissue biobanks.

Core OMICS and life science research

Core OMICS and life science research

The versatility of the TripleTOF 6600+ System to deliver low-flow applications with minimal setup and optimization allows core facilities with diverse OMICS workloads to select the optimal approach for each experiment and subsequently deliver superior results to collaborators in a timely fashion. In combination with high-performance analytical tools such as SWATH Acquisition and collaborative, high-performance data processing platforms on SCIEX Cloud, core facilities and life science researchers can read.

A single source solution for low flow chromatography

Easily switch between flow regimes, while maintaining high robustness and sensitivity with the OptiFlow Turbo V Source.

Fast whole proteome profiling with microflow

Deep mining of proteomes that can be achieved overnight. Microflow enables protein identification using a fractionation approach in just 15 hours of acquisition time.

Quantitative proteomics in CRISPR gene editing

SWATH Acquisition for interrogating protein changes induced by CRISPR-Cas9 gene editing.

Drug discovery and development

Drug discovery and development

Targeted high-resolution approaches offer distinct advantages for quantification of drugs and drug metabolites in enhanced selectivity for complex matrices and the simplicity of method development. Low flow quantitative approaches using the OptiFlow Turbo V Ion Source deliver these approaches with high sensitivity for bioanalytical and compound screening workflows, and offer distinct advantages in the detection and quantitation of low level drug metabolites in ADME studies.

Enhanced quantitation of challenging analytes

Enhanced quantitation of challenging analytes

Benefits of high-resolution quantitation

Selectivity with no method development: discover why high-resolution MRM approaches are the most flexible for targeted quantitation.

The key to more sensitive met ID

Improve, identify, and characterize more small molecule metabolites with the use of OptiFlow Source and a TripleTOF 6600+ System.

Related products

OptiFlow Turbo V Ion Source

A revolution in microflow separations, the OptiFlow Turbo V Source supports flow ranges from 1 to 200 uL/min, with a simple tool-free set-up and no manual adjustments needed to achieve optimal spray conditions.

OptiFlow Interface

A universal interface that allows fast switching between nanoflow, microflow and analytical flow regimes without the need to break vacuum on the mass spectrometer system.

SWATH Acquisition

The only data independent acquisition (DIA) technique that allows comprehensive detection and quantitation of virtually every detectable compound in a sample (MS/MSALL). SWATH Acquisition gives you confidence in your quantitative results and virtually eliminates the risk of missing a critical component.

M5 MicroLC

Increase your productivity to routinely analyze hundreds of compounds in hundreds of samples – day after day, year after year with the M5 MicroLC System.

NanoLC 400 Series

No longer choose between the high sensitivity of nanoflow and the high-throughput of microflow. Use one flexible system to move from discovery to high-throughput targeted assays.

Differential ion mobility technology

The SelexION® Technology is an effective differential mobility separation tool for improving data quality for qualitative and quantitation analysis of challenging samples requiring advanced analytical selectivity.