AB Sciex is doing business as SCIEX. © 2010-2018 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.
For research use only. Not for use in diagnostic procedures.
SCIEX nano cHiPLC columns are made with robustness and ease-of-use in mind. Our manufacturing process uses a unique weir structure to retain the stationary phase particles in the column. These weirs are more reproducible to fabricate, while their dead-volume is virtually zero (~13 pL). Compared with nanoLC columns constructed from fused silica tubing with end-frits made by sintering stationary phase particles, the cHiPLC columns are much more robust and far easier to handle and connect without introducing dead volumes.
Special care has been given to the design of both the trap-chips and analytical column-chips in order to achieve separations that are equal or better than separations obtained using packed capillaries. The use of fused silica allows for cylindrical channels for packing nanoLC columns and traps. Instead of conventional frits made out of fused stationary phase particles, our cHiPLC columns use a unique weir structure to retain the stationary phase particles in the column. These weirs are more reproducible to fabricate, while their dead-volume is virtually zero (~13 pL). In addition, adsorption of sample components that can occur with frit material is not an issue with these types of structures.
Patented connection system
Connections to and from each chip are made using a patented connection system that can connect up to seven channels to the outside world with a dead volume of less than 1 nl. The force used to connect the chip is pre-set, so that every time the user exchanges a chip, a specifically leak-free connection is obtained without any required user adjustments.
Increased column-to-column reproducibility
Besides the ease of replacing a nanoLC column or trap in seconds, the use of our cHiPLC columns also increases column-to-column reproducibility. All chips are exactly identical, and our packing procedure guarantees the best possible column-to-column reproducibility in nanoLC. This is of importance for applications where retention time stability over longer periods of time and over multiple columns is important. Examples are the use of retention time in combination with accurate mass in peptide/protein identification and scheduling MRM's for peptide quantitation in biomarker verification.
|cHiPLC Columns||Part Number|
|Nano cHiPLC column 75µm x 15cm ChromXP C18-CL 3µm 120Å||804-00001|
|Nano cHiPLC column 75µm x 15cm ChromXP C18-CL 3µm 300Å||804-00003|
|Nano cHiPLC column 75µm x 15cm ChromXP C18-CL 5µm 120Å||804-00002|
|Nano cHiPLC column 75µm x 15cm ChromXP C18-CL 5µm 300Å||804-00004|
|Nano cHiPLC column 75µm x 15cm ReproSil-Pur C18-AQ 3µm 120Å||804-00011|
|Nano cHiPLC column 75µm x 15cm ChromXP C8-CL 3µm 120Å||804-00005|
|Nano cHiPLC column 75µm x 15cm ChromXP C4-CL 5µm 300Å||804-00018|
|Nano cHiPLC column 75µm x 15cm Graphitized Carbon 3µm 250Å||804-00020|
|Nano cHiPLC column 75µm x 15cm HALO HILIC||804-00022|
|Trap Columns||Part Number|
|Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C18-CL 3µm 120Å||804-00006|
|Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C18-CL 3µm 300Å||804-00008|
|Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C18-CL 5µm 120Å||804-00007|
|Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C18-CL 5µm 300Å||804-00009|
|Nano cHiPLC Trap column 200µm x 0.5mm ReproSil-Pur C18-AQ 3µm 120Å||804-00016|
|Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C8-CL 3µm 120Å||804-00010|
|Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C4-CL 5µm 300Å||804-00019|
|Nano cHiPLC Trap column 200µm x 0.5mm Graphitized Carbon 3µm 250Å||804-00021|
|Nano cHiPLC Trap column 200µm x 0.5mm HALO HILIC||804-00023|
|Chip based particle trap (weir) (between autosampler and cHiPLC nanoflex)||800-00354|
|Jumper Chips||Part Number|
|Direct injection jumper chip||800-00408|
|Trap-elute jumper chip||800-00389|
|Dual column jumper chip||800-00421|
|Micro/Nano Columns Part List||08/21/2018|
|SCIEX Defined Major Repairs||01/10/2016|
|Extending Depth of Coverage with SWATH Acquisition Using Deeper ION Libraries||12/10/2015|
|Improving Proteomics Reproducibility using Chip-based Nanoflow Chromatography||11/01/2015|
|Increasing Dept of Coverage Using Serial Two-Column Workflows||11/01/2015|
|Brochure: Micro LC and cHiPLC Columns||01/01/2013|
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