Nano cHiPLC® Columns

For research use only. Not for use in diagnostic procedures.

SCIEX nano cHiPLC columns are made with robustness and ease-of-use in mind.  Our manufacturing process uses a unique weir structure to retain the stationary phase particles in the column. These weirs are more reproducible to fabricate, while their dead-volume is virtually zero (~13 pL). Compared with nanoLC columns constructed from fused silica tubing with end-frits made by sintering stationary phase particles, the cHiPLC columns are much more robust and far easier to handle and connect without introducing dead volumes.

  • Easy: Plug & play chip simplicity with the performance of a nano column
  • Extendable: Flexibility to switch between workflows and projects rapidly in multi-user labs
  • Every time: Reproducible results from day-to-day, column-to-column and lab-to-lab

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Nano cHiPLC Columns

Overview

Column design

Special care has been given to the design of both the trap-chips and analytical column-chips in order to achieve separations that are equal or better than separations obtained using packed capillaries. The use of fused silica allows for cylindrical channels for packing nanoLC columns and traps. Instead of conventional frits made out of fused stationary phase particles, our cHiPLC columns use a unique weir structure to retain the stationary phase particles in the column. These weirs are more reproducible to fabricate, while their dead-volume is virtually zero (~13 pL). In addition, adsorption of sample components that can occur with frit material is not an issue with these types of structures.

Innovative connection system

Connections to and from each chip are made using a innovative connection system that can connect up to seven channels to the outside world with a dead volume of less than 1 nl. The force used to connect the chip is pre-set, so that every time the user exchanges a chip, a specifically leak-free connection is obtained without any required user adjustments.

Increased column-to-column reproducibility

Besides the ease of replacing a nanoLC column or trap in seconds, the use of our cHiPLC columns also increases column-to-column reproducibility. All chips are exactly identical, and our packing procedure guarantees the best possible column-to-column reproducibility in nanoLC. This is of importance for applications where retention time stability over longer periods of time and over multiple columns is important. Examples are the use of retention time in combination with accurate mass in peptide/protein identification and scheduling MRM's for peptide quantitation in biomarker verification.

Parts

Nano cHiPLC columns and trap columns are available in a range of stationary phases and column lengths.

cHiPLC Columns Part Number
Nano cHiPLC column 75µm x 15cm ChromXP C18-CL 3µm 120Å 804-00001
Nano cHiPLC column 75µm x 15cm ChromXP C18-CL 3µm 300Å 804-00003
Nano cHiPLC column 75µm x 15cm ChromXP C18-CL 5µm 120Å 804-00002
Nano cHiPLC column 75µm x 15cm ChromXP C18-CL 5µm 300Å 804-00004
Nano cHiPLC column 75µm x 15cm ReproSil-Pur C18-AQ 3µm 120Å 804-00011
Nano cHiPLC column 75µm x 15cm ChromXP C8-CL 3µm 120Å 804-00005
Nano cHiPLC column 75µm x 15cm ChromXP C4-CL 5µm 300Å 804-00018
Nano cHiPLC column 75µm x 15cm Graphitized Carbon 3µm 250Å 804-00020
Nano cHiPLC column 75µm x 15cm HALO HILIC 804-00022
Trap Columns Part Number
Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C18-CL 3µm 120Å 804-00006
Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C18-CL 3µm 300Å 804-00008
Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C18-CL 5µm 120Å 804-00007
Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C18-CL 5µm 300Å 804-00009
Nano cHiPLC Trap column 200µm x 0.5mm ReproSil-Pur C18-AQ 3µm 120Å 804-00016
Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C8-CL 3µm 120Å 804-00010
Nano cHiPLC Trap column 200µm x 0.5mm ChromXP C4-CL 5µm 300Å 804-00019
Nano cHiPLC Trap column 200µm x 0.5mm Graphitized Carbon 3µm 250Å 804-00021
Nano cHiPLC Trap column 200µm x 0.5mm HALO HILIC 804-00023
Chip based particle trap (weir) (between autosampler and cHiPLC nanoflex) 800-00354
Jumper Chips Part Number
Direct injection jumper chip 800-00408
Trap-elute jumper chip 800-00389
Dual column jumper chip 800-00421