Primer Purity Requirements for Sequencing and Fragment Analysis Using the GenomeLab™ GeXP Genetic Analysis System

For research use only. Not for use in diagnostic procedures.

Answer

When ordering nucleic acid primers for use in either DNA sequencing or fragment analysis using the GenomeLab™ GeXP Genetic Analysis System or older CEQ genetic analyzers, be sure to select primers that—at the minimum —have been purified via solid phase extraction. De-salted primers will not work well for sequencing, but they can be used for fragment analysis. Further purification techniques, such as reversed-phase or anion-exchange HPLC or even higher PAGE gel purification, can also be used but are only necessary if the primer is highly specific to its binding site on the template.