Converting WIFF2 Files from SCIEX OS Software to the mzML and mzXML Format


Date: 05/17/2022
Categories: Analyst software

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For research use only. Not for use in diagnostic procedures.


Answer

To convert WIFF2 files created by SCIEX OS software to the mzML and mzXML format, a conversion tool must be used. The conversion tool found on the ProteoWizard webpage (http://proteowizard.sourceforge.net/downloads.shtml) has recently been updated to work with WIFF2 files from SCIEX OS software and can convert them to the mzML and mzXML format.

Posted: Mar 20, 2025

Hello Sciex team, as the user below, I also have issues in converting WiFF files from Sciex OS 3.4 using ProteoWizard/MSConvert. Could you please help with this issue?


Posted: Oct 29, 2024

Hello SCIEX team, could you verify if this is still true? I'm using SCIEX OS 3.4 for acquisition, but I can't get ProteoWizard/MSConvert to read my WIFF2 file. I can write mzML from the .wiff file, either using MSConvert or the MS Converter v.1.3.1; however, the charge state information doesn't seem to be captured in the mzML file. Thanks for your advice!