How do I search an EST or other nucleotide database


Date: 12/07/2016
Categories: Academia Omics , ProteinPilot Software

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For research use only. Not for use in diagnostic procedures.
Answer 

You have to translate the database first. ProteinPilot software cannot directly search nucleotide databases. There are several publicly available tools to do six-frame translation. For example, you can go to: http://www.ebi.ac.uk/Tools/emboss/transeq/index.html and use the 'Upload a file' option below the sequence entry field.