How to calibrate MS3 in negative mode in Analyst® software
| Date: | 04/18/2024 |
| Categories: | QTRAP systems , Analyst software |
For research use only. Not for use in diagnostic procedures.
Answer
Requirements:
- Calibrate Q1 and Q3 in positive and negative modes
- Calibrate LIT in positive and negative modes (select the appropriate scan rate)
- Verify MS3 positive mode with Reserpine according to service guide. Calibrate Isolation and Excitation coefficients for MS3 positive mode if necessary (Use Instrument Optimization with Agilent Tuning Mix solution)
Procedures:
- Click on Manual Tuning
- Navigate to Tools/Settings/Instrument Options
- Select LIT mode and positive polarity and Scan Speed (select the appropriate scan rate)
- Click on Advanced Settings
- Copy MS3 Isolation Coefficient Calibration Table and MS3 Excitation Coefficient Calibration Table and Resolution Table in Excel file
- Close Advanced Calibration Tables window
- Select negative polarity
- Click on Advanced Settings
- Paste MS3 Isolation Coefficient Calibration Table and MS3 Excitation Coefficient Calibration Table and Resolution Table from positive mode
There isn’t a solution in PPG Kit to verify MS3 negative performance.
But customer can provide a standard which give good result in MS2 in negative mode, example:
Use this standard to test MS3 negative mode:
- Use the precursor ion (from MS2) for 1st precursor and 2nd precursor, example:
- Run MS3 (Ramp AF2 if necessary). Fragments found in MS2, should be detected in MS3, example:
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