How to create a Shimadzu LC method using the internal rinse feature ?

For research use only. Not for use in diagnostic procedures.

Answer

There are two rinse ports (immersion and flow). In simplest terms, the immersion rinse port allows you to dip the needle into a rinse solvent (R0). Commonly, R0 is mobile phase at the beginning of the gradient. A multi-rinse option (equipped as standard on the SIL-40C X3) allows you to use an active flow rinse port to clean the outer surface of the needle with a different combination of rinse solvents (R1 and R2). The R1 and R2 could set up a rinsing sequence of strong solvent (R1) followed by a weaker solvent (R2). The multi-rinse option may also use an active rinse solvent (R3), which controls cleaning with an active diaphragm pump. When R3 is used the volume of solvent used is important because the pump uses a large flow of this washing solution. It is therefore recommended to use bottles with a large volume to contain R3 (one liter bottle).

*1 : Only the rinse solution R0 is available without a multi-rinse option. With a multi-rinse option, selection one solution from R0, R1, or R2.
*2 : Only one rinse solution (R0) is available without a multi-rinse option. With a multi-rinse option, three rinse solutions (R0, R1, and R2) are available. It is recommended to use the mobile phase for the rinse solution R0.

The settings described below are related to the "external and internal rinse" feature for a Shimdazu LC used by the SCIEX software (SCIEX OS, Analyst®) and the Shimdazu driver :

Example for the “Sample Loop Equilibration” feature :

Caution for internal rinse :