Applying an incorrect dilution factor in a results table in Analyst software gives an incorrect calculated concentration

For research use only. Not for use in diagnostic procedures.

Issue Description

In this example, two sets of samples of known concentration were acquired and the calculated concentration values were shown incorrectly in the results table. In the first set of quality control samples (rows 1-3), the actual and calculated concentration values were very similar. However, the second set of quality control samples (rows 4-6) shows a much higher calculated concentration compared to the actual concentration.

Resolution

If the calculated concentration is higher than expected, check the dilution factor and ensure that it is entered correctly in the results table. In the example shown, the dilution factor was set to 50 for the second set of quality control samples instead of the expected value of 1, which was used for the first set of quality controls. When the dilution factor was updated to 1, the expected values for calculated concentration were similar for both sets of quality control samples.